1887

Abstract

A tangerine-coloured, Gram-positive actinobacterial strain, designated F10, was isolated from the abdominal epidermis of a sea cucumber, , collected in seawater off the coast of Japan. A 16S rRNA gene sequence analysis indicated that strain F10 was a member of the class and was most closely related to ANL-iso2 (87.4 % sequence similarity). Phylogenetic analyses showed that strain F10 represented a novel, deep-rooted, and distinct phylogenetic lineage within the class and clustered with and uncultured bacteria. The organism had -diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan, and rhamnose and galactose as the diagnostic cell-wall sugars. Strain F10 contained C 7, C and C 8 as the major cellular fatty acids. The predominant isoprenoid quinone was MK-9 (H). The G+C content of the DNA was 68.3 mol%. Based on data from the current polyphasic study, it is proposed that the new marine isolate be placed in a novel genus and be considered a novel species designated gen. nov., sp. nov. within the new family, order and subclass fam. nov., ord. nov. and subclassis nov. in the class . The type strain of is F10 (=NBRC 105439 =KCTC 19736).

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2010-10-01
2019-10-21
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vol. , part 10, pp. 2314 - 2319

Transmission electron micrograph showing the general morphology of cells of strain F10 grown on MA for 4 weeks at 25 °C, negatively stained with uranyl acetate. Bar 500 nm.



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