1887

Abstract

A novel actinomycete strain, designated YIM 61105, was isolated from a leaf of from the tropical rainforest in Xishuangbanna, Yunnan Province, south-west China. A 16S rRNA gene sequence analysis revealed that the organism belonged to the phylogenetic cluster of the genus and was most closely related to HMC10 (98.2 %), ‘’ S136 (97.9 %), GW 14-1925 (97.5 %) and DSM 43926 (97.4 %). The 16S rRNA gene sequence similarities to other species were less than 97.4 %. The main chemotaxonomic properties of strain YIM 61105, such as the principal amino acid of the peptidoglycan, the predominant menaquinone and the polar lipid profile, supported its classification within the genus . Strain YIM 61105 was also readily differentiated from closely related species on the basis of a broad range of phenotypic properties and DNA–DNA hybridization values. Thus, this isolate is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is YIM 61105 (=DSM 45220=CCTCC AA 208016).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.010660-0
2009-11-01
2019-10-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/11/2747.html?itemId=/content/journal/ijsem/10.1099/ijs.0.010660-0&mimeType=html&fmt=ahah

References

  1. Collins, M. D. & Jones, D. ( 1980; ). Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acid. J Appl Bacteriol 48, 459–470.[CrossRef]
    [Google Scholar]
  2. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  3. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  4. Fischer, A., Kroppenstedt, R. M. & Stackebrandt, E. ( 1983; ). Molecular-genetic and chemotaxonomic studies on Actinomadura and Nocardiopsis. J Gen Microbiol 129, 3433–3446.
    [Google Scholar]
  5. Gordon, R. E., Barnett, D. A., Handerhan, J. E. & Pang, C. H.-N. ( 1974; ). Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 24, 54–63.[CrossRef]
    [Google Scholar]
  6. Groth, I., Schumann, P., Rainey, F. A., Martin, K., Schuetze, B. & Augsten, K. ( 1997; ). Demetria terragena gen. nov., sp. nov., a new genus of actinomycetes isolated from compost soil. Int J Syst Bacteriol 47, 1129–1133.[CrossRef]
    [Google Scholar]
  7. Hasegawa, T., Takizawa, M. & Tanida, S. ( 1983; ). A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29, 319–322.[CrossRef]
    [Google Scholar]
  8. Hayakawa, M. & Nonomura, H. ( 1987; ). Humic acid-vitamin agar, a new medium for selective isolation of soil actinomycetes. J Ferment Technol 65, 501–509.[CrossRef]
    [Google Scholar]
  9. He, L., Li, W., Huang, Y., Wang, L., Liu, Z., Lanoot, B., Vancanneyt, M. & Swings, J. ( 2005; ). Streptomyces jietaisiensis sp. nov., isolated from soil in northern China. Int J Syst Evol Microbiol 55, 1939–1944.[CrossRef]
    [Google Scholar]
  10. Hozzein, W. N. & Goodfellow, M. ( 2007; ). Nonomuraea aegyptia sp. nov., a novel actinomycete isolated from a sand dune. Antonie Van Leeuwenhoek 92, 165–171.[CrossRef]
    [Google Scholar]
  11. Hugo, W. B. & Russell, A. D. ( 1983; ). Pharmaceutical Microbiology, 3rd edn. Blackwell: Oxford.
  12. Kämpfer, P. & Kroppenstedt, R. M. ( 1996; ). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42, 989–1005.[CrossRef]
    [Google Scholar]
  13. Kämpfer, P., Kroppenstedt, R. M. & Grün-Wollny, I. ( 2005; ). Nonomuraea kuesteri sp. nov. Int J Syst Evol Microbiol 55, 847–851.[CrossRef]
    [Google Scholar]
  14. Kelly, K. L. ( 1964; ). Inter-Society Color Council – National Bureau of Standards Color Name Charts Illustrated with Centroid Colors. Washington, DC: US Government Printing Office.
  15. Kluge, A. G. & Farris, J. S. ( 1969; ). Quantitative phyletics and the evolution of anurans. Syst Zool 18, 1–32.[CrossRef]
    [Google Scholar]
  16. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  17. Lechevalier, M. P. & Lechevalier, H. A. ( 1970; ). Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20, 435–443.[CrossRef]
    [Google Scholar]
  18. Lechevalier, M. P., De Bièvre, C. & Lechevalier, H. A. ( 1977; ). Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochem Syst Ecol 5, 249–260.[CrossRef]
    [Google Scholar]
  19. Li, W.-J., Xu, P., Schumann, P., Zhang, Y.-Q., Pukall, R., Xu, L.-H., Stackebrandt, E. & Jiang, C.-L. ( 2007; ). Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China) and emended description of the genus Georgenia. Int J Syst Evol Microbiol 57, 1424–1428.[CrossRef]
    [Google Scholar]
  20. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  21. Minnikin, D. E., Collins, M. D. & Goodfellow, M. ( 1979; ). Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Bacteriol 47, 87–95.[CrossRef]
    [Google Scholar]
  22. Nonomura, H. & Ohara, Y. ( 1971; ). Distribution of actinomycetes in soil. XI. Some new species of the genus Actinomadura Lechevalier et al. J Ferment Technol 49, 904–912.
    [Google Scholar]
  23. Poscher, J., Kroppenstedt, R. M., Fischer, A. & Stackebrandt, E. ( 1985; ). DNA:DNA reassociation and chemotaxonomic studies on Actinomadura, Microbispora, Microtetraspora, Micropolyspora and Nocardiopsis. Syst Appl Microbiol 6, 264–270.[CrossRef]
    [Google Scholar]
  24. Qin, S., Wang, H.-B., Chen, H.-H., Zhang, Y.-Q., Jiang, C.-L., Xu, L.-H. & Li, W.-J. ( 2008; ). Glycomyces endophyticus sp. nov., an endophytic actinomycete isolated from the root of Carex baccans Nees. Int J Syst Evol Microbiol 58, 2525–2528.[CrossRef]
    [Google Scholar]
  25. Quintana, E., Maldonado, L. & Goodfellow, M. ( 2003; ). Nonomuraea terrinata sp. nov., a novel soil actinomycete. Antonie Van Leeuwenhoek 84, 1–6.[CrossRef]
    [Google Scholar]
  26. Roes, M. L. & Meyers, P. R. ( 2008; ). Nonomuraea candida sp. nov., a new species from South African soil. Antonie Van Leeuwenhoek 93, 133–139.[CrossRef]
    [Google Scholar]
  27. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  28. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  29. Shirling, E. B. & Gottlieb, D. ( 1966; ). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef]
    [Google Scholar]
  30. Stackebrandt, E. & Ebers, J. ( 2006; ). Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 33, 152–155.
    [Google Scholar]
  31. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  32. Stackebrandt, E., Wink, J., Steiner, U. & Kroppenstedt, R. M. ( 2001; ). Nonomuraea dietzii sp. nov. Int J Syst Evol Microbiol 51, 1437–1441.
    [Google Scholar]
  33. Tamura, T., Suzuki, S. & Hatano, K. ( 2000; ). Acrocarpospora gen. nov., a new genus of the order Actinomycetales. Int J Syst Evol Microbiol 50, 1163–1171.[CrossRef]
    [Google Scholar]
  34. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  35. Waksman, S. A. ( 1967; ). The Actinomycetes. A Summary of Current Knowledge. New York: Ronald Press.
  36. Zhang, Z. S., Wang, Y. & Ruan, J. S. ( 1998; ). Reclassification of Thermomonospora and Microtetraspora. Int J Syst Bacteriol 48, 411–422.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.010660-0
Loading
/content/journal/ijsem/10.1099/ijs.0.010660-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error