1887

Abstract

A Gram-positive-staining, non-motile, rod- or coccoid-shaped bacterium, strain CLG, was isolated from beach sand from Lianyungang, Jiangsu Province, PR China. The organism grew optimally at 30 °C and pH 7.0. Strain CLG contained -Lys–-Asp as the peptidoglycan type and galactose, rhamnose and xylose as the whole-cell sugars. It contained anteiso-C and iso-C as the major fatty acids. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phospholipid, phosphatidylinositol, three phosphoglycolipids and one unidentified lipid. The DNA G+C content was 70.3 mol%. The 16S rRNA gene sequence similarity of strain CLG with DS-3 was 99.4 % but the DNA–DNA relatedness between strain CLG and DS-3 was 58.7 %. There were also many phenotypic properties that distinguished strain CLG from recognized species of the genus . The results showed that strain CLG represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CLG (=DSM 21863=CCTCC AB208287).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.012864-0
2010-04-01
2021-04-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/4/904.html?itemId=/content/journal/ijsem/10.1099/ijs.0.012864-0&mimeType=html&fmt=ahah

References

  1. Ausubel F.-M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. (editors) 1999; Short Protocols in Molecular Biology . , 4th edn. New York: Wiley;
  2. Bakalidou A., Kämpfer P., Berchtold M., Kuhnigk T., Wenzel M., König H. 2002; Cellulosimicrobium variabile sp. nov., a cellulolytic bacterium from the hindgut of the termite Mastotermes darwiniensis . Int J Syst Evol Microbiol 52:1185–1192 [CrossRef]
    [Google Scholar]
  3. De Ley J. 1970; Reexamination of the association between melting point, buoyant density, and chemical base composition of DNA. J Bacteriol 101:738–754
    [Google Scholar]
  4. De Ley J., Cattoir H., Reynaeerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [CrossRef]
    [Google Scholar]
  5. Groth I., Schumann P., Schütze B., Gonzalez J.-M., Laiz L., Cesareo S.-J., Stackebrandt E. 2005; Isoptericola hypogeus sp. nov., isolated from the Roman catacomb of Domitilla. Int J Syst Evol Microbiol 55:1715–1719 [CrossRef]
    [Google Scholar]
  6. Lányí B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67
    [Google Scholar]
  7. MacKenzie S. L. 1987; Gas chromatographic analysis of amino acids as the N -heptafluorobutyryl isobutyl esters. J Assoc Off Anal Chem 70:151–160
    [Google Scholar]
  8. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [CrossRef]
    [Google Scholar]
  9. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  10. Schleifer K. H. 1985; Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18:123–156
    [Google Scholar]
  11. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477
    [Google Scholar]
  12. Stackebrandt E., Schumann P., Cui X.-L. 2004; Reclassification of Cellulosimicrobium variabile Bakalidou et al. 2002 as Isoptericola variabilis gen. nov., comb. nov. Int J Syst Evol Microbiol 54:685–688 [CrossRef]
    [Google Scholar]
  13. Staneck J. L., Roberts G. D. 1974; Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28:226–231
    [Google Scholar]
  14. Whiton R. S., Lau P., Morgan S. L., Gilbart J., Fox A. 1985; Modifications in the alditol acetate method for analysis of muramic acid and other neutral and amino sugars by capillary gas chromatography-mass spectrometry with selected ion monitoring. J Chromatogr 347:109–120 [CrossRef]
    [Google Scholar]
  15. Yoon J.-H., Schumann P., Kang S.-J., Jung S.-Y., Oh T.-K. 2006; Isoptericola dokdonensis sp. nov., isolated from soil. Int J Syst Evol Microbiol 56:2893–2897 [CrossRef]
    [Google Scholar]
  16. Zhang Y.-Q., Schumann P., Li W.-J., Chen G.-Z., Tian X.-P., Stackebrandt E., Xu L.-H., Jiang C.-L. 2005; Isoptericola halotolerans sp. nov., a novel actinobacterium isolated from saline soil from Qinghai Province, north-west China. Int J Syst Evol Microbiol 55:1867–1870 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.012864-0
Loading
/content/journal/ijsem/10.1099/ijs.0.012864-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error