1887

Abstract

A novel xylan-degrading bacterium, YT-1101, was isolated from fresh water. The isolate was a Gram-reaction-negative, aerobic, motile, endospore-forming and rod-shaped bacterium. Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain YT-1101 belonged to the genus , sharing sequence similarities of less than 94 % with the type species. The genomic G+C content was 58.6 mol%. The predominant menaquinone was MK-7. The major fatty acids were anteiso-C, iso-C and iso-C. Major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. On the basis of morphological, physiological and phylogenetic properties, strain YT-1101 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is YT-1101 (=NBRC 104957 =DSM 21753).

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2010-06-01
2020-01-19
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Micrographs of strain YT-1101 grown on TSA at 40 °C. (a, b) Scanning electron micrograph and phase-contrast micrograph showing peritrichous flagella. Bars, 1.0 µm (a) and 2.0 µm (b). (c, d) Transmission electron micrographs showing vegetative cells in cross and longitudinal section. CM, Cytoplasmic membrane; PG, peptidoglycan layer; S, S-layer. Bars, 0.2 µm (c) and 0.1 µm (d). (e, f) Transmission electron micrographs showing spores in cross and longitudinal section. Spike structures are indicated by arrowheads in (e). ES, Exosporium; SC, spore coat; CX, cortex; C, core. Bars, 0.2 µm.

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Two-dimensional TLC of polar lipids of strain YT-1101 . DPG, Diphosphatidylglycerol; PG, phosphatidylglycerol; PE, phosphatidylethanolamine; PL2, unknown phospholipid; PN1 and PN2, unknown aminophospholipids.

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