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Volume 60, Issue 5

sp. nov., a nitrogen-fixing bacterium isolated from an aquatic plant, , that grows in highly acidic swamps in actual acid sulfate soil areas of Vietnam Free

Abstract

Nitrogen-fixing bacteria, strains SA41, SA42 and SA53, were isolated from an aquatic plant, , that grows in highly acidic swamps (pH 2–4) in actual acid sulfate soil areas of Vietnam. The isolates were Gram-negative, aerobic, non-spore-forming, rod-shaped bacteria, having a cell width of 0.6–0.7 μm and a length of 1.5–1.7 μm. They showed good growth between pH 3.0 and 7.0, and between 17 and 37 °C. The organisms contained ubiquinone Q-8 as the predominant isoprenoid quinone, and C, C cyclo, C 7 and summed feature 3 (C 7 and/or iso-C 2-OH) as major fatty acids. Their fatty acid profiles are similar to those reported for other species. The DNA G+C content of these strains was 64 mol%. On the basis of 16S rRNA gene sequence similarity, these strains were shown to belong to the genus Although their calculated 16S rRNA gene sequence similarity values to and were 98.5, 98.2, 98.0 and 97.0 %, respectively, the isolates formed a distinct group in phylogenetic trees, and the DNA–DNA relatedness values of strain SA41 to these species were 39, 41, 39 and 33 %, respectively. The results of physiological and biochemical tests, including whole-cell protein pattern analysis, allowed phenotypic differentiation of these strains from the published species. Therefore, strains SA41, SA42 and SA53 represent a novel species for which the name sp. nov. is proposed. The type strain is SA41 (=NBRC 101817=VTCC-D6-7).

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Keyword(s): AASS, actual acid sulfate soil(s)
SGM
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2010-05-01
2024-05-10
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Burkholderia heleia sp. nov., a nitrogen-fixing bacterium isolated from an aquatic plant, Eleocharis dulcis, that grows in highly acidic swamps in actual acid sulfate soil areas of Vietnam
Int J Syst Evol Microbiol 60, 1152 (2010); https://doi.org/10.1099/ijs.0.015198-0
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