1887

International Journal of Systematic and Evolutionary Microbiology

Volume 59, Issue 12

sp. nov., isolated from rhizosphere soil of Free

Abstract

A novel actinomycete, strain S10, was isolated from rhizosphere soil of wild in Thinghir, Ouarzazate Province, Southern Morocco. The taxonomic status of this strain was established using a polyphasic approach. Strain S10 had white–grey aerial mycelium with long, spiral spore chains bearing smooth surfaced spores and produced a yellow diffusible pigment. Chemotaxonomic analyses showed that the cell wall of strain S10 contained -diaminopimelic acid and glycine. Phylogenetic analysis based on the almost complete 16S rRNA gene sequence indicated that strain S10 belonged to the Group I streptomycetes, branching off next to LMG 23016 from the group. DNA–DNA relatedness and phenotypic data distinguished strain S10 from the phylogenetically closest related type strains. It is therefore proposed that strain S10 (=CCMM B35=DSM 41919) represents the type strain of a novel species of the genus , for which the name sp. nov. is proposed.

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2009-12-01
2020-01-28
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References

  1. Bauer, A. W., Kirby, W. M., Sherris, J. C. & Turk, M. ( 1966; ). Antibiotic susceptibility testing by standard single disk method. Am J Clin Pathol 45, 493–496.
     [Google Scholar]
  2. Breccia, J. D., Castro, G. R., Baigori, M. D. & Siñeriz, F. ( 1995; ). Screening of xylanolytic bacteria using a colour plate method. J Appl Bacteriol 78, 469–472.[CrossRef]
    [Google Scholar]
  3. Cao, L., Qiu, Z., You, J., Tan, H. & Zhou, S. ( 2005; ). Isolation and characterization of endophytic streptomycete antagonists of Fusarium wilt pathogen from surface-sterilized banana roots. FEMS Microbiol Lett 247, 147–152.[CrossRef]
    [Google Scholar]
  4. Cashion, P., Holder-Franklin, M. A., McCully, J. & Franklin, M. ( 1977; ). A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81, 461–466.[CrossRef]
    [Google Scholar]
  5. Chun, J., Youn, H.-D., Yim, Y.-I., Lee, H., Kim, M. Y., Hah, Y. C. & Kang, S.-O. ( 1997; ). Streptomyces seoulensis sp. nov. Int J Syst Bacteriol 47, 492–498.[CrossRef]
    [Google Scholar]
  6. Chun, J., Lee, J.-H., Jung, Y., Kim, M., Kin, S., Kim, B. K. & Lim, Y.-W. ( 2007; ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57, 2259–2261.[CrossRef]
    [Google Scholar]
  7. Coenye, T., Falsen, E., Vancanneyt, M., Hoste, B., Govan, J. R. W., Kersters, K. & Vandamme, P. ( 1999; ). Classification of Alcaligenes faecalis-like isolates from the environment and human clinical samples as Ralstonia gilardii sp. nov. Int J Syst Bacteriol 49, 405–413.[CrossRef]
    [Google Scholar]
  8. Copping, L. G. & Menn, J. J. ( 2000; ). Biopesticides: a review of their action, applications and efficacy. Pest Manag Sci 56, 651–676.[CrossRef]
    [Google Scholar]
  9. De Ley, J., Cattoir, H. & Reynaerts, A. ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef]
    [Google Scholar]
  10. Donadio, S., Carrano, L., Brandi, L., Serina, S., Soffientini, A., Raimondi, E., Montanini, N., Sosio, M. & Gualerzi, C.-O. ( 2002; ). Targets and assays for discovering novel antibacterial agents. J Biotechnol 99, 175–185.[CrossRef]
    [Google Scholar]
  11. El-Tarabily, K. A. & Sivasithamparam, K. ( 2006; ). Non-streptomycete actinomycetes as biocontrol agents of soil-borne fungal plant pathogens and as plant growth promoters. Soil Biol Biochem 38, 1505–1520.[CrossRef]
    [Google Scholar]
  12. Felsenstein, J. ( 2005; ). phylip (phylogeny inference package) version 3.6. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle.
  13. Hopwood, D. A., Bibb, M. J., Chater, K. F., Kieser, T., Bruton, C. J., Kieser, H. M., Lydiate, D. J., Smith, C. P., Ward, J. M. & Schrempf, H. ( 1985; ). Genetic manipulation of Streptomyces: a laboratory manual. Norwich, UK: John Innes Foundation.
  14. Huson, D. H., Richter, D. C., Rausch, C., Dezulian, T., Franz, M. & Rupp, R. ( 2007; ). Dendroscope: An interactive viewer for large phylogenetic trees. BMC Bioinformatics 8, 460 [CrossRef]
    [Google Scholar]
  15. Huß, V. A. R., Festl, H. & Schleifer, K. H. ( 1983; ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4, 184–192.[CrossRef]
    [Google Scholar]
  16. Jones, J. ( 1949; ). Fresh isolates of actinomycetes in which the presence of sporogenous aerial mycelia is a fluctuating characteristic. J Bacteriol 57, 141–145.
     [Google Scholar]
  17. Klatte, S., Kroppenstedt, R. M. & Rainey, F. A. ( 1994; ). Rhodococcus opacus sp. nov., an unusual nutritionally versatile Rhodococcus species. Syst Appl Microbiol 17, 355–360.[CrossRef]
    [Google Scholar]
  18. Ko, W. H., Wang, I. T. & Ann, P. J. ( 2005; ). A simple method for detection of lipolytic microorganisms in soils. Soil Biol Biochem 37, 597–599.[CrossRef]
    [Google Scholar]
  19. Labeda, D. P., Lechevalier, M. P. & Testa, R. T. ( 1997; ). Streptomyces stramineus sp. nov., a new species of the verticillate streptomycetes. Int J Syst Bacteriol 47, 747–753.[CrossRef]
    [Google Scholar]
  20. Larkin, M. A., Blackshields, G., Brown, N. P., Chenna, R., McGettigan, P. A., McWilliam, H., Valentin, F., Wallace, I. M., Wilm, A. & other authors ( 2007; ). clustalw and clustalx version 2. Bioinformatics 23, 2947–2948.[CrossRef]
    [Google Scholar]
  21. Lazzarini, A., Cavaletti, L., Toppo, G. & Marinelli, F. ( 2000; ). Rare genera of actinomycetes as potential producers of new antibiotics. Antonie van Leeuwenhoek 78, 399–405.[CrossRef]
    [Google Scholar]
  22. Lechevalier, M. P. ( 1977; ). Lipids in bacterial taxonomy – a taxonomist's view. CRC Crit Rev Microbiol 5, 109–210.[CrossRef]
    [Google Scholar]
  23. Lechevalier, M. P. & Lechevalier, H. A. ( 1980; ). The chemotaxonomy of actinomycetes. In Actinomycetes Taxonomy (Special Publication of the Society for Industrial Microbiology no. A6), pp. 225–292. Edited by A. Dietz & D. W. Thayer. Arlington, Virginia Society of Industrial Microbiology.
  24. Liu, D., Coloe, S., Baird, R. & Pedersen, J. ( 2000; ). Rapid mini-preparation of fungal DNA for PCR. J Clin Microbiol 38, 471
    [Google Scholar]
  25. Locci, R. ( 1994; ). Actinomycetes as plant pathogens. Eur J Plant Pathol 100, 179–200.[CrossRef]
    [Google Scholar]
  26. Loqman, S., Ait Barka, E., Clément, C. & Ouhdouch, Y. ( 2009; ). Antagonistic actinomycetes from Moroccan soil to control the grapevine gray mold. World J Microbiol Biotechnol 25, 81–91.[CrossRef]
    [Google Scholar]
  27. Maskey, R. P., Li, F., Qin, S., Fiebig, H. H. & Laatsch, H. ( 2003; ). Chandrananimycins A∼C: production of novel anticancer antibiotics from a marine Actinomadura sp. isolate M048 by variation of medium composition and growth conditions. J Antibiot 56, 622–629.[CrossRef]
    [Google Scholar]
  28. Newman, D. J., Cragg, G. M. & Snader, K. M. ( 2003; ). Natural products as sources of new drugs over the period 1981–2002. J Nat Prod 66, 1022–1037.[CrossRef]
    [Google Scholar]
  29. Nonomura, H. ( 1974; ). Key for classification and identification of 485 species of the streptomycetes included in the ISP. J Ferment Technol 52, 78–92.
     [Google Scholar]
  30. Oakley, B., North, M., Franklin, J. F., Hedlund, B. F. & Staley, J. T. ( 2004; ). Diversity and distribution of Frankia strains symbiotic with Ceanothus in California. Appl Environ Microbiol 70, 6444–6452.[CrossRef]
    [Google Scholar]
  31. Olson, E. H. ( 1968; ). Actinomycetes isolation agar. Detroit, Michigan: Difco Laboratories.
  32. Ouhdouch, Y., Barakate, M. & Finance, C. ( 2001; ). Actinomycetes of Moroccan habitats: isolation and screening for antifungal activities. Eur J Soil Biol 37, 69–74.[CrossRef]
    [Google Scholar]
  33. Prauser, H. ( 1964; ). Aptness and application of colour for exact description of colours of Streptomyces. Z Allg Mikrobiol 10, 95–98.
     [Google Scholar]
  34. Sardi, P., Saracchi, M., Quaroni, S., Petrolini, B., Borgonovi, G. E. & Merli, S. ( 1992; ). Isolation of endophytic Streptomyces strains from surface-sterilized roots. Appl Environ Microbiol 58, 2691–2698.
     [Google Scholar]
  35. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  36. Shirling, E. B. & Gottlieb, D. ( 1966; ). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef]
    [Google Scholar]
  37. Tanaka, Y. & Omura, S. ( 1993; ). Agroactive compounds of microbial origin. Annu Rev Microbiol 47, 57–87.[CrossRef]
    [Google Scholar]
  38. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  39. Tresner, H. D., Hayes, J. A. & Backus, E. J. ( 1968; ). Differential tolerance of streptomycetes to sodium chloride as a taxonomic aid. Appl Microbiol 16, 1134–1138.
     [Google Scholar]
  40. Watve, M. G., Tickoo, R., Jog, M. M. & Bhole, B. D. ( 2001; ). How many antibiotics are produced by the genus Streptomyces? Arch Microbiol 176, 386–390.[CrossRef]
    [Google Scholar]
  41. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
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Streptomyces thinghirensis sp. nov., isolated from rhizosphere soil of Vitis vinifera
Int J Syst Evol Microbiol 59, 3063 (2009); https://doi.org/10.1099/ijs.0.008946-0
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Micrographs showing spore chains (left) and spore shape (right) of sp. nov. grown on ISP 2 for 14 days at 28°C.

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Micrographs showing spore chains (left) and spore shape (right) of sp. nov. grown on ISP 2 for 14 days at 28°C.

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Fatty acid pattern of strain 10 and closely related species of the genus . [ PDF] 61 KB

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