1887

Abstract

A Gram-staining-negative, rod-shaped and non-motile bacterium, designated CL-SS4, was isolated from crude-oil-contaminated seawater from the west coast of Korea and was investigated by means of a polyphasic taxonomic approach. Strain CL-SS4 grew optimally at 25–30 °C, at pH 6.8–7.7 and in the presence of 2–3 % sea salts. The major fatty acids were iso-C 3-OH, iso-C and iso-C G. The major isoprenoid quinone was MK-6. The DNA G+C content was 50.7 mol%. Analysis of the 16S rRNA gene sequence of strain CL-SS4 revealed that it was a member of the genus , sharing 95.7–96.9 % sequence similarity with the type strains of species and being most closely related to SW-63. Phylogenetic analyses based on 16S rRNA gene sequences confirmed that strain CL-SS4 formed a deep lineage related to the genus . Strain CL-SS4 was distinguishable from recognized species on the basis of differential phenotypic characteristics and DNA G+C content. Based on these results, it is proposed that strain CL-SS4 represents a novel species of the genus , for which the name sp. nov. is proposed. An emended description of the genus is also proposed. The type strain is CL-SS4 (=KCCM 90075=JCM 15563).

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2009-08-01
2019-10-21
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