1887

Abstract

Eleven strains that formed symbiotic root nodules on , designated previously as genospecies II, were identified as a new lineage of () that could be differentiated from all previously recognized species of the genus by using 16S rRNA gene sequences (<97.8 % similarity), DNA–DNA hybridization (<45 %), , , , , , and gene sequences, fatty acid profiles (C 7, 35 %;11-methyl C 7, 30 %) and numerical taxonomy. These strains are therefore considered to represent a novel species, for which the name sp. nov. is proposed, with isolate CCNWXJ12-2 (=ACCC 15461=HAMBI 3019) as the type strain.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.014043-0
2010-04-01
2020-09-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/4/958.html?itemId=/content/journal/ijsem/10.1099/ijs.0.014043-0&mimeType=html&fmt=ahah

References

  1. Chen W. X., Yan G. H., Li J. L. 1988; Numerical taxonomic study of fast-growing soybean rhizobia and proposal that Rhizobium fredii be assigned to Sinorhizobium gen. nov. Int J Syst Bacteriol 38:392–397 [CrossRef]
    [Google Scholar]
  2. Chen W. X., Wang E. T., Wang S., Li Y., Chen X., Li Y. 1995; Characterization of Rhizobium tianshanense sp. nov., a moderately and slow growing root nodule bacterium isolated from an arid saline environment in Xinjiang, People's Republic of China. Int J Syst Bacteriol 45:153–159 [CrossRef]
    [Google Scholar]
  3. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [CrossRef]
    [Google Scholar]
  4. Galtier N., Gouy M., Gautier C. 1996; seaview and phylo_win: two graphic tools for sequence alignment and molecular phylogeny. Comput Appl Biosci 12:543–548
    [Google Scholar]
  5. Gaunt M. W., Turner S. L., Rigottier-Gois L., Lioyd-Macgilp S. A., Young J. P. W. 2001; Phylogenies of atpD and recA s upport the small subunit rRNA-based classification of rhizobia. Int J Syst Evol Microbiol 51:2037–2048 [CrossRef]
    [Google Scholar]
  6. Ghosh W., Roy P. 2006; Mesorhizobium thiogangeticum sp. nov., a novel sulfur-oxidizing chemolithoautotroph from rhizosphere soil of an Indian tropical leguminous plant. Int J Syst Evol Microbiol 56:91–97 [CrossRef]
    [Google Scholar]
  7. Guindon S., Lethiec F., Duroux P., Gascuel O. 2005; phyml Online – a web server for fast maximum likelihood-based phylogenetic inference. Nucleic Acids Res 33:557–559 [CrossRef]
    [Google Scholar]
  8. Jarvis B. D. W., van Berkum P., Chen W. X., Nour S. M., Fernandez M. P., Cleyet-Marel J. C., Gillis M. 1997; Transfer of Rhizobium loti, Rhizobium huakuii, Rhizobium ciceri, Rhizobium mediterraneum , and Rhizobium tianshanense to Mesorhizobium gen. nov. Int J Syst Bacteriol 47:895–898 [CrossRef]
    [Google Scholar]
  9. Laguerre G., Nour S. M., Macheret V., Sanjuan J., Drouin P., Amarger N. 2001; Classification of rhizobia based on nodC and nifH gene analysis reveals a close phylogenetic relationship among Phaseolus vulgaris symbionts. Microbiology 147:981–993
    [Google Scholar]
  10. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [CrossRef]
    [Google Scholar]
  11. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [CrossRef]
    [Google Scholar]
  12. Peng G. X., Tan Z. Y., Wang E. T., Reinhold-Hurek B., Chen W. F., Chen W. X. 2002; Identification of isolates from soybean nodules in Xinjiang Region as Sinorhizobium xinjiangense and genetic differentiation of S. xinjiangense from S. fredii . Int J Syst Evol Microbiol 52:457–462
    [Google Scholar]
  13. Sneath P. H. A., Sokal R. B. 1973 Numerical Taxonomy. The Principles and Practice of Numerical Classification San Francisco: W. H. Freeman;
    [Google Scholar]
  14. Tan Z. Y., Kan F. L., Peng G. X., Wang E. T., Reinhold-Hurek B., Chen W. X. 2001; Rhizobium yanglingense sp. nov., isolated from arid and semi-arid regions in China. Int J Syst Evol Microbiol 51:909–914 [CrossRef]
    [Google Scholar]
  15. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  16. Tighe S. W., de Lajudie P., Dipietro K., Lindström K., Nick G., Jarvis B. D. W. 2000; Analysis of cellular fatty acids and phenotypic relationships of Agrobacterium, Bradyrhizobium, Mesorhizobium, Rhizobium and Sinorhizobium species using the Sherlock Microbial Identification System. Int J Syst Evol Microbiol 50:787–801 [CrossRef]
    [Google Scholar]
  17. Turner S. L., Young J. P. W. 2000; The glutamine synthetases of rhizobia: phylogenetics and evolutionary implications. Mol Biol Evol 17:309–319 [CrossRef]
    [Google Scholar]
  18. Wang E. T., van Berkum P., Sui X. H., Beyene D., Chen W. X., Martínez-Romero E. 1999; Diversity of rhizobia associated with Amorpha fruticosa isolated from Chinese soils and description of Mesorhizobium amorphae sp. nov. Int J Syst Bacteriol 49:51–65 [CrossRef]
    [Google Scholar]
  19. Wei G. H., Wang E. T., Tan Z. Y., Zhu M. E., Chen W. X. 2002; Rhizobium indigoferae sp. nov. and Sinorhizobium kummerowiae sp. nov., respectively isolated from Indigofera spp. and Kummerowia stipulaceae . Int J Syst Evol Microbiol 52:2231–2239 [CrossRef]
    [Google Scholar]
  20. Wei G. H., Tan Z. Y., Zhu M. E., Wang E. T., Han S. Z., Chen W. X. 2003; Characterization of rhizobia isolated from legume species within the genera Astragalus and Lespedeza grown in the Loess Plateau of China and description of Rhizobium loessense sp. nov. Int J Syst Evol Microbiol 53:1575–1583 [CrossRef]
    [Google Scholar]
  21. Wei G. H., Chen W. M., Young J. P. W., Bontemps C. 2009; A new clade of Mesorhizobium nodulating Alhagi sparsifolia . Syst Appl Microbiol 32:8–16 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.014043-0
Loading
/content/journal/ijsem/10.1099/ijs.0.014043-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Supplementary material 2

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error