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Abstract

A novel aerobic, Gram-negative, motile, rod-shaped bacterial strain designated 5516S-2 was isolated from an air sample taken in Suwon, Republic of Korea. Colonies were yellow-pigmented and circular with entire margins. 16S rRNA gene sequence analysis showed that strain 5516S-2 was closely related to DSM 7250 (97.6 % sequence similarity), KACC 11579 (97.5 %) and DSM 13620 (97.1 %). However, the phylogenetic tree indicated that strain 5516S-2 formed a separate clade from . Strain 5516S-2 displayed 42, 31 and 30 % DNA–DNA relatedness to the type strains of , and , respectively. The major fatty acids (>10 % of total fatty acids) were C (33.3 %), C cyclo (18.8 %), C 7 (17.5 %) and summed feature 3 (comprising C 7 and/or iso-C 2-OH; 13.0 %). The DNA G+C content was 69 mol%. The major quinone was ubiquinone Q-8. The predominant polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and two unknown aminophospholipids. Genotypic and phenotypic characteristics clearly distinguished strain 5516S-2 from closely related species and indicated that it represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is 5516S-2 (=KACC 12602=DSM 19424).

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2010-02-01
2020-01-23
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Polar lipid profiles of strain 5516S-2 ( sp. nov.) and related strains. [PDF](45 KB)

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Maximum-parsimony tree based on almost-complete 16S rRNA gene sequences, showing the phylogenetic position of strain 5516S-2 ( sp. nov.). [PDF](26 KB)

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Cellular fatty acid compositions of strain 5516S-2 ( sp. nov.) and related strains. [PDF](47 KB)

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