1887

Abstract

A Gram-positive, moderately halophilic bacterium, designated strain HS224, was isolated from the hypersaline lake Howz-Soltan in Iran. Cells of strain HS224 were rod-shaped, motile and produced oval endospores. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain HS224 was affiliated to the genus , exhibiting 98.5 % sequence similarity to the type strain of . Strain HS224 was also related closely to the type strains of (98.0 % 16S rRNA gene sequence similarity), (97.9 %) and (97.0 %). Strain HS224 was able to grow at NaCl concentrations of 1–20 % (w/v), with optimum growth occurring at 10 % (w/v) NaCl. The optimum temperature and pH for growth were 35 °C and pH 7.5. Major polar lipids were phosphatidylglycerol and diphosphatidylglycerol, the isoprenoid quinone was MK-7 and the peptidoglycan type was A1, with -diaminopimelic acid as the diagnostic diamino acid; these characteristics were shared with . The major cellular fatty acids of strain HS224 were anteiso-C, iso-C, anteiso-C and iso-C. The G+C content of the DNA was 37.5 mol%. The level of DNA–DNA relatedness between strain HS224 and JCM 13188 was 30.8 %. It is evident from the genotypic, chemotaxonomic and phenotypic data presented that strain HS224 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is HS224 (=CCM 7596=DSM 21633=JCM 15721=LMG 24786).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.012013-0
2009-12-01
2021-10-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/12/3095.html?itemId=/content/journal/ijsem/10.1099/ijs.0.012013-0&mimeType=html&fmt=ahah

References

  1. Arahal, D. R., García, M. T., Ludwig, W., Schleifer, K. H. & Ventosa, A.(2001a). Transfer of Halomonas canadensis and Halomonas israelensis to the genus Chromohalobacter, as Chromohalobacter canadensis comb. nov. and Chromohalobacter israelensis comb. nov. Int J Syst Evol Microbiol 51, 1443–1448. [Google Scholar]
  2. Arahal, D. R., García, M. T., Vargas, C., Cánovas, D., Nieto, J. J. & Ventosa, A.(2001b).Chromohalobacter salexigens sp. nov., a moderately halophilic species that includes Halomonas elongata DSM 3043 and ATCC 33174. Int J Syst Evol Microbiol 51, 1457–1462. [Google Scholar]
  3. Baron, E. J. & Finegold, S. M.(1990).Bailey and Scott's Diagnostic Microbiology, 8th edn. St Louis: Mosby.
  4. Chun, J., Lee, J.-H., Jung, Y., Kim, M., Kim, S., Kim, B. K. & Lim, Y.-W.(2007). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57, 2259–2261.[CrossRef] [Google Scholar]
  5. De Ley, J. & Tijtgat, R.(1970). Evaluation of membrane filter methods for DNA-DNA hybridization. Antonie van Leeuwenhoek 36, 461–474.[CrossRef] [Google Scholar]
  6. Felsenstein, J.(1981). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef] [Google Scholar]
  7. Fitch, W. M.(1971). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef] [Google Scholar]
  8. Harrigan, W. F. & McCance, M. E.(1976).Laboratory Methods in Food and Dairy Microbiology. London: Academic Press.
  9. Johnson, J. L.(1994). Similarity analysis of DNAs. In Methods for General and Molecular Bacteriology, pp. 655–681. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  10. Ludwig, W., Strunk, O., Westram, R., Richter, L., Meier, H., Yadhukumar, Buchner, A., Lai, T., Steppi, S. & other authors(2004). ARB: a software environment for sequence data. Nucleic Acids Res 32, 1363–1371.[CrossRef] [Google Scholar]
  11. Marmur, J.(1961). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef] [Google Scholar]
  12. Marmur, J. & Doty, P.(1962). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef] [Google Scholar]
  13. Márquez, M. C., Carrasco, I. J., Xue, Y., Ma, Y., Cowan, D. A., Jones, B. E., Grant, W. D. & Ventosa, A.(2008).Aquisalibacillus elongatus gen. nov., sp. nov., a moderately halophilic bacterium of the family Bacillaceae isolated from a saline lake. Int J Syst Evol Microbiol 58, 1922–1926.[CrossRef] [Google Scholar]
  14. Mata, J. A., Martinez-Canovas, J., Quesada, E. & Bejar, V.(2002). A detailed phenotypic characterization of the type strains of Halomonas species. Syst Appl Microbiol 25, 360–375.[CrossRef] [Google Scholar]
  15. Murray, R. G. E., Doetsch, R. N. & Robinow, C. F.(1994). Determinative and cytological light microscopy. In Methods for General and Molecular Bacteriology, pp. 21–41. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  16. Owen, R. J. & Hill, L. R.(1979). The estimation of base compositions, base pairing and genome size of bacterial deoxyribonucleic acids. In Identification Methods for Microbiologists, 2nd edn, pp. 217–296. Edited by F. A. Skinner & D. W. Lovelock. London: Academic Press.
  17. Quesada, E., Ventosa, A., Ruiz-Berraquero, F. & Ramos-Cormenzana, A.(1984).Deleya halophila, a new species of moderately halophilic bacteria. Int J Syst Bacteriol 34, 287–292.[CrossRef] [Google Scholar]
  18. Ren, P. G. & Zhou, P. J.(2005).Tenuibacillus multivorans gen. nov., sp. nov., a moderately halophilic bacterium isolated from saline soil in Xin-Jiang, China. Int J Syst Evol Microbiol 55, 95–99.[CrossRef] [Google Scholar]
  19. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  20. Schlesner, H., Lawson, P. A., Collins, M. D., Weiss, N., Wehmeyer, U., Völker, H. & Thomm, M.(2001).Filobacillus milensis gen. nov., sp. nov., a new halophilic spore-forming bacterium with Orn-d-Glu-type peptidoglycan. Int J Syst Evol Microbiol 51, 425–431. [Google Scholar]
  21. Smibert, R. M. & Krieg, N. R.(1994). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  22. Stackebrandt, E. & Goebel, B. M.(1994). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef] [Google Scholar]
  23. Stackebrandt, E., Fredericksen, W., Garrity, G. M., Grimont, P. A. D., Kämpfer, P., Maiden, M. C. J., Nesme, X., Rosselló-Mora, R., Swings, J. & other authors(2002). Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 52, 1043–1047.[CrossRef] [Google Scholar]
  24. Tanasupawat, S., Namwong, S., Kudo, T. & Itoh, T.(2007).Piscibacillus salipiscarius gen. nov., sp. nov., a moderately halophilic bacterium from fermented fish (pla-ra) in Thailand. Int J Syst Evol Microbiol 57, 1413–1417.[CrossRef] [Google Scholar]
  25. Ventosa, A., Quesada, E., Rodriguez-Valera, F., Ruiz-Berraquero, F. & Ramos-Cormenzana, A.(1982). Numerical taxonomy of moderately halophilic Gram-negative rods. J Gen Microbiol 128, 1959–1968. [Google Scholar]
  26. Ventosa, A., Gutierrez, M. C., Kamekura, M. & Dyall-Smith, M. L.(1999). Proposal to transfer Halococcus turkmenicus, Halobacterium trapanicum JCM 9743 and strain GSL-11 to Haloterrigena turkmenica gen. nov., comb. nov. Int J Syst Bacteriol 49, 131–136.[CrossRef] [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.012013-0
Loading
/content/journal/ijsem/10.1099/ijs.0.012013-0
Loading

Data & Media loading...

Supplements

Neighbour-joining tree based on 16S rRNA gene sequences, showing the position of strain HS224 among related species. [PDF](19 KB)

PDF

Maximum-likelihood tree based on 16S rRNA gene sequences, showing the position of strain HS224 among related species. [PDF](19 KB)

PDF

Cellular fatty acid profiles of strain HS224 and RBU1-1 . [PDF](17 KB)

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error