1887

Abstract

A Gram-stain-negative, facultatively anaerobic bacterial strain, designated 717, was isolated from a water sample collected from the Muga river, Girona, north-east Spain. Preliminary analysis of the 16S rRNA gene sequence showed that this strain belonged to the genus , the nearest species being (99.5 % similarity, with seven different nucleotides). A polyphasic study based on a multilocus phylogenetic analysis of five housekeeping genes (, , , and ; 3684 bp) showed isolate 717 to be an independent phylogenetic line, with , and as the closest neighbour species. DNA–DNA reassociation experiments and phenotypic analysis identified that strain 717 represents a novel species, for which the name sp. nov. is proposed, with type strain 717 (=CECT 7401 =LMG 24681).

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2010-01-01
2019-10-20
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vol. , part 1, pp. 72 - 77

Supplementary data [ PDF] (1KB), containing:

Electron microscopy images of strain 717 . (a) Transmission electron microscope, negative stain. Bar, 0.5 µm. (b) Scanning electron microscope. Bar 6 µm.

Unrooted phylogenetic tree derived from 16S rRNA gene sequences showing relationships of strain 717 to all other described species of the genus .

Unrooted phylogenetic tree derived from the MLPA showing the corresponding relationships of strain 717 to all other described species of the genus .

Base differences in the 16S rRNA gene sequence between strain 717 and the type strains of and .

DNA–DNA relatedness between strain 717 and the type strains of , , , and .



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vol. , part 1, pp. 72 - 77

Several corrections to Table 1 (printed on page 74 of the paper) are required. The correct version of Table 1 is shown below.



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