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Abstract

A moderately halophilic, endospore-forming, Gram-positive, catalase- and oxidase-positive, motile, rod-shaped, aerobic bacterium, designated strain JSM 078133, was isolated from a subterranean brine sample collected from a salt mine in Hunan Province, China. Strain JSM 078133 was able to grow with 1–23 % (w/v) total salts (optimum, 5–10 %) and at pH 6.0–10.0 (optimum, pH 7.5) and 10–45 °C (optimum, 30 °C). -Diaminopimelic acid was present in the cell-wall peptidoglycan. The predominant respiratory quinone was menaquinone 7 (MK-7) and the major cellular fatty acids were anteiso-C, anteiso-C and iso-C. The genomic DNA G+C content of strain JSM 078133 was 42.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain JSM 078133 belongs to the genus , and was related most closely to the type strains of (97.1 % sequence similarity), (97.0 %) and (96.8 %). The combination of phylogenetic analysis, DNA–DNA relatedness data, phenotypic characteristics and chemotaxonomic data supported the view that strain JSM 078133 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is JSM 078133 (=DSM 21185=KCTC 13236).

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2009-10-01
2019-10-15
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References

  1. Amoozegar, M. A., Malekzadeh, F., Malik, K. A., Schumann, P. & Spröer, C. ( 2003; ). Halobacillus karajensis sp. nov., a novel moderate halophile. Int J Syst Evol Microbiol 53, 1059–1063.[CrossRef]
    [Google Scholar]
  2. An, S.-Y., Kanoh, K., Kasai, H., Goto, K. & Yokota, Y. ( 2007; ). Halobacillus faecis sp. nov., a spore-forming bacterium isolated from a mangrove area on Ishigaki Island, Japan. Int J Syst Evol Microbiol 57, 2476–2479.[CrossRef]
    [Google Scholar]
  3. Atlas, R. M. ( 1993; ). Handbook of Microbiological Media. Edited by L. C. Parks. Boca Raton, FL: CRC Press.
  4. Chen, Y.-G., Cui, X.-L., Pukall, R., Li, H.-M., Yang, Y.-L., Xu, L.-H., Wen, M.-L., Peng, Q. & Jiang, C.-L. ( 2007; ). Salinicoccus kunmingensis sp. nov., a moderately halophilic bacterium isolated from a salt mine in Yunnan, south-west China. Int J Syst Evol Microbiol 57, 2327–2332.[CrossRef]
    [Google Scholar]
  5. Claus, D., Fahmy, F., Rolf, H. J. & Tosunoglu, N. ( 1983; ). Sporosarcina halophila sp. nov., an obligate, slightly halophilic bacterium from salt marsh soils. Syst Appl Microbiol 4, 496–506.[CrossRef]
    [Google Scholar]
  6. Cowan, S. T. & Steel, K. J. ( 1965; ). Manual for the Identification of Medical Bacteria. London: Cambridge University Press.
  7. Cui, X.-L., Mao, P.-H., Zeng, M., Li, W.-J., Zhang, L.-P., Xu, L.-H. & Jiang, C.-L. ( 2001; ). Streptomonospora salina gen. nov., sp. nov., a new member of the family Nocardiopsaceae. Int J Syst Evol Microbiol 51, 357–363.
    [Google Scholar]
  8. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  9. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  10. Felsenstein, J. ( 1997; ). An alternative least-squares approach to inferring phylogenies from pairwise distances. Syst Biol 46, 101–111.[CrossRef]
    [Google Scholar]
  11. Gregersen, T. ( 1978; ). Rapid method for distinction of Gram-negative from Gram-positive bacteria. Eur J Appl Microbiol Biotechnol 5, 123–127.[CrossRef]
    [Google Scholar]
  12. Groth, I., Schumann, P., Weiss, N., Martin, K. & Rainey, F. A. ( 1996; ). Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46, 234–239.[CrossRef]
    [Google Scholar]
  13. Hasegawa, T., Takizawa, M. & Tanida, S. ( 1983; ). A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29, 319–322.[CrossRef]
    [Google Scholar]
  14. Hopwood, D. A., Bibb, M. J., Chater, K. F., Kieser, T., Bruton, C. J., Kieser, H. M., Lydiate, D. J., Smith, C. P. & Ward, J. M. ( 1985; ). Preparation of chromosomal, plasmid and phage DNA. In Genetic Manipulation of Streptomyces: a Laboratory Manual, pp. 79–80. Norwich, UK: John Innes Foundation.
  15. Hua, N.-P., Kanekiyo, A., Fujikura, K., Yasuda, H. & Naganuma, T. ( 2007; ). Halobacillus profundi sp. nov. and Halobacillus kuroshimensis sp. nov., moderately halophilic bacteria isolated from a deep-sea methane cold seep. Int J Syst Evol Microbiol 57, 1243–1249.[CrossRef]
    [Google Scholar]
  16. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  17. Kluge, A. G. & Farris, F. S. ( 1969; ). Quantitative phyletics and the evolution of anurans. Syst Zool 18, 1–32.[CrossRef]
    [Google Scholar]
  18. Liu, W. Y., Zeng, J., Wang, L., Dou, Y. T. & Yang, S. S. ( 2005; ). Halobacillus dabanensis sp. nov. and Halobacillus aidingensis sp. nov., isolated from salt lakes in Xinjiang, China. Int J Syst Evol Microbiol 55, 1991–1996.[CrossRef]
    [Google Scholar]
  19. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  20. Romano, I., Finore, L., Nicolaus, G., Huertas, F. J., Lama, L., Nicolaus, B. & Poli, A. ( 2008; ). Halobacillus alkaliphilus sp. nov., a halophilic bacterium isolated from a salt lake in Fuente de Piedra, southern Spain. Int J Syst Evol Microbiol 58, 886–890.[CrossRef]
    [Google Scholar]
  21. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  22. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  23. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  24. Soto-Ramírez, N., Sánchez-Porro, C., Rosas-Padilla, S., Almodóvar, K., Jiménez, G., Machado-Rodríguez, M., Zapata, M., Ventosa, A. & Montalvo-Rodríguez, R. ( 2008; ). Halobacillus mangrovi sp. nov., a moderately halophilic bacterium isolated from the black mangrove Avicennia germinans. Int J Syst Evol Microbiol 58, 125–130.[CrossRef]
    [Google Scholar]
  25. Spring, S., Ludwig, W., Marquez, M. C., Ventosa, A. & Schleifer, K.-H. ( 1996; ). Halobacillus gen. nov., with descriptions of Halobacillus litoralis sp. nov. and Halobacillus trueperi sp. nov., and transfer of Sporosarcina halophila to Halobacillus halophilus comb. nov. Int J Syst Bacteriol 46, 492–496.[CrossRef]
    [Google Scholar]
  26. Tamura, K., Dudley, J., Nei, M. & Kumar, S. ( 2007; ). mega4: molecular evolutionary genetic analysis (mega) software version 4.0. Mol Biol Evol 24, 1596–1599.[CrossRef]
    [Google Scholar]
  27. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  28. Ventosa, A., Quesada, E., Rodriguez-Valera, F., Ruiz-Berraquero, F. & Ramos-Cormenzana, A. ( 1982; ). Numerical taxonomy of moderately halophilic Gram-negative rods. J Gen Microbiol 128, 1959–1968.
    [Google Scholar]
  29. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  30. Yoon, J.-H., Kang, K. H. & Park, Y.-H. ( 2003; ). Halobacillus salinus sp. nov., isolated from a salt lake on the coast of the East Sea in Korea. Int J Syst Evol Microbiol 53, 687–693.[CrossRef]
    [Google Scholar]
  31. Yoon, J.-H., Kang, K. H., Oh, T.-K. & Park, Y.-H. ( 2004; ). Halobacillus locisalis sp. nov., a halophilic bacterium isolated from a marine solar saltern of the Yellow Sea in Korea. Extremophiles 8, 23–28.[CrossRef]
    [Google Scholar]
  32. Yoon, J.-H., Kang, S.-J., Lee, C.-H., Oh, H. W. & Oh, T.-K. ( 2005; ). Halobacillus yeomjeoni sp. nov., isolated from a marine solar saltern in Korea. Int J Syst Evol Microbiol 55, 2413–2417.[CrossRef]
    [Google Scholar]
  33. Yoon, J.-H., Kang, S.-J., Jung, Y.-T. & Oh, T.-K. ( 2007; ). Halobacillus campisalis sp. nov., containing meso-diaminopimelic acid in the cell-wall peptidoglycan, and emended description of the genus Halobacillus. Int J Syst Evol Microbiol 57, 2021–2025.[CrossRef]
    [Google Scholar]
  34. Yoon, J.-H., Kang, S.-J. & Oh, T.-K. ( 2008; ). Halobacillus seohaensis sp. nov., isolated from a marine solar saltern in Korea. Int J Syst Evol Microbiol 58, 622–627.[CrossRef]
    [Google Scholar]
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