1887

Abstract

A methanogenic archaeon, strain MK4, was isolated from ancient permafrost after long-term selective anaerobic cultivation. The cells were rods, 2.0–8.0 μm long and 0.40–0.45 μm wide, and stained Gram-negative. Optimal growth was observed at 28 °C and pH 7.0–7.2 and in 0.05 M NaCl. The isolate used H plus CO, methylamine plus H and methanol plus H as sources for growth and methanogenesis. Phylogenetic analysis of the 16S rRNA gene sequence of the strain showed close affinity with (similarity >99 % to the type strain). On the basis of the level of DNA–DNA hybridization (62 %) between strain MK4 and VKM B-1629 and phenotypic and phylogenetic differences, strain MK4 was assigned to a novel species of the genus , sp. nov., with the type strain MK4 (=DSM 19849 =VKM B-2440).

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2010-02-01
2019-12-13
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Phase-contrast micrograph (a) and electron micrograph of an ultrathin section (b) of cells of strain MK4 . Bars, 10 µm (a) and 0.1 µm (b). OM, Outer membrane; CM, cytoplasmic membrane; N, nucleoid.

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Effects of temperature, pH and NaCl concentration on growth of VKM B-1629 and strain MK4 . [PDF](56 KB)

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