1887

Abstract

The taxonomic positions of actinomycete strains ID03-0915 and ID03-0825, isolated from soil on the Indonesian island of Bali, were examined using a polyphasic taxonomic approach. The morphological and chemotaxonomic characteristics of these organisms are typical of the genus . Phylogenetic analyses performed using almost-complete 16S rRNA gene sequences demonstrated that the strains were closely related to and . However, DNA–DNA hybridization and phenotypic characteristics revealed that the strains differed from known species. Therefore, we conclude that strains ID03-0915 and ID03-0825 (=BTCC B-563) represent a novel species of the genus , for which we propose the name sp. nov. The type strain is strain ID03-0915 (=BTCC B-608 =NBRC 104276).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.007179-0
2009-09-01
2020-01-26
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/9/2158.html?itemId=/content/journal/ijsem/10.1099/ijs.0.007179-0&mimeType=html&fmt=ahah

References

  1. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  2. Felsenstein, J. ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef]
    [Google Scholar]
  3. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  4. Fitch, W. M. ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef]
    [Google Scholar]
  5. Hain, T., Ward-Rainey, N., Kroppenstedt, R. M., Stackebrandt, E. & Rainey, F. A. ( 1997; ). Discrimination of Streptomyces albidoflavus strains based on the size and number of 16S–23S ribosomal DNA intergenic spacers. Int J Syst Bacteriol 47, 202–206.[CrossRef]
    [Google Scholar]
  6. Hasegawa, T., Takizawa, M. & Tanida, S. ( 1983; ). A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29, 319–322.[CrossRef]
    [Google Scholar]
  7. Hatano, K., Nishii, T. & Kasai, H. ( 2003; ). Taxonomic re-evaluation of whorl-forming Streptomyces (formerly Streptoverticillium) species by using phenotypes, DNA–DNA hybridization and sequence of gyrB, and proposal of Streptomyces luteireticuli (ex Katoh and Arai 1957) corrig., sp. nov., nom. rev. Int J Syst Evol Microbiol 53, 1519–1529.[CrossRef]
    [Google Scholar]
  8. Hayakawa, M. & Nonomura, H. ( 1987; ). Humic acid-vitamin agar, a new medium for selective isolation of soil actinomycetes. J Ferment Technol 65, 501–509.[CrossRef]
    [Google Scholar]
  9. Hayakawa, M. & Nonomura, H. ( 1989; ). A new method for the intensive isolation of actinomycetes from soil. Actinomycetologica 3, 95–104.[CrossRef]
    [Google Scholar]
  10. Kämpfer, P. & Kroppenstedt, R. M. ( 1996; ). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42, 989–1005.[CrossRef]
    [Google Scholar]
  11. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary genetics analyses and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  12. Labeda, D. P. ( 1993; ). DNA relatedness among strains of the Streptomyces lavendulae phenotypic cluster group. Int J Syst Bacteriol 43, 822–825.[CrossRef]
    [Google Scholar]
  13. Labeda, D. P. ( 1996; ). DNA relatedness among verticil-forming Streptomyces species (formerly Streptoverticillium species). Int J Syst Bacteriol 46, 699–703.[CrossRef]
    [Google Scholar]
  14. Labeda, D. P. ( 1998; ). DNA relatedness among the Streptomyces fulvissimus and Streptomyces griseoviridis phenotypic cluster groups. Int J Syst Bacteriol 48, 829–832.[CrossRef]
    [Google Scholar]
  15. Lechevalier, M. P. & Lechevalier, H. A. ( 1980; ). The chemotaxonomy of actinomycetes. In Actinomycete Taxonomy (Special Publication no. 6), pp. 277–284. Edited by A. Dietz & D. W. Thayer. Arlington, VA: Society of Industrial Microbiology.
  16. Manfio, G. P., Zakrzewska-Czerwinska, J., Atalan, E. & Goodfellow, M. ( 1995; ). Towards minimal standards for the description of Streptomyces species. Biotekhnologia 7–8, 242–253.
    [Google Scholar]
  17. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A. & Parlett, J. H. ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef]
    [Google Scholar]
  18. Saito, H. & Miura, K. ( 1963; ). Preparation of transforming deoxyribonucleic acid by phenol treatment. Biochim Biophys Acta 72, 619–629.[CrossRef]
    [Google Scholar]
  19. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  20. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  21. Schaal, K. P. ( 1985; ). Identification of clinically significant actinomycetes and related bacteria using chemical techniques. In Chemical Methods in Bacterial Systematics, pp. 359–381. Edited by M. Goodfellow & D. E. Minnikin. London: Academic Press.
  22. Shirling, E. B. & Gottlieb, D. ( 1966; ). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef]
    [Google Scholar]
  23. Tamura, T. & Hatano, K. ( 2001; ). Phylogenetic analyses of the genus Actinoplanes and transfer of Actinoplanes minutisporangius Ruan et al. 1986 and ‘Actinoplanes aurantiacus’ to Cryptosporangium minutisporangium comb. nov. and Cryptosporangium aurantiacum sp. nov. Int J Syst Evol Microbiol 51, 2119–2125.[CrossRef]
    [Google Scholar]
  24. Tamura, T., Nakagaito, Y., Nishii, T., Hasegawa, T., Stackebrandt, E. & Yokota, A. ( 1994; ). A new genus of the order Actinomycetales, Couchioplanes gen. nov., with descriptions of Couchioplanes caeruleus (Horan and Brodsky 1986) comb. nov. and Couchioplanes caeruleus subsp. azureus subsp. nov. Int J Syst Bacteriol 44, 193–203.[CrossRef]
    [Google Scholar]
  25. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analyses tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  26. Waksman, S. A. & Henrici, A. T. ( 1943; ). The nomenclature and classification of the actinomycetes. J Bacteriol 46, 337–341.
    [Google Scholar]
  27. Williams, S. T., Goodfellow, M. & Alderson, G. ( 1989; ). Genus Streptomyces Waksman and Henrici, 1943, 339AL. In Bergey's Manual of Systematic Bacteriology, vol. 4, pp. 2452–2492. Edited by S. T. Williams, M. E. Sharpe & J. G. Holt. Baltimore: Williams & Wilkins.
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.007179-0
Loading
/content/journal/ijsem/10.1099/ijs.0.007179-0
Loading

Data & Media loading...

Supplements

vol. , part 9, pp. 2158 - 2161

Growth and culture characteristics of strains ID03-0915 and ID03-0825. [PDF](47 KB)



PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error