1887

Abstract

Phylogenetic analyses of type and reference strains of biogroups 1 and 2 plus a novel isolate of biogroup 2 were carried out based on 16S rRNA gene sequences and partial sequences of four protein-coding genes (, , and ). Both approaches revealed that biogroup 1 strains were closely related to . Biogroup 2 strains, however, formed a distinct monophyletic clade of generic status that included . Phenotypic tests were consistent with the molecular classification and provided diagnostic features. It is proposed that biogroup 2 strains be placed in a new genus, gen. nov., as sp. nov., with strain 521 (=DSM 3038=LMG 24835=NCIMB 14534) as the type strain, and that be transferred to the genus as comb. nov., with strain ATCC 29907 (=DSM 4481) as the type strain.

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2010-04-01
2020-09-22
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References

  1. Brenner D. J. 1981; Introduction to the family Enterobacteriaceae . In The Prokaryotes pp 1105–1127 Edited by Starr M. P., Stolp H., Trüper H., Schlegel H. G. Berlin: Springer-Verlag;
    [Google Scholar]
  2. Burgess N. R. H., McDermott S. N., Whiting J. 1973; Anaerobic bacteria occurring in the hind-gut of the cockroach, Blatta orientalis . J Hyg Camb 71:1–6 [CrossRef]
    [Google Scholar]
  3. Case R. J., Boucher Y., Dahllöf I., Holmström C., Doolittle W. F., Kjelleberg S. 2007; Use of 16S rRNA and rpoB genes as molecular markers for microbial ecology studies. Appl Environ Microbiol 73:278–288 [CrossRef]
    [Google Scholar]
  4. Charrier M., Fonty G., Gaillard-Martinie B., Ainouche K., Andant G. 2006; Isolation and characterization of cultivable fermentative bacteria from the intestine of two edible snails, Helix pomatia and Cornu aspersum (Gastropoda: Pulmonata). Biol Res 39:669–681
    [Google Scholar]
  5. Embley T. M. 1991; The linear PCR reaction: a simple and robust method for sequencing amplified rRNA genes. Lett Appl Microbiol 13:171–174 [CrossRef]
    [Google Scholar]
  6. Farmer J. J., Brenner D. J. 2005; Genus XXII. Obesumbacterium Shimwell 1963, 759AL . In Bergey's Manual of Systematic Bacteriology , The Proteobacteria , Part B: The Gammaproteobacteria ,, 2nd edn. vol 2 pp 710–713 Edited by Garrity G., Brenner D. J., Krieg N. R., Staley J. R. New York: Springer;
    [Google Scholar]
  7. Janda J. M., Abbott S. L. 2006; The genus Hafnia : from soup to nuts. Clin Microbiol Rev 19:12–18
    [Google Scholar]
  8. Koivula T. T., Juvonen R., Haikara A., Suihko M. L. 2006; Characterization of the brewery spoilage bacterium Obesumbacterium proteus by automated ribotyping and development of PCR methods for its biotype 1. J Appl Microbiol 100:398–406 [CrossRef]
    [Google Scholar]
  9. Maugueret T. M., Walker S. L. 2002; Rapid detection of Obesumbacterium proteus from yeast and wort using polymerase chain reaction. Lett Appl Microbiol 35:281–284 [CrossRef]
    [Google Scholar]
  10. Page R. D. M. 1996; TreeView: An application to display phylogenetic trees on personal computers. Comput Appl Biosci 12:357–358
    [Google Scholar]
  11. Prest A. G., Hammond J. R., Stewart G. S. 1994; Biochemical and molecular characterization of Obesumbacterium proteus , a common contaminant of brewing yeasts. Appl Environ Microbiol 60:1635–1640
    [Google Scholar]
  12. Priest F. G., Somerville H. J., Cole J. A., Hough J. S. 1973; The taxonomic position of Obesumbacterium proteus , a common brewery contaminant. J Gen Microbiol 75:295–307 [CrossRef]
    [Google Scholar]
  13. Salerno A., Delétoile A., Lefevre M., Ciznar I., Krovacek K., Grimont P., Brisse S. 2007; Recombining population structure of Plesiomonas shigelloides ( Enterobacteriaceae ) revealed by multilocus sequence typing. J Bacteriol 189:7808–7818 [CrossRef]
    [Google Scholar]
  14. Scheutz F., Strockbine N. A. 2005; Genus I. Escherichia Castellani and Chalmers 1919, 941AL . In Bergey's Manual of Systematic Bacteriology , The Proteobacteria , Part B: The Gammaproteobacteria , 2nd edn. vol 2 pp 607–625 Edited by Garrity G., Brenner D. J., Krieg N. R., Staley J. R. New York: Springer;
    [Google Scholar]
  15. Shimwell J. L. 1936; A study of the common rod bacteria of brewers’ yeast. J Inst Brew 42:119–127 [CrossRef]
    [Google Scholar]
  16. Shimwell J. L. 1963; Obesumbacterium gen. nov. Brew J 49:759–760
    [Google Scholar]
  17. Shimwell J. L. 1964; Obesumbacterium , a new genus for the inclusion of “ Flavobacterium proteus ”. J Inst Brew 70:247–248 [CrossRef]
    [Google Scholar]
  18. Shimwell J. L., Grimes M. 1936; The distinguishing characters of Flavobacterium proteus (sp. nov.), the common rod bacterium of brewers’ yeast. J Inst Brew 42:348–350 [CrossRef]
    [Google Scholar]
  19. Skrodenyte-Arbaciauskiene V., Sruoga A., Butkauskas D. 2006; Assessment of microbial diversity in the river trout Salmo trutta fario L. intestinal tract identified by partial 16S rRNA gene sequence analysis. Fish Sci 72:597–602 [CrossRef]
    [Google Scholar]
  20. Strandskov F. B., Baker H. W., Bockelmann J. B. 1953; A study of the gram-negative bacterial rod infection of brewery yeast and brewery fermentations. Wallerstein Lab Commun 16:261–270
    [Google Scholar]
  21. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: Molecular Evolutionary Genetics Analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [CrossRef]
    [Google Scholar]
  22. Van Vuuren H. J. J., Priest F. G. 2003; Gram-negative brewery bacteria. In Brewing Microbiology pp 219–245 Edited by Priest F. G., Campbell I. New York: Kluwer Academic/Plenum Publishers;
    [Google Scholar]
  23. van Vuuren H. J., Kersters K., De Ley J., Toerien D. F. 1981; The identification of Enterobacteriaceae from breweries: combined use and comparison of API 20E system, gel electrophoresis of proteins and gas chromatography of volatile metabolites. J Appl Bacteriol 51:51–65
    [Google Scholar]
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