1887

Abstract

A Gram-positive-staining, facultatively anaerobic bacterial strain, CTT-37, was isolated from a marine sediment sample collected from Tottori city, located on the shore of the Sea of Japan. A 16S rRNA gene sequence comparison indicated that the isolate represents a novel clade that clusters with members of the families and . Strain CTT-37 shared maximum 16S rRNA gene sequence similarity of 96.4 % with DSM 14281 and 96.2 % with DSM 43852. The DNA–DNA hybridization value between strain CTT-37 and JCM 7350 was 10–12 %. The following chemotaxonomic characteristics of strain CTT-37 were markedly different from those of strains in the genus . The cell wall contained -serine in the peptidoglycan interpeptide bridge. The predominant menaquinone was MK-9 (H); other quinones detected were MK-9 and MK-9(H). The only polar lipid was phosphatidylglycerol and the G+C content of the DNA was 70 mol%. Differences in phenotypic characteristics and large phylogenetic distances between strain CTT-37 and all members of the genus supported the classification of CTT-37 within a new genus and species, for which the name gen. nov., sp. nov. is proposed. The type strain of is CTT-37 (=NBRC 104352 =DSM 21750).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.007666-0
2009-08-01
2024-12-12
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/8/2094.html?itemId=/content/journal/ijsem/10.1099/ijs.0.007666-0&mimeType=html&fmt=ahah

References

  1. Adachi, J. & Hasegawa, M.(1996).MOLPHY version 2.3 – Programs for molecular phylogenetics based on maximum likelihood. Computer Science Monograph no. 28. Tokyo: Institute of Statistical Mathematics.
  2. Altschul, S. F., Gish, W., Miller, W., Myers, E. W. & Lipman, D. J.(1990). Basic local alignment search tool. J Mol Biol 215, 403–410.[CrossRef] [Google Scholar]
  3. Bergey, D. H., Harrison, F. C., Breed, R. S., Hammer, B. W. & Huntoon, F. M.(1923).Bergey's Manual of Determinative Bacteriology. Baltimore: Williams & Wilkins.
  4. Brosius, J., Palmer, M. L., Kennedy, P. J. & Noller, H. F.(1978). Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. Proc Natl Acad Sci U S A 75, 4801–4805.[CrossRef] [Google Scholar]
  5. Buck, J. D.(1982). Nonstaining (KOH) method for determination of Gram reactions of marine bacteria. Appl Environ Microbiol 44, 992–993. [Google Scholar]
  6. Dasman, Kajiyama, S.-I., Kawasaki, H., Yagi, M., Seki, T., Fukusaki, E.-I. & Kobayashi, A.(2002).Paenibacillus glycanilyticus sp. nov., a novel species that degrades heteropolysaccharide produced by the cyanobacterium Nostoc commune. Int J Syst Evol Microbiol 52, 1669–1674.[CrossRef] [Google Scholar]
  7. Ezaki, T., Hashimoto, Y. & Yabuuchi, E.(1989). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef] [Google Scholar]
  8. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  9. Kang, M.-S., Im, W.-T., Jung, H.-M., Kim, M. K., Goodfellow, M., Kim, K. K., Yang, H.-C., An, D.-S. & Lee, S.-T.(2007).Cellulomonas composti sp. nov., a cellulolytic bacterium isolated from cattle farm compost. Int J Syst Evol Microbiol 57, 1256–1260.[CrossRef] [Google Scholar]
  10. Khan, S. T., Nakagawa, Y. & Harayama, S.(2007).Sediminibacter furfurosus gen. nov., sp. nov. and Gilvibacter sediminis gen. nov., sp. nov., novel members of the family Flavobacteriaceae. Int J Syst Evol Microbiol 57, 265–269.[CrossRef] [Google Scholar]
  11. La Scola, B., Fenollar, F., Fournier, P. E., Altwegg, M., Mallet, M. N. & Raoult, D.(2001). Description of Tropheryma whipplei gen. nov., sp. nov., the Whipple's disease bacillus. Int J Syst Evol Microbiol 51, 1471–1479. [Google Scholar]
  12. Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef] [Google Scholar]
  13. Minamisawa, K.(1990). Division of rhizobitoxine-producing and hydrogen-uptake positive strains of Bradyrhizobium japonicum by nifDKE sequence divergence. Plant Cell Physiol 31, 81–89. [Google Scholar]
  14. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A. & Parlett, J. H.(1984). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef] [Google Scholar]
  15. Nakagawa, Y. & Yamasato, K.(1993). Phylogenetic diversity of the genus Cytophaga revealed by 16S rRNA sequencing and menaquinone analysis. J Gen Microbiol 139, 1155–1161.[CrossRef] [Google Scholar]
  16. Needleman, S. B. & Wunsch, C. D.(1970). A general method applicable to the search for similarities in the amino acid sequence of two proteins. J Mol Biol 48, 443–453.[CrossRef] [Google Scholar]
  17. Prauser, H., Lechevalier, M. P. & Lechevalier, H. A.(1970). Description of Oerskovia gen. n. to harbor Ørskov's motile Nocardia. Appl Microbiol 19, 534 [Google Scholar]
  18. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  19. Sasser, M.(1990).Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  20. Shirling, E. B. & Gottlieb, D.(1966). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef] [Google Scholar]
  21. Smibert, R. M. & Krieg, N. R.(1981). General characterization. In Manual of Methods for General Bacteriology, pp. 409–443. Edited by P. Gerhardt, R. G. E. Murray, R. N. Costilow, E. W. Nester, W. A. Wood, N. R. Krieg & G. B. Phillips. Washington, DC: American Society for Microbiology.
  22. Stackebrandt, E. & Prauser, H.(1991). The family Cellulomonadaceae. In The Prokaryotes, 2nd edn, vol. 2, pp. 1323–1345. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K. H. Schleifer. New York: Springer.
  23. Stackebrandt, E., Häringer, M. & Schleifer, K. H.(1980). Molecular genetic evidence for the transfer of Oerskovia species into the genus Cellulomonas. Arch Microbiol 127, 179–185.[CrossRef] [Google Scholar]
  24. Stackebrandt, E., Seiler, H. & Schleifer, K. H.(1982). Union of the genera Cellulomonas Bergey et al. and Oerskovia Prauser et al. in a redefined genus Cellulomonas. Zentralbl Bakteriol Hyg Abt 1 Orig C 3, 401–409. [Google Scholar]
  25. Stackebrandt, E., Rainey, F. A. & Ward-Rainey, N. L.(1997). Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47, 479–491.[CrossRef] [Google Scholar]
  26. Stackebrandt, E., Breymann, S., Steiner, U., Prauser, H., Weiss, N. & Schumann, P.(2002). Re-evaluation of the status of the genus Oerskovia, reclassification of Promicromonospora enterophila (Jáger et al. 1983) as Oerskovia enterophila comb. nov. and description of Oerskovia jenensis sp. nov. and Oerskovia paurometabola sp. nov. Int J Syst Evol Microbiol 52, 1105–1111.[CrossRef] [Google Scholar]
  27. Tamura, T., Nakagaito, Y., Nishii, T., Hasegawa, T., Stackebrandt, E. & Yokota, A.(1994). A new genus of the order Actinomycetales, Couchioplanes gen. nov., with descriptions of Couchioplanes caeruleus (Horan and Brodsky 1986) comb. nov. and Couchioplanes caeruleus subsp. azureus subsp. nov. Int J Syst Bacteriol 44, 193–203.[CrossRef] [Google Scholar]
  28. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  29. Yi, H., Schumann, P. & Chun, J.(2007).Demequina aestuarii gen. nov., sp. nov., a novel actinomycete of the suborder Micrococcineae, and reclassification of Cellulomonas fermentans Bagnara et al. 1985 as Actinotalea fermentans gen. nov., comb. nov. Int J Syst Evol Microbiol 57, 151–156.[CrossRef] [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.007666-0
Loading
/content/journal/ijsem/10.1099/ijs.0.007666-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error