1887

Abstract

A novel actinomycete, designated strain 5414T-18, was isolated from an air sample collected from the Taean region, Korea. The strain contained oxidase and grew in the presence of 7 % NaCl. A neighbour-joining tree constructed on the basis of the 16S rRNA gene sequence showed that strain 5414T-18 is a member of the genus , sharing 97.8–98.3 % 16S rRNA gene sequence similarities to type strains of species of the genus (98.3 % sequence similarity with LR-26). It contained peptidoglycan containing -diaminopimelic acid of A3 type, with three glycine residues as the interpeptide bridge. Whole-cell sugars were glucose, mannose and ribose. Mycolic acids were absent. The predominant menaquinone was MK-8(H). The major fatty acids (>7 % of total fatty acids) were iso-C, iso-C, C 8 and iso-C. The major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol and an unidentified phosphoglycolipid. The DNA G+C content of the type strain was 73 mol%. Strain 5414T-18 exhibited DNA–DNA relatedness levels of 44, 43, 39, 34 and 34 % to the type strains of , , and , respectively. These findings suggest that strain 5414T-18 represents a novel species within the genus . The name sp. nov. is proposed for this novel species, with the type strain 5414T-18 (=KACC 20693=DSM 18563).

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2010-05-01
2019-10-14
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