1887

Abstract

A Gram-negative, aerobic, non-spore-forming, halophilic bacterial strain, NTU-104, was isolated from the Szutsao saltern in southern Taiwan, which was previously used as salt production field. The novel isolate grew optimally at 35–40 °C, at pH 7.5–8.0 and in the presence of 5 % (w/v) NaCl. The major fatty acids were C, C 9, C 9, C 3-OH and C. The predominant quinone was Q-9. Phosphatidylglycerol, diphosphatidylglycerol and phosphatidylethanolamine were the predominant polar lipids. The DNA G+C content was 56.5 mol%. Phylogenetic analyses based on 16S rRNA gene sequences revealed the affiliation of the novel isolate to the genus . DNA–DNA hybridization results between strain NTU-104 and the type strains of the most closely related species, and , were 36.4 % and 33.2 %, respectively. On the basis of phenotypic, phylogenetic and genetic analyses, strain NTU-104 is considered to represent a novel species of the genus . The name sp. nov. is proposed, with strain NTU-104 (=BCRC 17809=CGMCC 1.7011=JCM 15751) as the type strain.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.008896-0
2009-10-01
2019-10-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/10/2605.html?itemId=/content/journal/ijsem/10.1099/ijs.0.008896-0&mimeType=html&fmt=ahah

References

  1. Antón, J., Oren, A., Benlloch, S., Rodríguez-Valera, F., Amann, R. & Rosselló-Mora, R. ( 2002; ). Salinibacter ruber gen. nov., sp. nov., a novel, extremely halophilic member of the Bacteria from saltern crystallizer ponds. Int J Syst Evol Microbiol 52, 485–491.
    [Google Scholar]
  2. Antunes, A., França, L., Rainey, F. A., Huber, R., Nobre, M. F., Edwards, K. J. & da Costa, M. S. ( 2007; ). Marinobacter salsuginis sp. nov., isolated from the brine–seawater interface of the Shaban Deep, Red Sea. Int J Syst Evol Microbiol 57, 1035–1040.[CrossRef]
    [Google Scholar]
  3. Azeredo, J. & Oliveira, R. ( 1996; ). A new method for precipitating bacterial exopolysaccharides. Biotechnol Tech 10, 341–344.
    [Google Scholar]
  4. Descheemaeker, P. & Swings, J. ( 1995; ). The application of fatty acid methyl ester analysis (FAME) for the identification of heterotrophic bacteria present in decaying Lede-stone of the St. Bavo Cathedral in Ghent. Sci Total Environ 167, 241–247.[CrossRef]
    [Google Scholar]
  5. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  6. Gauthier, M. J., Lafay, B., Christen, R., Fernandez, L., Acquaviva, M., Bonin, P. & Bertrand, J. C. ( 1992; ). Marinobacter hydrocarbonoclasticus gen. nov., sp. nov., a new, extremely halotolerant, hydrocarbon-degrading marine bacterium. Int J Syst Bacteriol 42, 568–576.[CrossRef]
    [Google Scholar]
  7. Gu, J., Cai, H., Yu, S.-L., Qu, R., Yin, B., Guo, Y.-F., Zhao, J.-Y. & Wu, X.-L. ( 2007; ). Marinobacter gudaonensis sp. nov., isolated from an oil-polluted saline soil in a Chinese oilfield. Int J Syst Evol Microbiol 57, 250–254.[CrossRef]
    [Google Scholar]
  8. Guo, B., Gu, J., Ye, Y.-G., Tang, Y.-Q., Kida, K. & Wu, X.-L. ( 2007; ). Marinobacter segnicrescens sp. nov., a moderate halophile isolated from benthic sediment of the South China Sea. Int J Syst Evol Microbiol 57, 1970–1974.[CrossRef]
    [Google Scholar]
  9. Heyrman, J., Mergaert, J., Denys, R. & Swings, J. ( 1999; ). The use of fatty acid methyl ester analysis (FAME) for the identification of heterotrophic bacteria present on three mural paintings showing severe damage by microorganisms. FEMS Microbiol Lett 181, 55–62.[CrossRef]
    [Google Scholar]
  10. Huo, Y. Y., Wang, C. S., Yang, J. Y., Wu, M. & Xu, X. W. ( 2008; ). Marinobacter mobilis sp. nov. and Marinobacter zhejiangensis sp. nov., halophilic bacteria isolated from the East China Sea. Int J Syst Evol Microbiol 58, 2885–2889.[CrossRef]
    [Google Scholar]
  11. Kim, B.-Y., Weon, H.-Y., Yoo, S.-H., Kim, J.-S., Kwon, S.-W., Stackebrandt, E. & Go, S.-J. ( 2006; ). Marinobacter koreensis sp. nov., isolated from sea sand in Korea. Int J Syst Evol Microbiol 56, 2653–2656.[CrossRef]
    [Google Scholar]
  12. Komagata, K. & Suzuki, K. ( 1987; ). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19, 161–207.
    [Google Scholar]
  13. Liebgott, P.-P., Casalot, L., Paillard, S., Lorquin, J. & Labat, M. ( 2006; ). Marinobacter vinifirmus sp. nov., a moderately halophilic bacterium isolated from a wine-barrel-decalcification wastewater. Int J Syst Evol Microbiol 56, 2511–2516.[CrossRef]
    [Google Scholar]
  14. Mas-Castellà, J. & Guerrero, R. ( 1995; ). Poly-β-hydroxyalkanoate determination in bacteria from aquatic samples. J Microbiol Methods 22, 151–164.[CrossRef]
    [Google Scholar]
  15. Mata, J. A., Martinez-Cánovas, J., Quesada, E. & Béjar, V. ( 2002; ). A detailed phenotypic characterisation of the type strains of Halomonas species. Syst Appl Microbiol 25, 360–375.[CrossRef]
    [Google Scholar]
  16. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  17. Montes, M. J., Bozal, N. & Mercadé, E. ( 2008; ). Marinobacter guineae sp. nov., a novel moderately halophilic bacterium from an Antarctic environment. Int J Syst Evol Microbiol 58, 1346–1349.[CrossRef]
    [Google Scholar]
  18. Shin, Y. K., Lee, J.-S., Chun, C. O., Kim, H.-J. & Park, Y.-H. ( 1996; ). Isoprenoid quinone profiles of the Leclercia adecarboxylata KCTC 1036T. J Microbiol Biotechnol 6, 68–69.
    [Google Scholar]
  19. Stackebrandt, E. & Liesack, W. ( 1993; ). Nucleic acids and classification. In Handbook of New Bacterial Systematics, pp. 152–189. Edited by M. Goodfellow & A. G. O'Donnell. London: Academic Press.
  20. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  21. Wang, C. Y., Chang, C. C., Ng, C. C., Chen, T. W. & Shyu, Y. T. ( 2008; ). Virgibacillus chiguensis sp. nov., a novel halophilic bacterium, isolated from Chigu, a previously commercial saltern located in southern Taiwan. Int J Syst Evol Microbiol 58, 341–345.[CrossRef]
    [Google Scholar]
  22. Xu, X. W., Wu, Y. H., Wang, C. S., Yang, J. Y., Oren, A. & Wu, M. ( 2008; ). Marinobacter pelagius sp. nov., a moderately halophilic bacterium. Int J Syst Evol Microbiol 58, 637–640.[CrossRef]
    [Google Scholar]
  23. Yoon, J.-H., Lee, M.-H., Kang, S.-H. & Oh, T.-K. ( 2007; ). Marinobacter salicampi sp. now. isolated from a marine solar saltern in Korea. Int J Syst Evol Microbiol 57, 2102–2105.[CrossRef]
    [Google Scholar]
  24. Zhang, D. C., Li, H. R., Xin, Y. H., Chi, Z. M., Zhou, P. J. & Yu, Y. ( 2008; ). Marinobacter psychrophilus sp. nov., a psychrophilic bacterium isolated from the Arctic. Int J Syst Evol Microbiol 58, 1463–1466.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.008896-0
Loading
/content/journal/ijsem/10.1099/ijs.0.008896-0
Loading

Data & Media loading...

Supplements

Cellular fatty acids (%) of strain NTU-104 and related species of the genus . [ PDF] 35 KB

PDF

Scanning electron photomicrograph of a cell of strain NTU-104 grown in basal broth. Bar, 1µm.

IMAGE

Two-dimensional TLC of the polar lipid of strain NTU-104 . PE, phosphatidylethanolamine; DPG, diphosphatidylglycerol; PG, phosphatidylglycerol.

IMAGE

Maximum-likelihood tree showing the phylogenetic topology of strain NTU-104 with other species of the genus and related taxa. [ PDF] 182 KB

PDF

Maximum-parsimony tree showing the phylogenetic topology of strain NTU-104 with other species of the genus and related taxa. [ PDF] 183 KB

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error