1887

Abstract

Five strains of bacteria isolated from nodules of and in Yunnan Province of China were classified within the genus in the class . The highest degree of 16S rRNA gene sequence similarity was determined to be to LMG 6125 (99.7 %) and UPM-Ca7 (99.7 %). Polyphasic taxonomic methods including SDS-PAGE of whole-cell soluble proteins, comparative housekeeping sequence analysis of , and , fatty acid profiles and a series of phenotypic and physiological tests allowed us to cluster the five strains into a coherent group while differentiating them from all previously established species. The DNA–DNA relatedness between the representative strain CCBAU 65327 and the type strains of and was 26.5 and 23.4 %, respectively, clearly indicating that strain CCBAU 65327 represents a novel species for which we propose the name sp. nov. Strain CCBAU 65327 (=LMG 24762 =HAMBI 3050) is designated as the type strain, and could nodulate , , , , and in cross-nodulation tests.

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2009-12-01
2020-01-22
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vol. , part 12, pp. 3012 - 3018

[ PDF] (195KB), containing:

Dendrogram based upon SDS-PAGE of whole-cell soluble proteins.

Comparison of partial (a) and (b) gene phylogenies, showing that the novel group has close relationships with the type strains of , , and and is distantly related to the other species of the genus .

Sequence similarities of 16S rRNA gene, , , , and and DNA relatedness among novel strain CCBAU 65327 and related strains.

Cellular fatty acid compositions of strain CCBAU 65327 and previously reported species of the genus .



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