1887

Abstract

A Gram-positive, moderately halophilic bacterium, designated strain HS286, was isolated from water of the hypersaline Lake Howz-Soltan in Iran. Cells were strictly aerobic, rod-shaped, motile and able to produce ellipsoidal endospores at a central-subterminal position in swollen sporangia. Isolate HS286 grew in a complex medium supplemented with 1–15 % (w/v) NaCl, with optimum growth at 8.0 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequence comparisons showed that strain HS286 was closely related to s G-19.1 (99.4 % gene sequence similarity). The other closest species were MSS-402 (96.9 %) and other species of the genus (with 96.7–93.5 % similarity). Strain HS286 had cell-wall peptidoglycan based on -diaminopimelic acid and MK-7 as the respiratory isoprenoid quinone. The major fatty acids were anteiso-C (43.8 %), iso-C (21.4 %), iso-C (9.4 %), anteiso-C (8.7 %) and iso-C (7.0 %) and the polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylglycerol, two phospholipids and a glycolipid. The DNA G+C content was 43.0 mol%. All of these features confirmed the placement of isolate HS286 within the genus . However DNA–DNA hybridization between strain HS286 and the only recognized species of the genus , s G-19.1, was 27.3 %, showing unequivocally that the novel isolate constituted a new genospecies. Strain HS286 could be clearly differentiated from and other phylogenetic neighbours on the basis of several phenotypic, genotypic and chemotaxonomic features. Therefore, strain HS286 constitutes a novel species, for which the name sp. nov. is proposed. The type strain is HS286 (=CCM 7597=JCM 15722).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.010488-0
2009-10-01
2019-10-23
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/10/2565.html?itemId=/content/journal/ijsem/10.1099/ijs.0.010488-0&mimeType=html&fmt=ahah

References

  1. Amoozegar, M. A., Malekzadeh, F., Malik, K. A., Schumann, P. & Spröer, C. ( 2003; ). Halobacillus karajensis sp. nov., a novel moderate halophile. Int J Syst Evol Microbiol 53, 1059–1063.[CrossRef]
    [Google Scholar]
  2. An, S.-Y., Kanoh, K., Kasai, H., Goto, K. & Yokota, A. ( 2007; ). Halobacillus faecis sp. nov., a spore-forming bacterium isolated from a mangrove area on Ishigaki Island, Japan. Int J Syst Evol Microbiol 57, 2476–2479.[CrossRef]
    [Google Scholar]
  3. Arahal, D. R. & Ventosa, A. ( 2002; ). Moderately halophilic and halotolerant species of Bacillus and related genera. In Applications and Systematics of Bacillus and Relatives, pp. 83–99. Edited by R. Berkeley, M. Heyndrickx, N. Logan & P. De Vos. Oxford: Blackwell.
  4. Baron, E. J. & Finegold, S. M. ( 1990; ). Bailey and Scott's Diagnostic Microbiology, 8th edn. St Louis: Mosby.
  5. Chun, J., Lee, J.-H., Jung, Y., Kim, M., Kim, S., Kim, B. K. & Lim, Y.-W. ( 2007; ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57, 2259–2261.[CrossRef]
    [Google Scholar]
  6. De Ley, J. & Tijtgat, R. ( 1970; ). Evaluation of membrane filter methods for DNA-DNA hybridization. Antonie van Leeuwenhoek 36, 461–474.[CrossRef]
    [Google Scholar]
  7. Felsenstein, J. ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef]
    [Google Scholar]
  8. Fitch, W. M. ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef]
    [Google Scholar]
  9. García, M. T., Gallego, V., Ventosa, A. & Mellado, E. ( 2005; ). Thalassobacillus devorans gen. nov., sp. nov., a moderately halophilic, phenol-degrading, Gram-positive bacterium. Int J Syst Evol Microbiol 55, 1789–1795.[CrossRef]
    [Google Scholar]
  10. Harrigan, W. F. & McCance, M. E. ( 1976; ). Laboratory Methods in Food and Dairy Microbiology. London: Academic Press.
  11. Hua, N.-P., Kanekiyo, A., Fujikura, K., Yasuda, H. & Naganuma, T. ( 2007; ). Halobacillus profundi sp. nov., and Halobacillus kuroshimensis sp. nov., moderately halophilic bacteria isolated from a deep-sea methane cold seep. Int J Syst Evol Microbiol 57, 1243–1249.[CrossRef]
    [Google Scholar]
  12. Johnson, J. L. ( 1994; ). Similarity analysis of DNAs. In Methods for General and Molecular Bacteriology, pp. 655–681. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  13. Liu, W. Y., Zeng, J., Wang, L., Dou, Y. T. & Yang, S. S. ( 2005; ). Halobacillus dabanensis sp. nov. and Halobacillus aidingensis sp. nov., isolated from salt lakes in Xinjiang, China. Int J Syst Evol Microbiol 55, 1991–1996.[CrossRef]
    [Google Scholar]
  14. Ludwig, W., Strunk, O., Westram, R., Richter, L., Meier, H., Yadhukumar, Buchner, A., Lai, T., Steppi, S. & other authors ( 2004; ). arb: a software environment for sequence data. Nucleic Acids Res 32, 1363–1371.[CrossRef]
    [Google Scholar]
  15. Maidak, B. L., Olsen, G. J., Larsen, N., Overbeek, R., McCaughey, M. J. & Woese, C. R. ( 1996; ). The Ribosomal Database Project (RDP). Nucleic Acids Res 24, 82–85.[CrossRef]
    [Google Scholar]
  16. Marmur, J. ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef]
    [Google Scholar]
  17. Marmur, J. & Doty, P. ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef]
    [Google Scholar]
  18. Mata, J. A., Martinez-Canovas, J., Quesada, E. & Bejar, V. ( 2002; ). A detailed phenotypic characterization of the type strains of Halomonas species. Syst Appl Microbiol 25, 360–375.[CrossRef]
    [Google Scholar]
  19. Mellado, E., Moore, E. R. B., Nieto, J. J. & Ventosa, A. ( 1995; ). Phylogenetic inferences and taxonomic consequences of 16S ribosomal DNA sequence comparison of Chromohalobacter marismortui, Volcaniella eurihalina and Deleya salina, and reclassification of V. eurihalina as Halomonas eurihalina comb. nov. Int J Syst Bacteriol 45, 712–716.[CrossRef]
    [Google Scholar]
  20. Murray, R. G. E., Doetsch, R. N. & Robinow, F. ( 1994; ). Determinative and cytological light microscopy. In Methods for General and Molecular Bacteriology, pp. 21–41. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  21. Owen, R. J. & Hill, L. R. ( 1979; ). The estimation of base compositions, base pairing and genome sizes of bacterial deoxyribonucleic acids. In Identification Methods for Microbiologists (Society for Applied Bacteriology Technical Series no. 14), 2nd edn, pp. 277–296. Edited by F. A. Skinner & D. W. Lovelock. London: Academic Press.
  22. Quesada, E., Ventosa, A., Ruiz-Berraquero, F. & Ramos-Cormenzana, A. ( 1984; ). Deleya halophila, a new species of moderately halophilic bacteria. Int J Syst Bacteriol 34, 287–292.[CrossRef]
    [Google Scholar]
  23. Romano, I., Finore, I., Nicolaus, G., Huertas, F. J., Lama, L., Nicolaus, B. & Poli, A. ( 2008; ). Halobacillus alkaliphilus sp. nov., a halophilic bacterium isolated from a salt lake in Fuente de Piedra, southern Spain. Int J Syst Evol Microbiol 58, 886–890.[CrossRef]
    [Google Scholar]
  24. Saitou, N. & Nei, M. ( 1987; ). The neighbour-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  25. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  26. Spring, S., Ludwig, W., Marquez, M. C., Ventosa, A. & Schleifer, K. H. ( 1996; ). Halobacillus gen. nov., with descriptions of Halobacillus litoralis sp. nov. and Halobacillus trueperi sp. nov., and transfer of Sporosarcina halophila to Halobacillus halophilus comb. nov. Int J Syst Bacteriol 46, 492–496.[CrossRef]
    [Google Scholar]
  27. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  28. Stackebrandt, E., Fredericksen, W., Garrity, G. M., Grimont, P. A. D., Kämpfer, P., Maiden, M. C. J., Nesme, X., Rossello-Mora, R., Swings, J. & other authors ( 2002; ). Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 52, 1043–1047.[CrossRef]
    [Google Scholar]
  29. Ventosa, A. ( 2006; ). Unusual micro-organisms from unusual habitats: hypersaline environments. In Prokaryotic diversity: Mechanisms and Significance, pp. 223–253. Edited by N. A. Logan, H. M. Lappin-Scott & P. C. F. Oyston. Cambridge: Cambridge University Press.
  30. Ventosa, A., Quesada, E., Rodriguez-Valera, F., Ruiz-Berraquero, F. & Ramos-Cormenzana, A. ( 1982; ). Numerical taxonomy of moderately halophilic Gram-negative rods. J Gen Microbiol 128, 1959–1968.
    [Google Scholar]
  31. Ventosa, A., Nieto, J. J. & Oren, A. ( 1998; ). Biology of moderately halophilic aerobic bacteria. Microbiol Mol Biol Rev 62, 504–544.
    [Google Scholar]
  32. Yoon, J.-H., Kang, K. H. & Park, Y.-H. ( 2003; ). Halobacillus salinus sp. nov., isolated from a salt lake on the coast of the East Sea in Korea. Int J Syst Evol Microbiol 53, 687–693.[CrossRef]
    [Google Scholar]
  33. Yoon, J.-H., Kang, K. H., Oh, T.-K. & Park, Y.-H. ( 2004; ). Halobacillus locisalis sp. nov., a halophilic bacterium isolated from a marine solar saltern of the Yellow Sea in Korea. Extremophiles 8, 23–28.[CrossRef]
    [Google Scholar]
  34. Yoon, J.-H., Kang, K. H., Lee, C.-H., Oh, H.-W. & Oh, T.-K. ( 2005; ). Halobacillus yeomjeoni sp. nov., isolated from a marine solar saltern in Korea. Int J Syst Evol Microbiol 55, 2413–2417.[CrossRef]
    [Google Scholar]
  35. Yoon, J.-H., Kang, J. K., Jung, Y.-T. & Oh, T.-K. ( 2007; ). Halobacillus campisalis sp. nov., containing meso-diaminopimelic acid in the cell-wall peptidoglycan, and emended description of the genus Halobacillus. Int J Syst Evol Microbiol 57, 2021–2025.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.010488-0
Loading
/content/journal/ijsem/10.1099/ijs.0.010488-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error