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Abstract

Bacterial strain V2M44 was isolated from forest soil from the Changbai Mountains, Heilongjiang Province, China. Cells of strain V2M44 were Gram-negative, rod-shaped and 0.3–0.4 μm in diameter and 1.5–2.5 μm long. The cells were strictly aerobic and were motile by means of peritrichous flagella. Growth occurred at 20–33 °C (optimum, 29–31 °C), at pH 5–8 (optimum, pH 6.0–7.0) and in the presence of 0−0.1 % (w/v) NaCl (optimum, 0.05 %). Strain V2M44 contained C 6 (38.9 %) and summed feature 7 (comprising C 7, C 9 and/or C 12, 31.5 %) as the major cellular fatty acids and Q-10 as the major respiratory quinone. The major polar lipids were sphingoglycolipid, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol along with two unidentified phospholipids and four unidentified glycolipids. The major component in the polyamine pattern was homospermidine. The DNA G+C content was 65.8 mol%. Analysis of 16S rRNA gene sequences indicated that strain V2M44 was phylogenetically related to members of the genus , with sequence similarities ranging from 92.0–95.8 %. Based on these results, it is concluded that strain V2M44 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is V2M44 (=CGMCC 1.7057=NBRC 104936).

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2010-04-01
2020-01-28
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Polar lipids of strain V2M44 after separation by two-dimensional TLC. [ PDF] 316 KB

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Cellular fatty acid content (%) of strain V2M44 and related type strains of species of the genus [ PDF] 56 KB

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