1887

Abstract

A Gram-negative, motile, rod-shaped, yellow-pigmented bacterium, strain UI2, was isolated from a hexachlorocyclohexane (HCH) dump site located in Ummari, in northern India. 16S rRNA gene sequence analysis revealed that strain UI2 belongs to the genus and showed highest 16S rRNA gene sequence similarity with IFO 15098 (98.6 %). DNA–DNA relatedness values between strain UI2 and IFO 15098 and other related strains were found to be less than 46 %. The major cellular fatty acids of strain UI2 were C 7 (28.3 %), C (14.4 %), 11-methyl C 7 (10.9 %), C 6 (9.6 %) and summed feature 3 (consisting of C 7 and/or C iso 2-OH; 14.7 %). The DNA G+C content of strain UI2 was 68 mol%. On the basis of DNA–DNA hybridization, phenotypic characteristics and phylogenetic analysis, strain UI2 is found to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is UI2 (=CCM 7428 =MTCC 8591).

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2010-04-01
2019-12-11
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Transmission electron micrograph of a negatively stained cell of strain UI2 , showing a subpolar flagellum. Bar, 0.5 µm.

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Two-dimensional TLC of polar lipids of strain UI2 . DPG, Diphosphatidylglycerol; GL, unknown glycolipid; L, unidentified lipid; PC, phosphatidylcholine; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; PME, phosphatidylmonomethylethanolamine; SGL, sphingoglycolipid.

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