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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 60, Issue 4

sp. nov., isolated from traditional Korean fermented seafood Free

Abstract

A novel actinobacterium, strain P30, was isolated from jeotgal, a traditional Korean fermented seafood. Cells were aerobic, Gram-positive, non-motile and coccoid. Optimal growth occurred at 30–37 °C, at pH 8–9 and in the presence of 0–2 % (w/v) NaCl. Based on 16S rRNA gene sequence analysis, strain P30 was phylogenetically closely related to , , , , and with levels of similarity of 98.6, 98.2, 98.1, 97.4, 97.3 and 97.3 %, respectively, to the type strains of these species. Levels of DNA–DNA relatedness between strain P30 and the type strains of , , , and were 37, 43, 37, 25 and 17 %, respectively. The predominant menaquinone of strain P30 was MK-7. Major cellular fatty acids were anteiso-C, iso-C and iso-C. The genomic DNA G+C content of strain P30 was 70.2 mol%. Based on these data, strain P30 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is P30 (=KCTC 19594=JCM 15914).

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2010-04-01
2024-04-26
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References

  1. Chun J., Lee J. H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [CrossRef]
     [Google Scholar]
  2. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
     [Google Scholar]
  3. Gonzalez J. M., Saiz-Jimenez C. 2002; A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ Microbiol 4:770–773 [CrossRef]
     [Google Scholar]
  4. Hiraishi A., Ueda Y., Ishihara J., Mori T. 1996; Comparative lipoquinone analysis of influent sewage and activated sludge by high-performance liquid chromatography and photodiode array detection. J Gen Appl Microbiol 42:457–469 [CrossRef]
     [Google Scholar]
  5. Kim S. B., Nedashkovskaya O. I., Mikhailov V. V., Han S. K., Kim K. O., Rhee M. S., Bae K. S. 2004; Kocuria marina sp. nov., a novel actinobacterium isolated from marine sediment. Int J Syst Evol Microbiol 54:1617–1620 [CrossRef]
     [Google Scholar]
  6. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
     [Google Scholar]
  7. Kluge A. G., Farris J. S. 1969; Quantitative phyletics and the evolution of anurans. Syst Zool 18:1–32 [CrossRef]
     [Google Scholar]
  8. Kovacs G., Burghardt J., Pradella S., Schumann P., Stackebrandt E., Màrialigeti K. 1999; Kocuria palustris sp. nov. and Kocuria rhizophila sp. nov., isolated from the rhizoplane of the narrow-leaved cattail ( Typha angustifolia . Int J Syst Bacteriol 49:167–173 [CrossRef]
     [Google Scholar]
  9. Li W.-J., Zhang Y.-Q., Schumann P., Chen H.-H., Hozzein W. N., Tian X.-P., Xu L.-H., Jiang C.-L. 2006; Kocuria aegyptia sp. nov., a novel actinobacterium isolated from a saline, alkaline desert soil in Egypt. Int J Syst Evol Microbiol 56:733–737 [CrossRef]
     [Google Scholar]
  10. Mayilraj S., Kroppenstedt R. M., Suresh K., Saini H. S. 2006; Kocuria himachalensis sp. nov., an actinobacterium isolated from the Indian Himalayas. Int J Syst Evol Microbiol 56:1971–1975 [CrossRef]
     [Google Scholar]
  11. Park E. J., Roh S. W., Kim M. S., Jung M. J., Shin K. S., Bae J. W. 2010; Kocuria koreensis sp. nov. isolated from fermented seafood. Int J Syst Evol Microbiol 60:140–143 [CrossRef]
     [Google Scholar]
  12. Rainey F. A., Nobre M. F., Schumann P., Stackebrandt E., Da Costa M. S. 1997; Phylogenetic diversity of the deinococci as determined by 16S ribosomal DNA sequence comparison. Int J Syst Bacteriol 47:510–514 [CrossRef]
     [Google Scholar]
  13. Reddy G. S. N., Prakash J. S. S., Prabahar V., Matsumoto G. I., Stackebrandt E., Shivaji S. 2003; Kocuria polaris sp. nov., an orange-pigmented psychrophilic bacterium isolated from an Antarctic cyanobacterial mat sample. Int J Syst Evol Microbiol 53:183–187 [CrossRef]
     [Google Scholar]
  14. Roh S. W., Sung Y., Nam Y. D., Chang H. W., Kim K. H., Yoon J. H., Jeon C. O., Oh H. M., Bae J. W. 2008; Arthrobacter soli sp. nov., a novel bacterium isolated from wastewater reservoir sediment. J Microbiol 46:40–44 [CrossRef]
     [Google Scholar]
  15. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
     [Google Scholar]
  16. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual , 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
     [Google Scholar]
  17. Sasser M. 1990 Identification of bacteria by gas chromatography of cellular fatty acids , MIDI Technical Note 101 Newark, DE: MIDI Inc;
     [Google Scholar]
  18. Seo Y. B., Kim D. E., Kim G. D., Kim H. W., Nam S. W., Kim Y. T., Lee J. H. 2009; Kocuria gwangalliensis sp. nov., a novel actinobacterium isolated from seawater. Int J Syst Evol Microbiol 59:2769–2772 [CrossRef]
     [Google Scholar]
  19. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
     [Google Scholar]
  20. Stackebrandt E., Koch C., Gvozdiak O., Schumann P. 1995; Taxonomic dissection of the genus Micrococcus : Kocuria gen.nov., Nesterenkonia gen. nov., Kytococcus gen. nov., Dermacoccus gen. nov., and Micrococcus Cohn 1872 gen. emend. Int J Syst Bacteriol 45:682–692 [CrossRef]
     [Google Scholar]
  21. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega 4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [CrossRef]
     [Google Scholar]
  22. Tang S. K., Wang Y., Lou K., Mao P. H., Xu L. H., Jiang C. L., Kim C. J., Li W. J. 2009; Kocuria halotolerans sp. nov., a novel actinobacterium isolated from a saline soil in China. Int J Syst Evol Microbiol 59:1316–1320 [CrossRef]
     [Google Scholar]
  23. Tittsler R. P., Sandholzer L. A. 1936; Use of semi-solid agar for the detection of bacterial motility. J Bacteriol 31:575–580
     [Google Scholar]
  24. Tvrzová L., Schumann P., Sedlácek I., Pácová Z., Spröer C., Verbarg S., Kroppenstedt R. M. 2005; Reclassification of strain CCM 132, previously classified as Kocuria varians , as Kocuria carniphila sp. nov. Int J Syst Evol Microbiol 55:139–142 [CrossRef]
     [Google Scholar]
  25. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
     [Google Scholar]
  26. Zhou G., Luo X., Tang Y., Zhang L., Yang Q., Qio Y., Fang C. 2008; Kocuria flava sp. nov. and Kocuria turfanensis sp. nov., airborne actinobacteria isolated from Xinjiang, China. Int J Syst Evol Microbiol 58:1304–1307 [CrossRef]
     [Google Scholar]
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Kocuria atrinae sp. nov., isolated from traditional Korean fermented seafood
Int J Syst Evol Microbiol 60, 914 (2010); https://doi.org/10.1099/ijs.0.014506-0
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