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Abstract

A Gram-positive-staining, motile, rod-shaped, endospore-forming bacterium (BFLP-1) was isolated from faeces of wild long-snouted seahorses () captured in north-west Spain (Toralla, Galicia). Strain BFLP-1 grew at 10–30 °C and pH 5.5–9 (optimally at 20 °C and pH 7.2) and with 0–7 % (w/v) NaCl (optimally with 2 % NaCl). The G+C content of the DNA was 48.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain BFLP-1 was a member of the genus and was most closely related to D-1,5a (96.6 %), LMG 18435 (96.0 %) and CVS-8 (95.9 %). Chemotaxonomic data (peptidoglycan type, -diaminopimelic acid; major menaquinone, MK-7; predominant fatty acids, anteiso-C, anteiso-C and C 11; major polar lipids, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unknown aminoglycophospholipid) supported the affiliation of strain BFLP-1 to the genus . Comparative analysis of 16S rRNA gene sequences and chemotaxonomic and phenotypic features indicated that strain BFLP-1 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is BFLP-1 (=DSM 21539 =LMG 24668).

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2010-04-01
2020-01-18
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vol. , part 4, pp. 892 - 895

Maximum-parsimony phylogenetic analysis based on 16S rRNA gene sequences constructed after multiple alignment of data by CLUSTAL X, showing the position of strain BFLP-1 and related members of the genus . [PDF](45 KB)



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