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Abstract

A Gram-stain-positive, neutrophilic, rod-shaped bacterium, strain A1g, was isolated from activated sludge of a bioreactor and was subjected to a polyphasic taxonomic characterization. The isolate grew in the presence of 0–17.0 % (w/v) NaCl and at pH 6.0–9.0; optimum growth was observed in the presence of 3.0–5.0 % (w/v) NaCl and at pH 7.0. Strain A1g was motile, formed cream-coloured colonies, was catalase- and oxidase-positive and was able to hydrolyse aesculin, Tween 40 and Tween 60. Chemotaxonomic analysis revealed menaquinone-7 as the predominant respiratory quinone and anteiso-C, anteiso-C, iso-C and iso-C as major fatty acids. The genomic DNA G+C content of strain A1g was 36.3 mol%. Comparative 16S rRNA gene sequence analysis revealed that the new isolate belonged to the genus and exhibited closest phylogenetic affinity to the type strains of subsp. (97.9 % similarity) and subsp. (97.5 %), but less than 97 % sequence similarity with respect to the type strains of other recognized species. Levels of DNA–DNA relatedness between strain A1g and reference strains subsp. DSM 16557, subsp. JCM 12661 and DSM 14371 were 29, 45 and 38 %, respectively. On the basis of phenotypic and genotypic data, strain A1g is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is A1g (=CGMCC 1.7693 =JCM 15776).

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2010-10-01
2019-10-20
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vol. , part 10, pp. 2409 - 2414

Phylogenetic trees based on 16S rRNA gene sequences reconstructed using the maximum-parsimony (a) and maximum-likelihood (b) methods. [PDF](38 KB)

Fatty acid compositions (%) of strain A1g and the type strains of related members of

Strains: 1, A1g ; 2, subsp. DSM 16557 ; 3, DSM 14371 . Data were obtained in the present study; the three strains were grown under identical conditions on MA for 48 h at 35 °C. Fatty acids representing <1 % for all strains are not shown. –, Not detected.



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