A novel actinomycete, designated strain CS5-AC17, was isolated from cliff soil in the Republic of Korea. Cells of the organism were aerobic and catalase- and oxidase-negative. Substrate mycelium was well developed and was pale to strong yellow. Spore chains were borne on rudimentary aerial mycelium. The spores (0.4–0.5×0.9–1.6 μm) were smooth surfaced and cylindrical. Growth occurred at 25–42 °C, at pH 5.1–12.1 and in the presence of up to 4 % NaCl. Phylogenetic analyses based on 16S rRNA gene sequences indicated that the isolate was closely related to members of the genus in the family . The phylogenetic neighbours were TT2-10 (97.7 % 16S rRNA gene sequence similarity) and KV-744 (97.6 %). The diagnostic diamino acid in the cell-wall peptidoglycan was -diaminopimelic acid. The major respiratory quinones were MK-9(H), MK-9(H) and MK-9(H). The polar lipids contained phosphatidylethanolamine and phosphatidylinositol with a small amount of phosphatidylglycerol. The DNA G+C content was 74.3 mol%. DNA–DNA relatedness values between strain CS5-AC17 and its phylogenetically closest relatives were 6.1–7.1 % ( DSM 44816) and 21.5–27.6 % ( NRRL B-24477). On the basis of the phenotypic features and the DNA–DNA hybridization data, strain CS5-AC17 (=DSM 45178=NRRL B-24660) represents a novel species of the genus , for which the name sp. nov. is proposed.


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Scanning electron micrographs of growth of strain CS5-AC17 after 3–4 weeks at 30 °C. (a) Rudimentary aerial mycelium (oatmeal-nitrate agar); (b) spore chains (oatmeal-nitrate agar); (c) swelling of hyphal tips of vegetative mycelium (ISP 5). Bars, 1 µm.


Scanning electron micrographs of growth of strain CS5-AC17 after 3–4 weeks at 30 °C. (a) Rudimentary aerial mycelium (oatmeal-nitrate agar); (b) spore chains (oatmeal-nitrate agar); (c) swelling of hyphal tips of vegetative mycelium (ISP 5). Bars, 1 µm.


Scanning electron micrographs of growth of strain CS5-AC17 after 3–4 weeks at 30 °C. (a) Rudimentary aerial mycelium (oatmeal-nitrate agar); (b) spore chains (oatmeal-nitrate agar); (c) swelling of hyphal tips of vegetative mycelium (ISP 5). Bars, 1 µm.


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