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Two novel Psychrobacter-like bacteria, strains KC 40T and KC 65, were isolated from a marine crustacean specimen collected from the Sea of Japan, and were characterized by using a polyphasic approach. Strains were selected on the basis of their ability to produce black–brown diffusible pigments on commonly used organic media, which appears to be a unique characteristic of recognized members of the genus Psychrobacter. Phylogenetic analyses based on both 16S rRNA and gyrB gene sequences showed that the novel isolates formed a separate cluster within the genus Psychrobacter. Strains KC 40T and KC 65 shared highest levels of 16S rRNA gene sequence similarity with Psychrobacter urativorans DSM 14009T (98.0 %), Psychrobacter pulmonis CCUG 46240T (97.9 %), Psychrobacter cibarius JG-219T (97.9 %), Psychrobacter faecalis Iso-46T (97.8 %), Psychrobacter aquimaris SW-210T (97.6 %), Psychrobacter namhaensis SW-242T (97.6 %) and Psychrobacter nivimaris 88/2-7T (97.6 %). DNA–DNA hybridization experiments revealed 84 % DNA–DNA relatedness between strains KC 40T and KC 65 but much lower levels of relatedness (7–35 %) between the novel strains and the type strains of recognized Psychrobacter species, confirming their assignment to a single novel species of the genus Psychrobacter. The two novel strains could be distinguished from recognized species of the genus Psychrobacter based on a combination of physiological and biochemical characteristics. On the basis of phenotypic and molecular properties, strains KC 40T and KC 65 are considered to represent a novel species of the genus Psychrobacter, for which the name Psychrobacter fulvigenes sp. nov. is proposed. The type strain is KC 40T (=KMM 3954T=NRIC 0746T=JCM 15525T).
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