1887

Abstract

A novel halophilic bacterium, designated strain MSS4, was isolated from the solar salterns of Mesolongi, Greece. The micro-organism, a motile, Gram-stain-positive, aerobic rod, proliferated at salinities of 1.0–4.0 M NaCl, with optimal growth at 2.5 M NaCl. Endospores were not observed. Strain MSS4 showed optimal growth at 37 °C and pH 8.0. The G+C content of its DNA was 47.2 mol%. The polar lipid pattern of strain MSS4 consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidic acid and phosphatidylethanolamine. It possessed anteiso-C, C, C and anteiso-C as the major fatty acids (altogether representing 84.7 % of the total). The predominant isoprenoid quinone was MK-7. The cell-wall peptidoglycan contained diaminopimelic acid. 16S rRNA gene sequence analysis showed that the new isolate has 96.1 % similarity to CM1 and 17-5, 95.5 % to BH169 and lower similarity to other species. These results justify the assignment of strain MSS4 to a novel species within the genus , for which the name sp. nov. is proposed. The type strain is MSS4 (=LMG 24571 =DSM 21373).

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2010-06-01
2019-10-22
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