Eight bacterial strains isolated from diseased rainbow trout (=5) and Atlantic salmon (=3) were characterized by phenotypic and molecular taxonomic methods. The isolates were negative for the Gram-reaction, non-motile, rod-shaped and catalase- and oxidase-positive. Colonies on solid media were yellow, smooth, shiny and circular with regular edges. Growth occurred at 4–28 °C (optimum, 15 °C) and with 0–3 % NaCl (optimum, 0.5 %). Analysis of the 16S rRNA gene sequence allocated the micro-organisms to the genus , with PSD1-4 and JS6-6 as their closest relatives (96.9 and 97.1 % sequence similarity, respectively). The levels of DNA–DNA hybridization towards these nearest phylogenetic neighbours were below 17.1 %. The DNA G+C contents of strains VQ-6316s and VQ-4836s were 32.5 and 32.3 mol%, respectively. The predominant menaquinone was MK-6 and the major fatty acids were iso-C, anteiso-C, iso-C 9, iso-C 3-OH and summed feature 3 (comprising C 7 and/or C 7 and/or iso-C 2-OH). The eight isolates were classified as representatives of a novel species, sp. nov., with strain VQ-6316s (=CECT 7357=DSM 21068) as the type strain.


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vol. , part 12, pp. 3001 - 3005

Transmission electron micrograph of cells of strain VQ-6316s grown on AOA for 48 h at 15°C.

. API ZYM profiles of species of the genus .

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