1887

Abstract

A moderately thermophilic bacterial strain (HT4) isolated from a hot spring sediment was characterized phenotypically and phylogenetically. Cells were Gram-negative, aerobic, non-sporulating, rod-shaped and motile by means of a single polar flagellum. Both oxidase and catalase activities were positive. Heterotrophic growth was observed at pH 6.0–8.5 and at 20–50 °C; optimum growth occurred at pH 7.5–8.0 and 37–42 °C. The major cellular fatty acids were C 3-OH, C 3-OH, C 2-OH, C 7 and C cyclo 8. The DNA G+C content of strain HT4 was 67.8 mol%. 16S rRNA gene sequence analysis indicated that strain HT4 clustered within the radiation of the genus and showed 99.0 % similarity with CCUG 54519 and 96 % similarity with DSM 6462. However, levels of DNA–DNA relatedness between strain HT4 and CCUG 54519 and DSM 6462 were 52 and 20 %, respectively. On the basis of the phenotypic, physiological and chemotaxonomic data, 16S rRNA gene sequence analysis and DNA–DNA hybridization results, strain HT4 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is HT4 (=DSM 18167=JCM 14988).

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2010-04-01
2019-10-24
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References

  1. Alarico, S., Rainey, F. A., Empadinhas, N., Schumann, P., Nobre,M. F. & da Costa, M. S. ( 2002; ). Rubritepidaflocculans gen. nov., sp. nov., a new slightly thermophilic member ofthe alpha-1 subclass of the Proteobacteria. Syst Appl Microbiol 25, 198–206.[CrossRef]
    [Google Scholar]
  2. Berglund, F. & Sorbo, B. H. ( 1960; ). Turbidimetric analysis of inorganic sulfate in serum, plasma and urine. Scand J Clin Lab Invest 12, 147–153.[CrossRef]
    [Google Scholar]
  3. Das, S. K., Mishra, A. K., Tindall, B. J., Rainey, F. A. &Stackebrandt, E. ( 1996; ). Oxidation of thiosulfateby a new bacterium, Bosea thiooxidans (strain BI-42) gen.nov., sp. nov.: analysis of phylogeny based on chemotaxonomy and 16S ribosomalDNA sequencing. Int J Syst Bacteriol 46, 981–987.[CrossRef]
    [Google Scholar]
  4. Denner, E. M. B., Kolari, M., Hoornstra, D., Tsitko, I., Kämpfer,P., Busse, H.-J. & Salkinoja-Salonen, M. ( 2006; ). Rubellimicrobium thermophilum gen. nov., sp. nov., a red-pigmented, moderatelythermophilic bacterium isolated from coloured slime deposits in paper machines. Int J Syst Evol Microbiol 56, 1355–1362.[CrossRef]
    [Google Scholar]
  5. Donachie, S. P., Bowman, J. P. & Alam, M. ( 2006; ). Nesiotobacter exalbescens gen. nov., sp. nov.,a moderately thermophilic alphaproteobacterium from an Hawaiian hypersalinelake. Int J Syst Evol Microbiol 56, 563–567.[CrossRef]
    [Google Scholar]
  6. Egli, T. W. & Auling, G. ( 2005; ).Genus Chelatococcus. In Bergey's Manual of Systematic Bacteriology, Part C, vol. 2, 2nd edn, pp. 433–437. Edited by D. J. Brenner,N. R. Krieg & J. T. Stanley. New York: Springer.
  7. Egli, T. W., Weilenmann, H.-U., El-Banna, T. & Auling, G. ( 1988; ). Gram-negative, aerobic, nitrilotriacetate-utilizingbacteria from wastewater and soil. Syst Appl Microbiol 10, 297–305.[CrossRef]
    [Google Scholar]
  8. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridizationin microdilution wells as an alternative to membrane filter hybridizationin which radioisotopes are used to determine genetic relatedness among bacterialstrains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  9. Felsenstein, J. ( 1985; ). Confidence limitson phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  10. Ghosh, D., Bal, B., Kashyap, V. K. & Pal, S. ( 2003; ). Molecular phylogenetic exploration of bacterial diversityin a Bakreshwar (India) hot spring and culture of Shewanella-related thermophiles. Appl Environ Microbiol 69, 4332–4336.[CrossRef]
    [Google Scholar]
  11. Gleen, H. & Quastel, J. H. ( 1952; ).Sulfur metabolism in soil. Appl Microbiol 1, 70–77.
    [Google Scholar]
  12. Gupta, M. L., Narain, H. & Saxena, V. K. ( 1975; ). Geochemistry of thermal waters from various geothermal provincesof India. In Proceedings of the Grenoble Symposium of the InternationalAssociation of Hydrological Sciences, pp. 47–58. Wallingford, UK:IAHS Press, Institute of Hydrology.
  13. Johnson, D. B., Stallwood, B., Kimura, S. & Hallberg, K.B. ( 2006; ). Isolation and characterization of Acidicaldus organivorus, gen. nov., sp. nov.: a novel sulfur-oxidizing,ferric iron-reducing thermo-acidophilic heterotrophic Proteobacterium. Arch Microbiol 185, 212–221.[CrossRef]
    [Google Scholar]
  14. Jukes, T. H. & Cantor, C. R. ( 1969; ). Evolution of protein molecules. In Mammalian Protein Metabolism,vol. 3, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  15. Kanso, S. & Patel, B. K. ( 2004; ). Phenylobacterium lituiforme sp. nov., a moderately thermophilic bacteriumfrom a subsurface aquifer, and emended description of the genus Phenylobacterium. Int J Syst Evol Microbiol 54, 2141–2146.[CrossRef]
    [Google Scholar]
  16. Kelly, D. P., Chambers, L. A. & Trudinger, P. A. ( 1969; ). Cyanolysis and spectrophotometric estimation of trithionatein mixture with thiosulfate and tetrathionate. Anal Chem 41, 898–901.[CrossRef]
    [Google Scholar]
  17. Kuykendall, L. D., Roy, M. D., O’Neill, J. J. & Devine,T. E. ( 1988; ). Fatty acids, antibiotic resistance anddeoxyribonucleic acid homology groups of Bradyrhizobium japonicum. Int J Syst Bacteriol 38, 358–361.[CrossRef]
    [Google Scholar]
  18. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleicacid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  19. Panda, S. K., Jyoti, V., Bhadra, B., Nayak, K. C., Shivaji,S., Rainey, F. A. & Das, S. K. ( 2009; ). Thiomonasbhubaneswarensis sp. nov., an obligately mixotrophic, moderately thermophilic,thiosulfate-oxidizing bacterium. Int J Syst Evol Microbiol 59, 2171–2175.[CrossRef]
    [Google Scholar]
  20. Rainey, F. A., Silva, J., Nobre, M. F., Silva, M. T. & daCosta, M. S. ( 2003; ). Porphyrobacter cryptussp. nov., a novel slightly thermophilic, aerobic, bacteriochlorophyll a-containing species. Int J Syst Evol Microbiol 53, 35–41.[CrossRef]
    [Google Scholar]
  21. Sharma, D. P., Thomas, C., Hall, R. H., Levine, M. M. &Attridge, S. R. ( 1989; ). Significance of toxin coregulatedpili as protective antigens of Vibrio cholerae in the infant mousemodel. Vaccine 7, 451–456.[CrossRef]
    [Google Scholar]
  22. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequenceanalysis in the present species definition in bacteriology. IntJ Syst Bacteriol 44, 846–849.
    [Google Scholar]
  23. Tamaoka, J. & Komagata, K. ( 1984; ).Determination of DNA base composition by reversed-phase high-performance liquidchromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef]
    [Google Scholar]
  24. Tamura, K., Dudley, J., Nei, M. & Kumar, S. ( 2007; ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24, 1596–1599.[CrossRef]
    [Google Scholar]
  25. Wayne, L. G., Good, R. C., Krichevsky, M. I., Blacklock, Z.,David, H. L., Dawson, D., Gross, W., Hawkins, J., Levy-Frebault, V. V. &other authors ( 1991; ). Fourth report of the cooperative,open-ended study of slowly growing mycobacteria by the International WorkingGroup on Mycobacterial Taxonomy. Int J Syst Bacteriol 41, 463–472.[CrossRef]
    [Google Scholar]
  26. Yoon, J. H., Kang, S. J., Im, W. T., Lee, S. T. & Oh, T.K. ( 2008; ). Chelatococcus daeguensis sp. nov.,isolated from wastewater of a textile dye works, and emended description ofthe genus Chelatococcus. Int J Syst Evol Microbiol 58, 2224–2228.[CrossRef]
    [Google Scholar]
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vol. , part 4, pp. 861 - 865

Fatty acid composition of strain HT4 , CCUG 54519 and DSM 6462 . [ PDF] 56 KB



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