1887

Abstract

A yellow-coloured bacterium, T41, was isolated from a soil sample of a subtropical rainforest in Nepal. Cells were Gram-reaction-positive, aerobic, non-motile, short rods. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain formed a cluster with , , , , , and in the phylum . The strain showed the highest sequence similarity to the type strain of (93.2 %). The major isoprenoid quinone was MK-7 and the predominant cellular fatty acids (>10 %) were iso-15 : 0 (33.8 %), iso-15 : 1 G (13.3 %) and iso-17 : 0 3-OH (12.9 %). The DNA G+C content was 48.1 mol%. On the basis of phenotypic and phylogenetic data and genomic distinctiveness, strain T41 represents a novel species in a new genus in the phylum , for which the name gen. nov., sp. nov. is proposed. The type strain of is strain T41 (=CGMCC 1.7723 =NBRC 106054).

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2010-07-01
2020-09-30
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References

  1. Buck J. D. 1982; Nonstaining (KOH) method for determination of Gram reactions of marine bacteria. Appl Environ Microbiol 44:992–993
    [Google Scholar]
  2. Collins M. D. 1985; Isoprenoid quinone analysis in classification and identification. In Chemical Methods in Bacterial Systematics pp 267–287 Edited by Goodfellow M., Minnikin D. E. London: Academic Press;
    [Google Scholar]
  3. De Ley J. 1970; Reexamination of the association between melting point, buoyant density, and chemical base composition of deoxyribonucleic acid. J Bacteriol 101:737–754
    [Google Scholar]
  4. Di Cello F., Bevivino A., Chiarini L., Fani R., Paffetti D., Tabacchioni S., Dalmastri C. 1997; Biodiversity of a Burkholderia cepacia population isolated from the maize rhizosphere at different plant growth stages. Appl Environ Microbiol 63:4485–4493
    [Google Scholar]
  5. Dong X. Z., Cai M. Y. 2001 General Bacterial Identification System Handbook pp 377–385 Beijing: Scientific Press;
    [Google Scholar]
  6. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  7. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [CrossRef]
    [Google Scholar]
  8. Güssow D., Clackson T. 1989; Direct clone characterization from plaques and colonies by the polymerase chain reaction. Nucleic Acids Res 17: 4000 [CrossRef]
    [Google Scholar]
  9. Kumar S., Tamura K., Nei M. 2004; mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163 [CrossRef]
    [Google Scholar]
  10. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [CrossRef]
    [Google Scholar]
  11. MIDI 1999; Sherlock Microbial Identification System Operating Manual, version 3.0. Newark, DE: MIDI, Inc;
    [Google Scholar]
  12. Rzhetsky A., Nei M. 1992; A simple method for estimating and testing minimum evolution trees. Mol Biol Evol 9:945–967
    [Google Scholar]
  13. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  14. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  15. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  16. Weon H.-Y., Kim B.-Y., Yoo S.-H., Lee S.-Y., Kwon S.-W., Go S.-J., Stackebrandt E. 2006; Niastella koreensis gen. nov., sp. nov. and Niastella yeongjuensis sp. nov.,novel members of the phylum Bacteroidetes , isolated from soil cultivated with Korean ginseng. Int J Syst Evol Microbiol 56:1777–1782 [CrossRef]
    [Google Scholar]
  17. Weon H.-Y., Kim B.-Y., Joa J.-H., Kwon S.-W., Kim W.-G., Koo B.-G. 2008; Niabella soli sp. nov., isolated from soil from Jeju Island, Korea. Int J Syst Evol Microbiol 58:467–469 [CrossRef]
    [Google Scholar]
  18. Xie C. H., Yokota A. 2006; Reclassification of [ Flavobacterium ] ferrugineum as Terrimonas ferruginea gen. nov., comb. nov. and description of Terrimonas lutea sp. nov., isolated from soil. Int J Syst Evol Microbiol 56:1117–1121 [CrossRef]
    [Google Scholar]
  19. Yoon M. H., Im W. T. 2007; Flavisolibacter ginsengiterrae gen. nov., sp. nov. and Flavisolibacter ginsengisoli sp. nov., isolated from ginseng cultivating soil. Int J Syst Evol Microbiol 57:1834–1839 [CrossRef]
    [Google Scholar]
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