1887

Abstract

A Gram-negative, non-motile, rod-shaped bacterial strain, JLT354-W, that accumulates poly--hydroxybutyrate granules was isolated from the South China Sea. Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain was related to members of the genera , , and ; levels of similarity between strain JLT354-W and members of the above genera were less than 92.0 %. The predominant fatty acid of strain JLT354-W was C 7 (83.1 %); significant amounts of C (7.9 %) and C 3-OH (3.7 %) were also present. The predominant respiratory ubiquinone was Q-10. The DNA G+C content of strain JLT354-W was 63.7 mol%. The isolate was distinguishable from members of the order based on phenotypic and biochemical characteristics. On the basis of the taxonomic data presented, strain JLT354-W is considered to represent a novel species of a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is JLT354-W (=LMG 24665=CGMCC 1.7290).

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2010-04-01
2024-12-06
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References

  1. Andrews J. M. 2008; BSAC standardized disc susceptibility testing method (Version 7). J Antimicrob Chemother 62:256–278 [CrossRef]
    [Google Scholar]
  2. Barritt M. M. 1936; The intensification of the Voges-Proskauer reaction by the addition of α -naphthol. J Pathol Bacteriol 42:441–445 [CrossRef]
    [Google Scholar]
  3. Biebl H., Allgaier M., Tindall B. J., Koblizek M., Lünsdorf H., Pukall R., Wagner-Döbler I. 2005; Dinoroseobacter shibae gen. nov., sp. nov., a new aerobic phototrophic bacterium isolated from dinoflagellates. Int J Syst Evol Microbiol 55:1089–1096 [CrossRef]
    [Google Scholar]
  4. Buchan A., Neidle E. L., Moran M. A. 2001; Diversity of the ring-cleaving dioxygenase gene pcaH in a salt marsh bacterial community. Appl Environ Microbiol 67:5801–5809 [CrossRef]
    [Google Scholar]
  5. Buchan A., González J. M., Moran M. N. 2005; Overview of the marine Roseobacter lineage. Appl Environ Microbiol 71:5665–5677 [CrossRef]
    [Google Scholar]
  6. Cho J. C., Giovannoni S. J. 2004; Oceanicola granulosus gen. nov., sp. nov. and Oceanicola batsensis sp. nov., poly- β -hydroxybutyrate-producing marine bacteria in the order ‘ Rhodobacterales ’. Int J Syst Evol Microbiol 54:1129–1136 [CrossRef]
    [Google Scholar]
  7. Cho J. C., Stephen J. G. 2006; Pelagibaca bermudensis gen. nov., sp. nov., a novel marine bacterium within the Roseobacter clade in the order Rhodobacterales . Int J Syst Evol Microbiol 56:855–859 [CrossRef]
    [Google Scholar]
  8. Clarke P. H. 1953; Hydrogen sulphide production by bacteria. J Gen Microbiol 8:397–407 [CrossRef]
    [Google Scholar]
  9. Dai X., Wang B. J., Yang Q. X., Jiao N. Z., Liu S. J. 2006; Yangia pacifica gen. nov., sp. nov., a novel member of the Roseobacter clade from coastal sediment of the East China Sea. Int J Syst Evol Microbiol 56:529–533 [CrossRef]
    [Google Scholar]
  10. Dong X. Z., Cai M. Y. 2001 Determinative Manual for Routine Bacteriology Beijing: Scientific Press;
    [Google Scholar]
  11. Embley T. M. 1991; The linear PCR reaction: a simple and robust method for sequencing amplified rRNA genes. Lett Appl Microbiol 13:171–174 [CrossRef]
    [Google Scholar]
  12. Fraser S. L., Jorgensen J. H. 1997; Reappraisal of the antimicrobial susceptibilities of Chryseobacterium and Flavobacterium species and methods for reliable susceptibility testing. Antimicrob Agents Chemother 41:2738–2741
    [Google Scholar]
  13. Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. (editors) 1994 Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology;
    [Google Scholar]
  14. Gonzalez J. M., Mayer F., Moran M. A., Hodson R. E., Whitman W. B. 1997; Sagittula stellata gen. nov., sp. nov., a lignin-transforming bacterium from a coastal environment. Int J Syst Bacteriol 47:773–780 [CrossRef]
    [Google Scholar]
  15. Harwati T. U., Kasai Y., Kodama Y., Susilaningsih D., Watanabe K. 2009; Tropicibacter naphthalenivorans gen. nov., sp. nov., a polycyclic aromatic hydrocarbon-degrading bacterium isolated from Semarang Port in Indonesia. Int J Syst Evol Microbiol 59:392–396 [CrossRef]
    [Google Scholar]
  16. Hiraishi A., Ueda Y., Ishihara J. 1998; Quinone profiling of bacterial communities in natural and synthetic sewage activated sludge for enhanced phosphate removal. Appl Environ Microbiol 64:992–998
    [Google Scholar]
  17. Hwang C. Y., Cho B. C. 2008; Ponticoccus litoralis gen. nov., sp. nov., a marine bacterium in the family Rhodobacteraceae . Int J Syst Evol Microbiol 58:1332–1338 [CrossRef]
    [Google Scholar]
  18. Johnson J. L. 1994; Similarity analysis of DNAs. In Methods for General and Molecular Bacteriology pp 655–681 Edited by Gerhardt P. E., Murray R. G., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  19. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207
    [Google Scholar]
  20. Kumar S., Tamura K., Nei M. 2004; mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Bioinformatics 5:150–163
    [Google Scholar]
  21. Kurahashi M., Yokota A. 2007; Tateyamaria omphalii gen. nov., sp. nov., an α -Proteobacterium isolated from a top shell Omphalius pfeifferi pfeifferi . Syst Appl Microbiol 30:371–375 [CrossRef]
    [Google Scholar]
  22. Labrenz M., Collins M. D., Lawson P. A., Tindall B. J., Braker G., Hirsch P. 1998; Antarctobacter heliothermus gen. nov., sp. nov., a budding bacterium from hypersaline and heliothermal Ekho lake. Int J Syst Bacteriol 48:1363–1372 [CrossRef]
    [Google Scholar]
  23. Macián M. C., Arahal D. R., Garay E., Ludwig W., Schleifer K. H., Pujalte M. J. 2005a; Thalassobacter stenotrophicus gen. nov., sp. nov., a novel marine alpha-proteobacterium isolated from Mediterranean sea water. Int J Syst Evol Microbiol 55:105–110 [CrossRef]
    [Google Scholar]
  24. Macián M. C., Arahal D. R., Garay E., Ludwig W., Schleifer K. H., Pujalte M. J. 2005b; Jannaschia rubra sp. nov., a red-pigmented bacterium isolated from sea water. Int J Syst Evol Microbiol 55:649–653 [CrossRef]
    [Google Scholar]
  25. Mandel M., Igambi L., Bergenda J., Dodson M. L., Scheltge E. 1970; Correlation of melting temperature and cesium chloride buoyant density of bacterial deoxyribonucleic acid. J Bacteriol 101:333–338
    [Google Scholar]
  26. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [CrossRef]
    [Google Scholar]
  27. Mata J. A., Martínez-Cánovas J., Quesada E., Béjar V. 2002; A detailed phenotypic characterisation of the type strains of Halomonas species. Syst Appl Microbiol 25:360–375 [CrossRef]
    [Google Scholar]
  28. Romano I., Lama L., Nicolaus B., Poli A., Gambacorta A., Giordano A. 2006; Halomonas alkaliphila sp. nov., a novel halotolerant alkaliphilic bacterium isolated from a salt pool in Campania (Italy. J Gen Appl Microbiol 52:339–348 [CrossRef]
    [Google Scholar]
  29. Schaefer J. K., Goodwin K. D., McDonald I. R., Murrell J. C., Oremland R. S. 2002; Leisingera methylohalidivorans gen. nov., sp. nov., a marine methylotroph that grows on methyl bromide. Int J Syst Evol Microbiol 52:851–859 [CrossRef]
    [Google Scholar]
  30. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization In Methods for General and Molecular Bacteriology . pp 607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
  31. Vandecandelaere I., Nercessian O., Segaert E., Achouak W., Mollica A., Faimali M., Vandamme P. 2009; Nautella italica gen. nov., sp. nov., isolated from a marine electroactive biofilm. Int J Syst Evol Microbiol 59:811–817 [CrossRef]
    [Google Scholar]
  32. Wagner-Döbler I., Biebl H. 2006; Environmental biology of the marine Roseobacter lineage. Annu Rev Microbiol 60:255–280 [CrossRef]
    [Google Scholar]
  33. Wagner-Döbler I., Rheims H., Felske A., Pukall R., Tindall B. J. 2003; Jannaschia helgolandensis gen. nov., sp. nov., a novel abundant member of the marine Roseobacter clade from the North Sea. Int J Syst Evol Microbiol 53:731–738 [CrossRef]
    [Google Scholar]
  34. Williams S. T., Goodfellow M., Alderson G., Wellington E. M., Sneath P. H., Sackin M. J. 1983; Numerical classification of Streptomyces and related genera. J Gen Microbiol 129:1743–1813
    [Google Scholar]
  35. Ying J. Y., Wang B. J., Dai X., Yang S. S., Liu S. J., Liu Z. P. 2007; Wenxinia marina gen. nov., sp. nov., a novel member of the Roseobacter clade isolated from oilfield sediments of the South China Sea. Int J Syst Evol Microbiol 57:1711–1716 [CrossRef]
    [Google Scholar]
  36. Yurkov V. V., Krieger S., Stackebrandt E., Beatty J. T. 1999; Citromicrobium bathyomarinum , a novel aerobic bacterium isolated from deep-sea hydrothermal vent plume waters that contains photosynthetic pigment-protein complexes. J Bacteriol 181:4517–4525
    [Google Scholar]
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