1887

Abstract

An obligately anaerobic, Gram-positive, spore-forming bacterial strain, designated SL206, was isolated from pear orchard soils. Strain SL206 cells were straight or slightly curved rods, with motility by peritrichate flagella. Cell walls contained -diaminopimelic acid; wall sugars were glucose, rhamnose and mannose. The major fatty acids were C, C 9 and summed feature 10 (containing C 11/9/6). API 20A reactions were negative for oxidase, catalase and acid production from -rhamnose, sucrose, trehalose, -xylose, melezitose, salicin and -sorbitol, and positive for acid production from -glucose, sucrose, maltose, -mannose and raffinose. Glucose was fermented to acetate, butyrate, CO, H and ethanol in culture. The GC content of the genomic DNA was 31.1 mol%. Based on comparative 16S rRNA gene sequence analysis, the isolate belonged to the genus and formed a clade with . The species most closely related to strain SL206 were (98.6 % similarity) and (97.8 % similarity). In DNA–DNA relatedness studies, the isolate had 59.5 % relatedness with and thus represented a unique species. On the basis of these studies, strain SL206 (=KCTC 5449 =JCM 14858) is proposed to represent the type strain of a novel species, sp. nov.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.013953-0
2010-09-01
2020-09-22
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/9/2231.html?itemId=/content/journal/ijsem/10.1099/ijs.0.013953-0&mimeType=html&fmt=ahah

References

  1. Chang Y.-H., Han J., Chun J., Lee K. C., Rhee M.-S., Kim Y.-B., Bae K. S. 2002; Comamonas koreensis sp. nov., a non-motile species from wetland in Woopo, Korea. Int J Syst Evol Microbiol 52:377–381
    [Google Scholar]
  2. Chang Y.-H., Jung M. Y., Park I.-S., Oh H.-M. 2008; Sporolactobacillus vineae sp. nov., a spore-forming lactic acid bacterium isolated from vineyard soil. Int J Syst Evol Microbiol 58:2316–2320 [CrossRef]
    [Google Scholar]
  3. Collins M. D., Lawson P. A., Willems A., Cordoba J. J., Fernandez-Garayzabal J., Garcia P., Cai J., Hippe H., Farrow J. A. 1994; The phylogeny of the genus Clostridium : proposal of five new genera and eleven new species combinations. Int J Syst Bacteriol 44:812–826 [CrossRef]
    [Google Scholar]
  4. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  5. Felsenstein J. 1993 phylip (phylogeny inference package), version 3.5c. Distributed by the author. Department of Genome Sciences University of Washington; Seattle, USA:
    [Google Scholar]
  6. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [CrossRef]
    [Google Scholar]
  7. Fitch W. M., Margoliash E. 1967; Construction of phylogenetic trees. Science 155:279–284 [CrossRef]
    [Google Scholar]
  8. Heyndrickx M., De Vos P., De Ley J. 1991; Fermentation characteristics of Clostridium pasteurianum LMG 3285 grown on glucose and mannitol. J Appl Bacteriol 70:52–58 [CrossRef]
    [Google Scholar]
  9. Holdeman L. V., Cato E. P., Moore W. E. C. 1977 Anaerobe Laboratory Manual, 4th edn. Blacksburg, VA: Virginia Polytechnic Institute and State University;
    [Google Scholar]
  10. Jeong H., Yi H., Sekiguchi Y., Muramatsu M., Kamagata Y., Chun J. 2004; Clostridium jejuense sp. nov., isolated from soil. Int J Syst Evol Microbiol 54:1465–1468 [CrossRef]
    [Google Scholar]
  11. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism vol 3 pp 21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  12. Kasap M., Chen J.-S. 2005; Clostridium pasteurianum W5 synthesizes two NifH-related polypeptides under nitrogen-fixing conditions. Microbiology 151:2353–2362 [CrossRef]
    [Google Scholar]
  13. Kim S., Jeong H., Chun J. 2006; Clostridium ganghwense sp. nov., isolated from tidal flat sediment. Int J Syst Evol Microbiol 56:691–693 [CrossRef]
    [Google Scholar]
  14. Kim S., Jeong H., Chun J. 2007; Clostridium aestuarii sp. nov., from tidal flat sediment. Int J Syst Evol Microbiol 57:1315–1317 [CrossRef]
    [Google Scholar]
  15. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207
    [Google Scholar]
  16. Kuhner C. H., Matthies C., Acker G., Schmittroth M., Gößner A. S., Drake H. L. 2000; Clostridium akagii sp. nov. and Clostridium acidisoli sp. nov.: acid-tolerant, N2-fixing clostridia isolated from acidic forest soil and litter. Int J Syst Evol Microbiol 50:873–881 [CrossRef]
    [Google Scholar]
  17. Lee I.-H., Park J. Y., Kho D. H., Kim M.-S., Lee J. K. 2002; Reductive effect of H2 uptake and poly- β -hydroxybutyrate formation on nitrogenase-mediated H2 accumulation of Rhodobacter sphaeroides according to light intensity. Appl Microbiol Biotechnol 60:147–153 [CrossRef]
    [Google Scholar]
  18. Lee Y.-J., Romanek C. S., Wiegel J. 2007; Clostridium aciditolerans sp. nov., an acid-tolerant spore-forming anaerobic bacterium from constructed wetland sediment. Int J Syst Evol Microbiol 57:311–315 [CrossRef]
    [Google Scholar]
  19. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  20. Steer T., Collins M. D., Gibson G. R., Hippe H., Lawson P. A. 2001; Clostridium hathewayi sp. nov., from human faeces. Syst Appl Microbiol 24:353–357 [CrossRef]
    [Google Scholar]
  21. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
    [Google Scholar]
  22. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  23. Wiegel J., Tanner R., Rainey F. A. 2006; An introduction to the family Clostridiaceae . In The Prokaryotes: a Handbook on the Biology of Bacteria, 3rd edn. vol 4 pp 654–678 Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E. New York: Springer;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.013953-0
Loading
/content/journal/ijsem/10.1099/ijs.0.013953-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error