A Gram-positive, moderately halophilic, alkalitolerant, filamentous, aerobic actinomycete, designated strain JSM 073097, was isolated from a sea anemone collected from a tidal flat in the South China Sea. Phylogenetic analyses based on 16S rRNA gene sequences indicated that the new isolate was a member of the genus and was most closely related to HA-9, YIM 90004 and YIM 90010 (99.6, 98.5 and 98.1 % similarity, respectively). Phenotypic characteristics and chemotaxonomic data also indicated that strain JSM 073097 was a member of the genus s. The strain grew well on most of the media tested, producing white to yellow–white substrate mycelium and white aerial mycelium and straight to flexuous hyphae. The substrate mycelium was well developed and fragmented with age; the aerial mycelium produced long, straight to flexuous spore chains with non-motile, smooth-surfaced, rod-shaped spores. The strain grew in the presence of 1–15 % (w/v) total salts and at pH 6.0–10.5 and 20–35 °C; optimum growth occurred in the presence of 5–7 % (w/v) total salts and at pH 8.5 and 25 °C. Whole-cell hydrolysates of strain JSM 073097 contained -diaminopimelic acid and no diagnostic sugars. The predominant menaquinones were MK-10(H), MK-10(H) and MK-10(H). The major cellular fatty acids were iso-C, iso-C, anteiso-C and 10-methyl C. Polar lipids comprised diphosphatidylglycerol, phosphatidylcholine and phosphatidylglycerol. The DNA G+C content of strain JSM 073097 was 70.4 mol%. The combination of phylogenetic analysis, DNA–DNA relatedness data, phenotypic characteristics and chemotaxonomic data supported the suggestion that strain JSM 073097 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is JSM 073097 (=DSM 45168=KCTC 19473).


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Scanning electron micrograph of strain JSM 073097 . [ PDF] 199 KB



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