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Abstract
A Gram-negative, rod-shaped, deep yellow-pigmented bacterium, designated strain CS5-B1T, was isolated from a soil sample collected from a cliff on Mara Island, south-west of Jeju, Republic of Korea. Comparative 16S rRNA gene sequence analysis showed that strain CS5-B1T fell within the radiation of the genus Chitinophaga with the closest relative being Chitinophaga japonensis IFO 16041T (97.2 % sequence similarity). Levels of 16S rRNA gene sequence similarity between strain CS5-B1T and other species of the genus Chitinophaga were 92.9–95.8 %. The dominant menaquinone was MK-7. The polar lipids contained phosphatidylethanolamine, a ninhydrin-positive lipid and an unknown lipid. The major fatty acids were iso-C15 : 0, C16 : 1 ω5c, C16 : 0 and C18 : 0, with iso-C15 : 0 3-OH and iso-C17 : 0 3-OH as the dominant hydroxy fatty acids. The G+C content of the DNA was 48.7 mol%. DNA-DNA relatedness between strain CS5-B1T and C. japonensis DSM 13484T was 5.8–7.9 %. On the basis of the phenotypic and DNA–DNA hybridization data, this bacterium represents a novel species of the genus Chitinophaga, for which the name Chitinophaga rupis sp. nov. is proposed. The type strain is CS5-B1T (=KCTC 22170T=DSM 21039T).
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