1887

Abstract

A thermophilic, anaerobic, chemosynthetic bacterium, designated strain MB-1, was isolated from the walls of an active deep-sea hydrothermal vent chimney on the East Pacific Rise at  ° 50′ N 10 ° 17′ W. The cells were Gram-negative-staining rods, approximately 1–1.5 μm long and 0.3–0.5 μm wide. Strain MB-1 grew at 25–65 °C (optimum 55 °C), with 10–35 g NaCl l (optimum 20 g l) and at pH 4.5–8.5 (optimum pH 7.0). Generation time under optimal conditions was 45.6 min. Growth occurred under chemolithoautotrophic conditions with H as the energy source and CO as the carbon source. Nitrate was used as the electron acceptor, with resulting production of ammonium. Thiosulfate, sulfur and selenate were also used as electron acceptors. No growth was observed in the presence of lactate, peptone or tryptone. Chemo-organotrophic growth occurred in the presence of acetate, formate, Casamino acids, sucrose, galactose and yeast extract under a N/CO gas phase. The G+C content of the genomic DNA was 36.0 mol%. Phylogenetic analysis of the 16S rRNA gene sequence indicated that this organism is closely related to AmH, PH1209 and 525 (95, 94 and 93 % sequence identity, respectively). On the basis of phylogenetic, physiological and genetic considerations, it is proposed that the organism represents a novel species within the genus , sp. nov. The type strain is MB-1 (=DSM 22087 =JCM 15746).

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2010-05-01
2019-10-21
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References

  1. Alain, K., Querellou, J., Lesongeur, F., Pignet, P., Crassous, P., Raguénès, G., Cueff, V. & Cambon-Bonavita, M.-A. ( 2002; ). Caminibacter hydrogeniphilus gen. nov., sp nov., a novel thermophilic, hydrogen-oxidizing bacterium isolated from an East Pacific Rise hydrothermal vent. Int J Syst Evol Microbiol 52, 1317–1323.[CrossRef]
    [Google Scholar]
  2. Alain, K., Callac, N., Guégan, M., Lesongeur, F., Crassous, P., Cambon-Bonavita, M.-A., Querellou, J. & Prieur, D. ( 2009; ). Nautilia abyssi sp. nov., a thermophilic, chemolithoautotrophic, sulfur-reducing bacterium isolated from an East Pacific Rise hydrothermal vent. Int J Syst Evol Microbiol 59, 1310–1315.[CrossRef]
    [Google Scholar]
  3. Campbell, B. J., Engel, A. S., Porter, M. L. & Takai, K. ( 2006; ). The versatile epsilon-proteobacteria: key players in sulphidic habitats. Nat Rev Microbiol 4, 458–468.[CrossRef]
    [Google Scholar]
  4. DeSoete, G. ( 1983; ). A least squares algorithm for fitting additive trees to proximity data. Psychometrika 48, 621–626.[CrossRef]
    [Google Scholar]
  5. Diamond, D. ( 1993a; ). Nitrate in brackish or seawater. In QuickChem Automated Ion Analyzer Methods Manual, method 31-107-04-1-A. Milwaukee, WI: Lachat Instruments.
  6. Diamond, D. ( 1993b; ). Ammonia in brackish or seawater. In QuickChem Automated Ion Analyzer Methods Manual, method 31-107-06-1-A. Milwaukee, WI: Lachat Instruments.
  7. Galtier, N., Gouy, M. & Gautier, C. ( 1996; ). seaview and phylo_win: two graphic tools for sequence alignment and molecular phylogeny. Comput Appl Biosci 12, 543–548.
    [Google Scholar]
  8. Garrity, G. M., Bell, J. A. & Lilburn, T. ( 2005; ). Order I. Campylobacterales ord. nov. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 2, part C, p. 1145. Edited by D. J. Brenner, N. R. Krieg, J. T. Staley & G. M. Garrity. New York: Springer.
  9. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  10. Miroshnichenko, M. L., Kostrikina, N. A., L'Haridon, S., Jeanthon, C., Hippe, H., Stackebrandt, E. & Bonch-Osmolovskaya, E. A. ( 2002; ). Nautilia lithotrophica gen. nov., sp nov., a thermophilic sulfur-reducing ϵ-proteobacterium isolated from a deep-sea hydrothermal vent. Int J Syst Evol Microbiol 52, 1299–1304.[CrossRef]
    [Google Scholar]
  11. Miroshnichenko, M. L., L'Haridon, S., Schumann, P., Spring, S., Bonch-Osmolovskaya, E. A., Jeanthon, C. & Stackebrandt, E. ( 2004; ). Caminibacter profundus sp. nov., a novel thermophile of Nautiliales ord. nov. within the class ‘Epsilonproteobacteria’, isolated from a deep-sea hydrothermal vent. Int J Syst Evol Microbiol 54, 41–45.[CrossRef]
    [Google Scholar]
  12. Perrière, G. & Gouy, M. ( 1996; ). WWW-Query: an on-line retrieval system for biological sequence banks. Biochimie 78, 364–369.[CrossRef]
    [Google Scholar]
  13. Smith, J. L., Campbell, B. J., Hanson, T. E., Zhang, C. L. & Cary, S. C. ( 2008; ). Nautilia profundicola sp. nov., a thermophilic, sulfur-reducing epsilonproteobacterium from deep-sea hydrothermal vents. Int J Syst Evol Microbiol 58, 1598–1602.[CrossRef]
    [Google Scholar]
  14. Stetter, K. O., König, H. & Stackebrandt, E. ( 1983; ). Pyrodictium gen. nov., a new genus of submarine disc-shaped sulfur-reducing archaebacteria growing optimally at 105 °C. Syst Appl Microbiol 4, 535–551.[CrossRef]
    [Google Scholar]
  15. Takai, K., Hirayama, H., Nakagawa, T., Suzuki, Y., Nealson, K. H. & Horikoshi, K. ( 2005; ). Lebetimonas acidiphila gen. nov., sp. nov., a novel thermophilic, acidophilic, hydrogen-oxidizing chemolithoautotroph within the ‘Epsilonproteobacteria’, isolated from a deep-sea hydrothermal fumarole in the Mariana Arc. Int J Syst Evol Microbiol 55, 183–189.[CrossRef]
    [Google Scholar]
  16. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x Windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  17. Vetriani, C., Jannasch, H. W., MacGregor, B. J., Stahl, D. A. & Reysenbach, A. L. ( 1999; ). Population structure and phylogenetic characterization of marine benthic archaea in deep-sea sediments. Appl Environ Microbiol 65, 4375–4384.
    [Google Scholar]
  18. Vetriani, C., Speck, M. D., Ellor, S. V., Lutz, R. A. & Starovoytov, V. ( 2004; ). Thermovibrio ammonificans sp. nov., a thermophilic, chemolithotrophic, nitrate-ammonifying bacterium from deep-sea hydrothermal vents. Int J Syst Evol Microbiol 54, 175–181.[CrossRef]
    [Google Scholar]
  19. Voordeckers, J. W., Starovoytov, V. & Vetriani, C. ( 2005; ). Caminibacter mediatlanticus sp. nov., a thermophilic, chemolithoautotrophic, nitrate-ammonifying bacterium isolated from a deep-sea hydrothermal vent on the Mid-Atlantic Ridge. Int J Syst Evol Microbiol 55, 773–779.[CrossRef]
    [Google Scholar]
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vol. , part 5, pp. 1182 - 1186

Generation times of sp. nov. MB-1 at different temperatures, NaCl concentrations and pH.

Nitrate consumption and ammonium formation during growth of sp. nov. MB-1 .

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