1887

Abstract

A novel, moderately halophilic bacterium belonging to the genus was isolated from crabs preserved in soy sauce: a traditional Korean fermented seafood. Colonies of strain Crm were ivory and the cells were non-motile, Gram-positive cocci. The organism was non-sporulating, catalase-positive and oxidase-negative. The major fatty acids of strain Crm were iso-C (22.0 %), anteiso-C (40.6 %) and anteiso-C (12.1 %). The cell wall peptidoglycan contained lysine and glycine, and the major isoprenoid quinone was MK-6. The polar lipids were phosphatidylglycerol, diphosphatidylglycerol and an unidentified glycolipid. The genomic DNA G+C content was 47.8 mol%. Strain Crm was closely related to the type strain of , with which it shared 96.9 % 16S rRNA gene sequence similarity. The DNA–DNA hybridization value between strains Crm and DSM 19336 was 7.6 %. Based on phenotypic, genetic and phylogenetic data, strain Crm should be classified as a novel species within the genus , for which the name sp. nov. is proposed. The type strain is Crm (=KCTC 13301 =JCM 15796).

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2010-03-01
2019-10-19
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vol. , part 3, pp. 653 - 658

One-dimensional thin-layer chromatogram on a Merck silica gel 60 F254 aluminium-backed plate of the phospholipids (left) and glycolipids (right) from strain Crm and reference strain Salinicoccus halodurans DSM 19336 . Lanes: 1, Salinicoccus halodurans DSM 19336 ; 2, strain Crm The solvent was chloroform/methanol/acetic acid/water (85:22.5:10:4, by vol.). Phospholipids (left) were detected by spraying the plate with Zinzadze reagent and glycolipids (right) were detected by spraying the plate with 0.5% α-naphthol in methanol/water (1:1, v/v) and then with sulfuric acid/ethanol (1:1, v/v), followed by heating at 150 °C for 5 min. PG, Phosphatidylglycerol; DPG, diphosphatidylglycerol; UG, unidentified glycolipid.



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