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As part of an enzyme-screening programme, an actinobacterium, strain HSM#10T, was isolated from a sample collected from the base of a translucent quartz rock in Miers Valley, eastern Antarctica. The isolate produced branching vegetative mycelium that was characteristic of filamentous actinobacteria. The chemotaxonomic characteristics of the strain suggested that HSM#10T should be classified as a member of the genus Streptomyces. Furthermore, phylogenetic analysis based on 16S rRNA gene sequences showed that the strain was closely related to members of the genus Streptomyces, which supports the classification of this strain within the family Streptomycetaceae. Phenotypic and phylogenetic results allowed strain HSM#10T to be differentiated from known streptomycetes. DNA–DNA hybridization data also showed that strain HSM#10T could be differentiated from its nearest phylogenetic neighbours Streptomyces chryseus DSM 40420T (53.55±3.15 % DNA relatedness), Streptomyces helvaticus DSM 40431T (38.75±2.75 %), Streptomyces flavidovirens DSM 40150T (30.7±2.90 %) and Streptomyces albidochromogenes DSM 41800T (33.9±0.10 %). Therefore, the name Streptomyces hypolithicus sp. nov. is proposed, with HSM#10T (=DSM 41950T =NRRL B-24669T) as the type strain.
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International Journal of Systematic and Evolutionary Microbiology vol. 59 , part 8, pp. 2032 - 2035
Supplementary Table S1. Comparison of the growth characteristics of strain HSM#10 T, Streptomyces chryseus NRRL B-12347 T(=DSM 40420 T) and S. helvaticus NRRL B-12365 T(=DSM 40431 T) on different agar media. [PDF](46 KB)