1887

Abstract

A novel Gram-negative and facultative anaerobic strain, designated MS1, was isolated from gajami sikhae, a traditional fermented food in Korea made from flatfish. Strain MS1 was motile, rod-shaped and oxidase- and catalase-positive, and required 1–2 % (w/v) NaCl for growth. Growth occurred at temperatures ranging from 4 to 40 °C and the pH range for optimal growth was pH 6.5–9.0. Strain MS1 was capable of reducing trimethylamine oxide, nitrate and thiosulfate. Phylogenetic analysis placed strain MS1 within the genus . Phylogenetic analysis based on 16S rRNA gene sequences showed that strain MS1 was related closely to B11 (98.67 % similarity) and CCUG 30811 (98.04 % similarity). However, DNA–DNA reassociation experiments between strain MS1 and reference strains showed relatedness values <70 % (42.6 and 14.8 % with B11 and CCUG 30811, respectively). Genotypic, physiological and biochemical analyses allowed the differentiation of strain MS1 from type strains of species belonging to the genus . Therefore, we propose that strain MS1 (=KCTC 22429 =JCM 15561) is assigned to a novel species, sp. nov.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.007260-0
2009-09-01
2019-10-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/9/2313.html?itemId=/content/journal/ijsem/10.1099/ijs.0.007260-0&mimeType=html&fmt=ahah

References

  1. Baker, G. C., Smith, J. J. & Cowan, D. A. ( 2003; ). Review and re-analysis of domain-specific 16S primers. J Microbiol Methods 55, 541–555.[CrossRef]
    [Google Scholar]
  2. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  3. Felsenstein, J. ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef]
    [Google Scholar]
  4. Fonnesbech Vogel, B. F., Venkateswaran, K., Christensen, H., Falsen, E., Christiansen, G. & Gram, L. ( 2000; ). Polyphasic taxonomic approach in the description of Alishewanella fetalis gen. nov., sp. nov., isolated from a human foetus. Int J Syst Evol Microbiol 50, 1133–1142.[CrossRef]
    [Google Scholar]
  5. Gonzalez, J. M. & Saiz-Jimenez, C. ( 2002; ). A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ Microbiol 4, 770–773.[CrossRef]
    [Google Scholar]
  6. Gram, H. ( 1884; ). Über die isolierte Färbung der Schizomyceten in Schnitt- und Trockenpräparaten. Fortschr Med 2, 185–189 (in German).
    [Google Scholar]
  7. Heimbrook, M. E., Wang, W. L. & Campbell, G. ( 1989; ). Staining bacterial flagella easily. J Clin Microbiol 27, 2612–2615.
    [Google Scholar]
  8. Hirayama, H., Tamaoka, J. & Horikoshi, K. ( 1996; ). Improved immobilization of DNA to microwell plates for DNA–DNA hybridization. Nucleic Acids Res 24, 4098–4099.[CrossRef]
    [Google Scholar]
  9. Kluge, A. G. & Farris, F. S. ( 1969; ). Quantitative phyletics and the evolution of anurans. Syst Zool 18, 1–32.[CrossRef]
    [Google Scholar]
  10. Loffler, F. E., Sanford, R. A. & Tiedje, J. M. ( 1996; ). Initial characterization of a reductive dehalogenase from desulfitobacterium chlororespirans Co23. Appl Environ Microbiol 62, 3809–3813.
    [Google Scholar]
  11. MIDI ( 1999; ). Sherlock Microbial Identification System Operating Manual, version 3.0. Newark, DE: MIDI, Inc.
  12. Roh, S. W., Nam, Y.-D., Chang, H.-W., Kim, K.-H., Kim, M.-S., Oh, H.-M. & Bae, J.-W. ( 2009; ). Alishewanella aestuarii sp. nov., isolated from tidal flat sediment, and emended description of the genus Alishewanella. Int J Syst Evol Microbiol 59, 421–424.[CrossRef]
    [Google Scholar]
  13. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  14. Sambrook, J., Fritsch, E. F. & Maniatis, T. ( 1989; ). Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  15. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  16. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  17. Tamura, K., Dudley, J., Nei, M. & Kumar, S. ( 2007; ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24, 1596–1599.[CrossRef]
    [Google Scholar]
  18. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  19. Wolin, E. A., Wolin, M. J. & Wolfe, R. S. ( 1963; ). Formation of methane by bacterial extracts. J Biol Chem 238, 2882–2886.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.007260-0
Loading
/content/journal/ijsem/10.1099/ijs.0.007260-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error