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Abstract

A Gram-positive, non-motile, white-pigmented, short rod actinobacterium, designated YIM 90734, was isolated from a saline soil sample collected from Ganjiahu Suosuo Forest National Nature Reserve in Xinjiang province, north-west China, and its taxonomic position was investigated by using a polyphasic approach. Strain YIM 90734 grew optimally at 28–37 °C and pH 6.0–8.0 and in 5 % (w/v) NaCl. The peptidoglycan type was A4, -Lys–-Ala–-Glu and tyvelose and mannose were the major cell-wall sugars. The predominant menaquinones were MK-10 and MK-9. Major cellular fatty acids (>10 % of total) were anteiso-C and anteiso-C. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, one unknown phospholipid and two unknown glycolipids. The DNA G+C content was 70.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM 90734 belonged to the genus . The 16S rRNA gene sequence similarity between strain YIM 90734 and the type strain of the only recognized species, , was 97.7 %. However, the level of DNA–DNA relatedness of the two strains was 41.4 %. The DNA–DNA relatedness data and differential phenotypic properties, together with the phylogenetic distinctiveness, demonstrated that strain YIM 90734 could be differentiated from . On the basis of the data presented, strain YIM 90734 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is YIM 90734 (=KCTC 19375=DSM 21143). The description of the genus has also been emended.

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2009-08-01
2020-01-23
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Chromatograms of OPA-derivatization samples. [PDF](25 KB)

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Chromatograms of PMP-derivatization samples. [PDF](25 KB)

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Cell-wall sugar compositions of strain YIM 90734 ( sp. nov.) and YIM 70185 . [PDF](11 KB)

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