1887

Abstract

As part of an enzyme-screening programme, an actinobacterium, strain HSM#10, was isolated from a sample collected from the base of a translucent quartz rock in Miers Valley, eastern Antarctica. The isolate produced branching vegetative mycelium that was characteristic of filamentous actinobacteria. The chemotaxonomic characteristics of the strain suggested that HSM#10 should be classified as a member of the genus . Furthermore, phylogenetic analysis based on 16S rRNA gene sequences showed that the strain was closely related to members of the genus , which supports the classification of this strain within the family . Phenotypic and phylogenetic results allowed strain HSM#10 to be differentiated from known streptomycetes. DNA–DNA hybridization data also showed that strain HSM#10 could be differentiated from its nearest phylogenetic neighbours DSM 40420 (53.55±3.15 % DNA relatedness), DSM 40431 (38.75±2.75 %), DSM 40150 (30.7±2.90 %) and DSM 41800 (33.9±0.10 %). Therefore, the name sp. nov. is proposed, with HSM#10 (=DSM 41950 =NRRL B-24669) as the type strain.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.007971-0
2009-08-01
2019-12-05
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/8/2032.html?itemId=/content/journal/ijsem/10.1099/ijs.0.007971-0&mimeType=html&fmt=ahah

References

  1. Altschul, S. F., Madden, T. L., Schäffer, A. A., Zhang, J., Zhang, Z., Miller, W. & Lipman, D. J. ( 1997; ). Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25, 3389–3402.[CrossRef]
    [Google Scholar]
  2. Atlas, R. M. ( 2004; ). Handbook of Microbiological Media, 3rd edn. Edited by L. C. Parks. Boca Raton, FL: CRC Press.
  3. Cashion, P., Holder-Franklin, M. A., McCully, J. & Franklin, M. ( 1977; ). A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81, 461–466.[CrossRef]
    [Google Scholar]
  4. Chun, J., Lee, J.-H., Jung, Y., Kim, M., Kim, S., Kim, B. K. & Lim, Y.-W. ( 2007; ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57, 2259–2261.[CrossRef]
    [Google Scholar]
  5. Cockell, C. S. & Stokes, M. D. ( 2004; ). Ecology: widespread colonization by polar hypoliths. Nature 431, 414 [CrossRef]
    [Google Scholar]
  6. Cook, A. E. & Meyers, P. R. ( 2003; ). Rapid identification of filamentous actinomycetes to the genus level using genus-specific 16S rRNA gene restriction fragment patterns. Int J Syst Evol Microbiol 53, 1907–1915.[CrossRef]
    [Google Scholar]
  7. De Ley, J., Cattoir, H. & Reynaerts, A. ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef]
    [Google Scholar]
  8. Hasegawa, T., Takizawa, M. & Tanida, S. ( 1983; ). A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29, 319–322.[CrossRef]
    [Google Scholar]
  9. Huß, V. A. R., Festl, H. & Schleifer, K. H. ( 1983; ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4, 184–192.[CrossRef]
    [Google Scholar]
  10. Keswani, J. & Whitman, W. B. ( 2001; ). Relationship of 16S rRNA sequence similarity to DNA hybridization in prokaryotes. Int J Syst Evol Microbiol 51, 667–678.
    [Google Scholar]
  11. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  12. Lechevalier, M. P. & Lechevalier, H. A. ( 1970; ). Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20, 435–443.[CrossRef]
    [Google Scholar]
  13. Locci, R. ( 1989; ). Streptomyces and related genera. In Bergey's Manual of Systematic Bacteriology, vol. 4, pp. 2451–2508. Edited by S. T. Williams, M. E. Sharpe & J. G. Holt. Baltimore: Williams & Wilkins.
  14. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  15. Shirling, E. B. & Gottlieb, D. ( 1966; ). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef]
    [Google Scholar]
  16. Stackebrandt, E. & Ebers, J. ( 2006; ). Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 33, 152–155.
    [Google Scholar]
  17. Takahashi, K. & Nei, M. ( 2000; ). Efficiencies of fast algorithms of phylogenetic inference under the criteria of maximum parsimony, minimum evolution, and maximum likelihood when a large number of sequences are used. Mol Biol Evol 17, 1251–1258.[CrossRef]
    [Google Scholar]
  18. Thomas, D. N. ( 2005; ). Photosynthetic microbes in freezing deserts. Trends Microbiol 13, 87–88.[CrossRef]
    [Google Scholar]
  19. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.007971-0
Loading
/content/journal/ijsem/10.1099/ijs.0.007971-0
Loading

Data & Media loading...

Supplements

vol. , part 8, pp. 2032 - 2035

Comparison of the growth characteristics of strain HSM#10 , NRRL B-12347 (=DSM 40420 ) and NRRL B-12365 (=DSM 40431 ) on different agar media. [PDF](46 KB)



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error