1887

Abstract

Ten non-motile, Gram-stain-positive, coagulase-negative staphylococci were isolated from bovine milk and teat apices. All isolates were catalase-positive, with anteiso-C, iso-C, anteiso-C, iso-C and C as predominant fatty acids and diphosphatidylglycerol and phosphatidylglycerol as major polar lipids. The results of sequence analysis of the 16S rRNA gene and four housekeeping genes (, , and ) in combination with tRNA-intergenic spacer length analysis showed that the isolates form a separate branch within the genus . Based on 16S rRNA gene sequencing, the phylogenetically most closely related species are , and , with >98.7 % sequence similarity. The DNA G+C content varies from 33.3 to 33.7 mol%, and DNA–DNA hybridization with the nearest neighbours, based on 16S rRNA gene sequences, confirmed that the isolates represent a novel species. All isolates induced a small zone of complete haemolysis on Columbia agar with 5 % sheep blood and exhibited a homogeneous biochemical fingerprint that is discriminative from the phylogenetically most closely related species. Based on these results, it is proposed to classify the ten isolates as sp. nov., with strain KS-SP 60 (=LMG 25332 =CCUG 58238) as the type strain.

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2010-12-01
2020-01-26
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vol. , part 12, pp. 2739 - 2744

Dendrogram constructed on the basis of tRNA-intergenic spacer length patterning (UPGMA algorithm), showing the position of strains of sp. nov. amongst representatives of the genus .

Phylogenetic tree, constructed using the neighbour-joining method, based on gene sequences of strains of sp. nov. and reference sequences of strains available online.

Phylogenetic trees, constructed using the neighbour-joining method, based on (a), (b) and (c) gene sequences of strains of sp. nov. and reference sequences available online from strains of the phylogenetically most closely related species based on the comparison of sequences of 16S rRNA and four housekeeping genes ( , , and ).

Geographical origin and source of isolation of isolates of sp. nov.

Primers used in this study for amplification and sequencing.

Phenotypic characteristics of the ten individual strains of sp. nov.

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