1887

Abstract

Twenty coagulase-negative strains displaying -haemolysis (-haemolysin) on sheep-blood agar were isolated from the noses of different pigs in Switzerland. The strains were Gram-stain-positive, non-motile cocci, catalase-positive and coagulase-negative. Sequence analysis of the 16S rRNA gene, , , and and phylogenetic characteristics revealed that the strains showed the closest relatedness to CCM 4903 and DSM 7068. The strains can be differentiated from by the absence of mannose fermentation and arginine arylamidase and from by the absence of -glucuronidase activity and production of alkaline phosphatase. The chosen type strain ARI 262 shared 20.1 and 31.9 % DNA relatedness with DSM 22147 and CCM 4903, respectively, by DNA–DNA hybridization. iso-C, anteiso-C and iso-C were the most common fatty acids. Cell-wall structure analysis revealed the peptidoglycan type A3 -Lys–Gly-Ser–Gly (type A11.3). The presence of teichoic acid was determined by sequencing the -acetyl---mannosaminyltransferase gene , which is involved in biosynthesis of ribitol teichoic acid. Menaquinone 7 (MK-7) was the predominant respiratory quinone. The G+C content of ARI 262 was 38.8 mol%. The isolated strains represent a novel species of the genus , for which we propose the name sp. nov. The type strain is ARI 262 (=DSM 21968 =CCUG 57266) and strain ARI 602 (=DSM 21969 =CCUG 57267) is a reference strain.

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2010-09-01
2019-10-20
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vol. , part 9, pp. 2042 - 2047

Cluster analysis of partial gene sequences (416 bp) (Fig. S1), gene sequences (960 bp) (Fig. S2), gene sequences (909 bp) (Fig. S3) and gene sequences (554 bp) of strains of sp. nov. compared with strains of species of the genus . [PDF](84 KB)



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