Two novel -like bacteria, strains KC 40 and KC 65, were isolated from a marine crustacean specimen collected from the Sea of Japan, and were characterized by using a polyphasic approach. Strains were selected on the basis of their ability to produce black–brown diffusible pigments on commonly used organic media, which appears to be a unique characteristic of recognized members of the genus . Phylogenetic analyses based on both 16S rRNA and gene sequences showed that the novel isolates formed a separate cluster within the genus . Strains KC 40 and KC 65 shared highest levels of 16S rRNA gene sequence similarity with DSM 14009 (98.0 %), CCUG 46240 (97.9 %), JG-219 (97.9 %), Iso-46 (97.8 %), SW-210 (97.6 %), SW-242 (97.6 %) and 88/2-7 (97.6 %). DNA–DNA hybridization experiments revealed 84 % DNA–DNA relatedness between strains KC 40 and KC 65 but much lower levels of relatedness (7–35 %) between the novel strains and the type strains of recognized species, confirming their assignment to a single novel species of the genus . The two novel strains could be distinguished from recognized species of the genus based on a combination of physiological and biochemical characteristics. On the basis of phenotypic and molecular properties, strains KC 40 and KC 65 are considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is KC 40 (=KMM 3954=NRIC 0746=JCM 15525).


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Maximum-parsimony phylogenetic tree based on 16S rRNA gene sequences showing the relationships of strains KC 40 and KC 65 and species. [ PDF] 36 KB


Maximum-parsimony phylogenetic tree based on gene sequences showing strains KC 40 and KC 65 and species. [ PDF] 37 KB


Cellular fatty acid composition (%) of strains KC 40 and KC 65. [ PDF] 44 KB


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