1887

Abstract

A moderately thermophilic bacterial strain (HT4) isolated from a hot spring sediment was characterized phenotypically and phylogenetically. Cells were Gram-negative, aerobic, non-sporulating, rod-shaped and motile by means of a single polar flagellum. Both oxidase and catalase activities were positive. Heterotrophic growth was observed at pH 6.0–8.5 and at 20–50 °C; optimum growth occurred at pH 7.5–8.0 and 37–42 °C. The major cellular fatty acids were C 3-OH, C 3-OH, C 2-OH, C 7 and C cyclo 8. The DNA G+C content of strain HT4 was 67.8 mol%.16S rRNA gene sequence analysis indicated that strain HT4 clustered within the radiation of the genus and showed 99.0 % similarity with CCUG 54519 and 96 % similarity with DSM 6462. However, levels of DNA–DNA relatedness between strain HT4 and CCUG 54519 and DSM 6462 were 52 and 20 %, respectively. On the basis of the phenotypic, physiological and chemotaxonomic data, 16S rRNA gene sequence analysis and DNA–DNA hybridization results, strain HT4 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is HT4 (=DSM 18167=JCM 14988).

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2010-04-01
2020-09-23
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