1887

Abstract

A Gram-negative, aerobic, orange-coloured bacterium, designated strain GSW-2, was isolated from seawater sampled at a beach and its taxonomic status was established by using a polyphasic approach. Vegetative cells produced a prostheca and reproduced by budding. Mature vegetative cells were spherical, oval- or rod-shaped (0.6–1.1×1.2–1.3 μm) and both vegetative cells and buds produced one or more flagella. Cells grew well at 30 °C and at pH 8.1–9.1, and produced a non-diffusible pigment. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain GSW-2 was most closely related to the type strain of (97.5 % similarity), a member of the family , class . Chemotaxonomic characteristics supported the assignment of strain GSW-2 to the genus , including the dominant cellular fatty acids (summed feature 7 and C), isoprenoid quinone (mainly Q-10) and DNA G+C content (44.5 mol%). Levels of DNA–DNA relatedness between strain GSW-2 and DSM 5838 were 8.8–13.6 %. On the basis of phenotypic features and DNA–DNA hybridization data, strain GSW-2 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is GSW-2 (=DSM 19733=JCM 14974).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.008326-0
2009-09-01
2020-01-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/9/2264.html?itemId=/content/journal/ijsem/10.1099/ijs.0.008326-0&mimeType=html&fmt=ahah

References

  1. Abraham, W. R., Strömpl, C., Meyer, H., Lindholst, S., Moore, E. R., Christ, R., Vancanneyt, M., Tindall, B. J., Bennasar, A. & other authors ( 1999; ). Phylogeny and polyphasic taxonomy of Caulobacter species. Proposal of Maricaulis gen. nov. with Maricaulis maris (Poindexter) comb. nov. as the type species, and emended description of the genera Brevundimonas and Caulobacter. Int J Syst Bacteriol 49, 1053–1073.[CrossRef]
    [Google Scholar]
  2. Cashion, P., Holder-Franklin, M. A., McCully, J. & Franklin, M. ( 1977; ). A rapid method of base ratio determination of bacterial DNA. Anal Biochem 81, 461–466.[CrossRef]
    [Google Scholar]
  3. De Ley, J., Cattoir, H. & Reynaerts, A. ( 1970; ). The quantitative measurement of DNA hybridisation from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef]
    [Google Scholar]
  4. Felsenstein, J. ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef]
    [Google Scholar]
  5. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  6. Felsenstein, J. ( 1993; ). phylip (phylogeny inference package), version 3.5c. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  7. Fitch, W. M. ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef]
    [Google Scholar]
  8. Huß, V. A. R., Festl, H. & Schleifer, K. H. ( 1983; ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4, 184–192.[CrossRef]
    [Google Scholar]
  9. Jukes, T. H. & Cantor, C. R. ( 1969; ). Evolution of protein molecules. In Mammalian Protein Metabolism, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  10. Kroppenstedt, R. M. ( 1985; ). Fatty acid and menaquinone analysis of actinomycetes and related organisms. In Chemical Methods in Bacterial Systematics, pp. 173–199. Edited by M. Goodfellow & D. E. Minnikin. London: Academic Press.
  11. Lee, S. D. ( 2007; ). Tamlana crocina gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from beach sediment in Korea. Int J Syst Evol Microbiol 57, 764–769.[CrossRef]
    [Google Scholar]
  12. Lee, K., Lee, H. K., Choi, T. H. & Cho, J. C. ( 2007; ). Robiginitomaculum antarcticum gen. nov., sp. nov., a member of the family Hyphomonadaceae, from Antarctic seawater. Int J Syst Evol Microbiol 57, 2595–2599.[CrossRef]
    [Google Scholar]
  13. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  14. Minnikin, D. E., Patel, P. V., Alshamaony, L. & Goodfellow, M. ( 1977; ). Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 27, 104–117.[CrossRef]
    [Google Scholar]
  15. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A. & Parlett, J. H. ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef]
    [Google Scholar]
  16. Moore, R. L., Weiner, R. M. & Gebers, R. ( 1984; ). Genus Hyphomonas Pongratz 1957 nom. rev. emend., Hyphomonas polymorpha Pongratz 1957 nom. rev. emend., and Hyphomonas neptunium (Liefson 1964) comb. nov. emend. (Hyphomicrobium neptunium). Int J Syst Bacteriol 34, 71–73.[CrossRef]
    [Google Scholar]
  17. Pongratz, E. ( 1957; ). D'une bactérie pédiculée isolée d'un pus de sinus. Schweiz Z Pathol Bakteriol 20, 593–608.
    [Google Scholar]
  18. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  19. Schlesner, H., Bartels, C., Sittig, M., Dorsch, M. & Stackebrandt, E. ( 1990; ). Taxonomic and phylogenetic studies on a new taxon of budding, hyphal Proteobacteria, Hirschia baltica gen. nov., sp. nov. Int J Syst Bacteriol 40, 443–451.[CrossRef]
    [Google Scholar]
  20. Strömpl, C., Hold, G. N., Lunsdorf, H., Graham, J., Gallacher, S., Abraham, W.-R., Moore, E. R. B. & Timmis, K. N. ( 2003; ). Oceanicaulis alexandrii gen. nov., sp. nov., a novel stalked bacterium isolated from a culture of the dinoflagellate Alexandrium tamarense (Lebour) Balech. Int J Syst Evol Microbiol 53, 1901–1906.[CrossRef]
    [Google Scholar]
  21. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  22. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  23. Weiner, R. M., Melick, M., O'Neill, K. & Quintero, E. ( 2000; ). Hyphomonas adhaerens sp. nov., Hyphomonas johnsonii sp. nov. and Hyphomonas rosenbergii sp. nov., marine budding and prosthecate bacteria. Int J Syst Evol Microbiol 50, 459–469.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.008326-0
Loading
/content/journal/ijsem/10.1099/ijs.0.008326-0
Loading

Data & Media loading...

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error