1887

Abstract

A bacterial strain designated SBR5 was isolated from the rhizosphere of . A phylogenetic analysis based on the 16S rRNA gene sequence placed the isolate within the genus , being most closely related to RSA19 (98.1 % similarity). The isolate was a Gram-reaction-variable, motile, facultatively anaerobic bacterium, with spores in a terminal position in cells. Starch was utilized and dihydroxyacetone and catalase were produced. Strain SBR5 displayed plant-growth-promoting rhizobacteria characteristics: the ability to fix nitrogen and to produce siderophores and indole-3-acetic acid. The DNA G+C content was 55.1 mol%. Chemotaxonomic analysis of the isolated strain revealed that MK-7 was the predominant menaquinone, while the major fatty acid was anteiso-C. DNA–DNA hybridization values between strain SBR5 and RSA19, TOD45 and KK19 were 43, 35 and 28 %, respectively. These DNA relatedness data and the results of phylogenetic and phenotypic analyses showed that strain SBR5 should be considered as the nitrogen-fixing type strain of a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SBR5 (=CCGB 1313 =CECT 7330).

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2010-01-01
2019-10-21
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