1887

Abstract

A novel red-pigmented halophilic archaeon, strain A29, was isolated from shrimp jeotgal, a traditional salt-fermented food from Korea. This strain grows in the ranges 10–30 % (w/v) NaCl, 17–50 °C and pH 6.5–8.5, with optimal growth occurring at 15–20 % NaCl, 37–45 °C and pH 7.0–7.5. The isolate is Gram-negative and non-motile. Phylogenetic analysis, based on 16S rRNA gene sequences, showed that strain A29 is associated with the genus and closely related to the species (99.0 % similarity). However, DNA–DNA hybridization experiments revealed that the level of hybridization between strain A29 and related strains of is less than 70 %. The polar lipid fraction consists of phosphatidylglyerol (PG), phosphatidylglycerol phosphate methyl ester (PGP-Me) and mannose-2,6-disulfate(1-2)-glucose glycerol diether (S-DGD). The G+C content of genomic DNA of the type strain is 62.3 mol%. On the basis of this polyphasic taxonomic study, strain A29 should be placed in the genus as a novel species, for which the name sp. nov. is proposed. The type strain of the new species is A29 (=KCTC 4020=DSM 18794=JCM 14585=CECT 7218).

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2009-09-01
2019-12-07
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Phylogenetic tree, based on 16S rRNA gene sequences, showing the position of strain A29 with respect to other type species of the genus and other haloarchaea. The tree was generated by the minimum-evolution (top) and maximum-parsimony (bottom) methods. The numbers at the nodes indicate bootstrap values (1000 replications). Bar, 0.02 accumulated changes per nucleotide.

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Phylogenetic tree, based on 16S rRNA gene sequences, showing the position of strain A29 with respect to other type species of the genus and other haloarchaea. The tree was generated by the minimum-evolution (top) and maximum-parsimony (bottom) methods. The numbers at the nodes indicate bootstrap values (1000 replications). Bar, 0.02 accumulated changes per nucleotide.

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