1887

Abstract

Two clinical strains (A2-50 and A2-67) recovered from the wounds of two patients in Taiwan could not be assigned to any known species of this genus based on their 16S rRNA gene sequences, which showed similarities of 99.6–99.8 % to those of the type strains of , and . The phylogenetic tree allocated these strains to two novel and independent phylogenetic lines, the neighbouring species being , the type strain of which showed 93.2 % similarity (56 bp differences) to strain A2-50 and 92.2 % (63 bp differences) to strain A2-67. A multilocus phylogenetic analysis of five housekeeping genes (, , , and ; 3684 bp) confirmed that the two strains formed independent phylogenetic lineages within the genus. These data, together with phenotypic characterization and DNA–DNA reassociation results, revealed that these strains represent novel species, for which the names sp. nov. (type strain A2-50 =CECT 7403 =LMG 24683) and sp. nov. (type strain A2-67 =CECT 7402 =LMG 24682) are proposed.

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2010-09-01
2019-12-08
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vol. , part 9, pp. 2048 - 2055

Unrooted maximum-parsimony phylogenetic tree derived from 16S rRNA gene sequences (1503 nt) showing the relationships of strains A2-50 ( sp. nov.) and A2-67 ( sp. nov.) to currently known species of .

Unrooted minimum-evolution phylogenetic tree derived from MLPA ( , , , and ) showing the relationships of strains A2-50 ( sp. nov.) and A2-67 ( sp. nov.) to currently known species of .

Base differences in the 16S rRNA gene sequences of strains A2-50 ( sp. nov.) and A2-67 ( sp. nov.) and the type strains of , and .

DNA–DNA relatedness between strains A2-50 ( sp. nov.) and A2-67 ( sp. nov.) and type strains of and .

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