1887

Abstract

We previously isolated five strains of putative lactobacilli from the faeces of a thoroughbred horse (a 4-year-old male). Of the five strains, four were identified as members of existing species; however, sequence analysis of the 16S rRNA gene revealed that the fifth isolate, DI70, showed approximately 97 % identity (1325/1366 bp) with the type strain of . Therefore, we considered the possibility that DI70 represents a novel species of the genus . Cells of strain DI70 were Gram-stain-positive, catalase-negative, non-spore-forming, non-motile rods. In phylogenetic trees constructed on the basis of 16S rRNA gene sequences, strain DI70 formed a subcluster in the phylogenetic group and was closely related to , and . However, analysis of DNA–DNA relatedness showed that DI70 was genetically distinct from its phylogenetic relatives. The isolate also exhibited distinct biochemical and physiological characteristics when compared with its phylogenetic relatives. It required anaerobic conditions for growth on agar medium. The results indicate that isolate DI70 indeed represents a novel species of the genus , for which we propose the name sp. nov. The type strain is DI70 (=JCM 14600 =DSM 19284).

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2010-01-01
2019-10-19
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vol. , part 1, pp. 109 - 112

Phylogenetic relationships between strain DI70 and species in the phylogenetic group, as determined on the basis of sequence analysis of the 16S rRNA gene The trees were constructed using the maximum-parsimony method and the maximum-likelihood method. [PDF](37 KB)

DNA–DNA relatedness among strain DI70 , JCM 1002 , JCM 1185 and DSM 20557

NT, Not tested.



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