1887

Abstract

A novel Gram-positive bacterium, designated Kis4-28, was isolated from the intestinal tract of a fish, and its taxonomic position was investigated by a polyphasic approach. The sample was collected from the coast of Tokyo Bay, Japan. Cells of strain Kis4-28 were rod-shaped, non-motile and non-sporulating. The peptidoglycan type of the isolate was A4; lysine was the diagnostic diamino acid. The only menaquinone detected was MK-8(H), and the major fatty acids were anteiso-C and C. Galactose was detected as a major cell-wall sugar. The polar lipids were phosphatidylethanolamine and phosphatidylglycerol. The DNA G+C content was 70.7 mol%. 16S rRNA gene sequence analysis revealed that strain Kis4-28 and the type strain of formed a monophyletic cluster with a 16S rRNA gene sequence similarity of 96.2 %. Strain Kis4-28 was clearly distinguishable from the genus in terms of its chemotaxonomic characteristics. On the basis of the genotypic and phenotypic characteristics, a new genus and species is proposed for strain Kis4-28, with the name gen. nov., sp. nov. The type strain of is Kis4-28 (=NBRC 104924 =DSM 21801). In addition, on the basis of 16S rRNA gene sequence analysis of the genus and related genera, emended descriptions of the families and are proposed to accommodate the genera , and , and the genera and , respectively.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.011106-0
2009-11-01
2020-11-30
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/11/2809.html?itemId=/content/journal/ijsem/10.1099/ijs.0.011106-0&mimeType=html&fmt=ahah

References

  1. Altenburger, P., Kämpfer, P., Schumann, P., Vybiral, D., Lubitz, W. & Busse, H.-J. ( 2002; ). Georgenia muralis gen. nov., sp. nov., a novel actinobacterium isolated from a medieval wall painting. Int J Syst Evol Microbiol 52, 875–881.[CrossRef]
    [Google Scholar]
  2. Ewing, W. H., Davis, B. R. & Reavis, R. W. ( 1957; ). Phenylalanine and malonate media and their use in enteric bacteriology. Public Health Lab 15, 153–161.
    [Google Scholar]
  3. Felsenstein, J. ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef]
    [Google Scholar]
  4. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 738–791.
    [Google Scholar]
  5. Gerhardt, P., Murray, R. G. E., Wood, W. A. & Krieg, N. R. ( 1994; ). Methods for General and Molecular Bacteriology. Washington, DC: American Society for Microbiology.
  6. Groth, I., Schumann, P., Rainey, F. A., Martin, K., Schuetze, B. & Augsten, K. ( 1997; ). Bogoriella caseilytica gen. nov., sp. nov., a new alkaliphilic actinomycete from a soda lake in Africa. Int J Syst Bacteriol 47, 788–794.[CrossRef]
    [Google Scholar]
  7. Groth, I., Schumann, P., Schuetze, B., Augsten, K., Kramer, I. & Stackebrandt, E. ( 1999; ). Beutenbergia cavernae gen. nov., sp. nov., an l-lysine-containing actinomycete isolated from a cave. Int J Syst Bacteriol 49, 1733–1740.[CrossRef]
    [Google Scholar]
  8. Gu, Q., Paściak, M., Luo, H., Gamian, A., Liu, Z. & Huang, Y. ( 2007; ). Ruania albidiflava gen. nov., sp. nov., a novel member of the suborder Micrococcineae. Int J Syst Evol Microbiol 57, 809–814.[CrossRef]
    [Google Scholar]
  9. Iino, T., Mori, K., Tanaka, K., Suzuki, K. & Harayama, S. ( 2007; ). Oscillibacter valericigenes gen. nov., sp. nov., a valerate-producing anaerobic bacterium isolated from the alimentary canal of a Japanese corbicula clam. Int J Syst Evol Microbiol 57, 1840–1845.[CrossRef]
    [Google Scholar]
  10. Li, W.-J., Schumann, P., Zhang, Y.-Q., Xu, P., Chen, G.-Z., Xu, L.-H., Stackebrandt, E. & Jiang, C.-L. ( 2005; ). Proposal of Yaniaceae fam. nov. and Yania flava sp. nov. and emended description of the genus Yania. Int J Syst Evol Microbiol 55, 1933–1938.[CrossRef]
    [Google Scholar]
  11. Li, W.-J., Zhi, X.-Y. & Euzéby, J. P. ( 2008; ). Proposal of Yaniellaceae fam. nov., Yaniella gen. nov. and Sinobaca gen. nov. as replacements for the illegitimate prokaryotic names Yaniaceae Li et al. 2005, Yania Li et al. 2004, emend Li et al. 2005, and Sinococcus Li et al. 2006, respectively. Int J Syst Evol Microbiol 58, 525–527.[CrossRef]
    [Google Scholar]
  12. Minnikin, D. E., Alshamaony, L. & Goodfellow, M. ( 1975; ). Differentiation of Mycobacterium, Nocardia and related taxa by thin-layer chromatographic analysis of whole-organism methanolysates. J Gen Microbiol 88, 200–204.[CrossRef]
    [Google Scholar]
  13. Nozawa, Y., Sakai, N., Arai, K., Kawasaki, Y. & Harada, K. ( 2007; ). Reliable and sensitive analysis of amino acids in the peptidoglycan of actinomycetes using the advanced Marfey's method. J Microbiol Methods 70, 306–311.[CrossRef]
    [Google Scholar]
  14. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  15. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  16. Schleifer, K. H. & Kandler, O. ( 1972; ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36, 407–477.
    [Google Scholar]
  17. Stackebrandt, E. & Schumann, P. ( 2000; ). Description of Bogoriellaceae fam. nov., Dermacoccaceae fam. nov., Rarobacteraceae fam. nov. and Sanguibacteraceae fam. nov. and emendation of some families of the suborder Micrococcineae. Int J Syst Evol Microbiol 50, 1279–1285.[CrossRef]
    [Google Scholar]
  18. Stackebrandt, E., Rainey, F. A. & Ward-Rainey, N. L. ( 1997; ). Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47, 479–491.[CrossRef]
    [Google Scholar]
  19. Tamura, T. & Hatano, K. ( 2001; ). Phylogenetic analysis of the genus Actinoplanes and transfer of Actinoplanes minutisporangius Ruan et al. 1986 and ‘Actinoplanes aurantiacus’ to Cryptosporangium minutisporangium comb. nov. and Cryptosporangium aurantiacum sp. nov. Int J Syst Evol Microbiol 51, 2119–2125.[CrossRef]
    [Google Scholar]
  20. Tamura, T., Nakagaito, Y., Nishi, T., Hasegawa, T., Stackebrandt, E. & Yokota, A. ( 1994; ). A new genus of the order Actinomycetales, Couchioplanes gen. nov., with descriptions of Couchioplanes caeruleus (Horan and Brodsky 1986) comb. nov. and Couchioplanes caeruleus subsp. azureus subsp. nov. Int J Syst Bacteriol 44, 193–203.[CrossRef]
    [Google Scholar]
  21. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  22. Uchida, K., Kudo, T., Suzuki, K. & Nakase, T. ( 1999; ). A new rapid method of glycolate test by diethyl ether extraction, which is applicable to a small amount of bacterial cells of less than one milligram. J Gen Appl Microbiol 45, 49–56.[CrossRef]
    [Google Scholar]
  23. von Wintzingerode, F., Göbel, U. B., Siddiqui, R. A., Rösick, U., Schumann, P., Frühling, A., Rohde, M., Pukall, R. & Stackebrandt, E. ( 2001; ). Salana multivorans gen. nov., sp. nov., a novel actinobacterium isolated from an anaerobic bioreactor and capable of selenate reduction. Int J Syst Evol Microbiol 51, 1653–1661.[CrossRef]
    [Google Scholar]
  24. Zhi, X.-Y., Li, W.-J. & Stackebrandt, E. ( 2009; ). An update of the structure and 16S rRNA gene sequence-based definition of higher ranks of the class Actinobacteria, with the proposal of two new suborders and four new families and emended descriptions of the existing higher taxa. Int J Syst Evol Microbiol 59, 589–608.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.011106-0
Loading
/content/journal/ijsem/10.1099/ijs.0.011106-0
Loading

Data & Media loading...

Supplements

vol. , part 11, pp. 2809 - 2814

Phylogenetic tree derived from 16S rRNA gene sequences of strain Kis4-28T and some members of the suborder . [ PDF] 26kb



PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error