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A rod-shaped, yellow-pigmented, aerobic, Gram-negative bacterium, designated strain JO-1T, was isolated from an apparently healthy Pacific oyster, Crassostrea gigas, collected at Wan Island, Korea. It grew at 15–37 °C (optimum 30 °C) only in the presence of sea salts. Strain JO-1T hydrolysed casein, Tween 80 and starch. The major fatty acids were iso-C15 : 0 (23.8 %), summed feature 3 (comprising C16 : 1 ω7c and/or iso-C15 : 0 2-OH; 14.5 %) and iso-C15 : 1 G (14.1 %). Analysis of the 16S rRNA gene sequence indicated that strain JO-1T was a member of the genus Tenacibaculum in the family Flavobacteriaceae, with sequence similarity of 94.6–97.8 % to the type strains of recognized members of the genus. The G+C content of the genomic DNA was 31.4 mol%. DNA–DNA relatedness levels between strain JO-1T and the five closest relatives, Tenacibaculum litoreum KCCM 42115T, T. lutimaris KCTC 12302T, T. aestuarii KCTC 12569T, T. mesophilum DSM 13764T and T. adriaticum JCM 14633T, were less than 28 %. Phylogenetic analyses and differences in physiological and biochemical characteristics suggested that strain JO-1T (=KCTC 22329T =JCM 15428T) should be classified as the type strain of a novel species within the genus Tenacibaculum, for which the name Tenacibaculum crassostreae sp. nov. is proposed.
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International Journal of Systematic and Evolutionary Microbiology vol. 59 , part 7, pp. 1609 - 1614
Supplementary Fig. S1. Scanning electron micrograph of cells of Tenacibaculum crassostreae sp. nov. JO-1 T. Bar, 2 µm.