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Volume 59, Issue 12

sp. nov., a moderately halophilic bacterium from a hypersaline Iranian lake Free

Abstract

A Gram-positive, moderately halophilic bacterium, designated strain HS224, was isolated from the hypersaline lake Howz-Soltan in Iran. Cells of strain HS224 were rod-shaped, motile and produced oval endospores. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain HS224 was affiliated to the genus , exhibiting 98.5 % sequence similarity to the type strain of . Strain HS224 was also related closely to the type strains of (98.0 % 16S rRNA gene sequence similarity), (97.9 %) and (97.0 %). Strain HS224 was able to grow at NaCl concentrations of 1–20 % (w/v), with optimum growth occurring at 10 % (w/v) NaCl. The optimum temperature and pH for growth were 35 °C and pH 7.5. Major polar lipids were phosphatidylglycerol and diphosphatidylglycerol, the isoprenoid quinone was MK-7 and the peptidoglycan type was A1, with -diaminopimelic acid as the diagnostic diamino acid; these characteristics were shared with . The major cellular fatty acids of strain HS224 were anteiso-C, iso-C, anteiso-C and iso-C. The G+C content of the DNA was 37.5 mol%. The level of DNA–DNA relatedness between strain HS224 and JCM 13188 was 30.8 %. It is evident from the genotypic, chemotaxonomic and phenotypic data presented that strain HS224 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is HS224 (=CCM 7596=DSM 21633=JCM 15721=LMG 24786).

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2009-12-01
2024-09-16
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Piscibacillus halophilus sp. nov., a moderately halophilic bacterium from a hypersaline Iranian lake
Int J Syst Evol Microbiol 59, 3095 (2009); https://doi.org/10.1099/ijs.0.012013-0
/content/journal/ijsem/10.1099/ijs.0.012013-0
/content/journal/ijsem/10.1099/ijs.0.012013-0
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Neighbour-joining tree based on 16S rRNA gene sequences, showing the position of strain HS224 among related species. [PDF](19 KB)

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Maximum-likelihood tree based on 16S rRNA gene sequences, showing the position of strain HS224 among related species. [PDF](19 KB)

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Cellular fatty acid profiles of strain HS224 and RBU1-1 . [PDF](17 KB)

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