1887

Abstract

A rod-shaped, yellow-pigmented, aerobic, Gram-negative bacterium, designated strain JO-1, was isolated from an apparently healthy Pacific oyster, , collected at Wan Island, Korea. It grew at 15–37 °C (optimum 30 °C) only in the presence of sea salts. Strain JO-1 hydrolysed casein, Tween 80 and starch. The major fatty acids were iso-C (23.8 %), summed feature 3 (comprising C 7 and/or iso-C 2-OH; 14.5 %) and iso-C G (14.1 %). Analysis of the 16S rRNA gene sequence indicated that strain JO-1 was a member of the genus in the family , with sequence similarity of 94.6–97.8 % to the type strains of recognized members of the genus. The G+C content of the genomic DNA was 31.4 mol%. DNA–DNA relatedness levels between strain JO-1 and the five closest relatives, KCCM 42115, KCTC 12302, KCTC 12569, DSM 13764 and JCM 14633, were less than 28 %. Phylogenetic analyses and differences in physiological and biochemical characteristics suggested that strain JO-1 (=KCTC 22329 =JCM 15428) should be classified as the type strain of a novel species within the genus , for which the name sp. nov. is proposed.

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2009-07-01
2019-10-22
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References

  1. Avendaño-Herrera, R., Toranzo, A. E. & Magariños, B. ( 2006; ). Tenacibaculosis infection in marine fish caused by Tenacibaculum maritimum: a review. Dis Aquat Organ 71, 255–266.[CrossRef]
    [Google Scholar]
  2. Bernardet, J.-F., Nakagawa, Y. & Holmes, B. ( 2002; ). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52, 1049–1070.[CrossRef]
    [Google Scholar]
  3. Choi, D. H., Kim, Y.-G., Hwang, C. Y., Yi, H., Chun, J. & Cho, B. C. ( 2006; ). Tenacibaculum litoreum sp. nov., isolated from tidal flat sediment. Int J Syst Evol Microbiol 56, 635–640.[CrossRef]
    [Google Scholar]
  4. Chun, J., Bae, K. S., Moon, E. Y., Jung, S. O., Lee, H. K. & Kim, S. J. ( 2000; ). Norcardiopsis kunsanensis sp. nov., a moderately halophilic actinomycetes isolated from a saltern. Int J Syst Evol Microbiol 50, 1909–1913.
    [Google Scholar]
  5. CLSI ( 2003; ). Performance standards for antimicrobial disk susceptibility tests, Approved Standard, 8th edn. Wayne, PA: Clinical Laboratory Standards Institute.
  6. Felsenstein, J. ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef]
    [Google Scholar]
  7. Fitch, W. M. & Margoliash, E. ( 1967; ). Construction of phylogenetic trees. Science 155, 279–284.[CrossRef]
    [Google Scholar]
  8. Frette, L., Jørgensen, N. O. G., Irming, H. & Kroer, N. ( 2004; ). Tenacibaculum skagerrakense sp. nov., a marine bacterium isolated from the pelagic zone in Skagerrak, Denmark. Int J Syst Evol Microbiol 54, 519–524.[CrossRef]
    [Google Scholar]
  9. Hansen, G. H. & Sørheim, R. ( 1991; ). Improved method for phenotypical characterization of marine bacteria. J Microbiol Methods 13, 231–241.[CrossRef]
    [Google Scholar]
  10. Hansen, G. H., Bergh, Ø., Michaelsen, J. & Knappskog, D. ( 1992; ). Flexibacter ovolyticus sp. nov., a pathogen of eggs and larvae of Atlantic halibut, Hippoglossus hippoglossus L. Int J Syst Bacteriol 42, 451–458.[CrossRef]
    [Google Scholar]
  11. Heindl, H., Wiese, J. & Imhoff, J. F. ( 2008; ). Tenacibaculum adriaticum sp. nov., from a bryozoan in the Adriatic Sea. Int J Syst Evol Microbiol 58, 542–547.[CrossRef]
    [Google Scholar]
  12. Jukes, T. H. & Cantor, C. R. ( 1969; ). Evolution of protein molecules. In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  13. Jung, S.-Y., Oh, T.-K. & Yoon, J.-H. ( 2006; ). Tenacibaculum aestuarii sp. nov., isolated from a tidal flat sediment in Korea. Int J Syst Evol Microbiol 56, 1577–1581.[CrossRef]
    [Google Scholar]
  14. Lane, D. J. ( 1991; ). 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by E. Stackebrandt & M. Goodfellow. Chichester: Wiley.
  15. Lee, J.-S., Lee, K. C., Pyun, Y.-R. & Bae, K. S. ( 2003; ). Arthrobacter koreensis sp. nov., a novel alkalitolerant bacterium from soil. Int J Syst Evol Microbiol 53, 1277–1280.[CrossRef]
    [Google Scholar]
  16. Lyman, J. & Fleming, R. H. ( 1940; ). Composition of sea water. J Mar Res 3, 134–146.
    [Google Scholar]
  17. Marmur, J. & Doty, P. ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef]
    [Google Scholar]
  18. MIDI Inc. ( 1999; ). Sherlock Microbial Identification System Operating Manual, version 3.0. Newark, DE: MIDI.
  19. Piñeiro-Vidal, M., Riaza, A. & Santos, Y. ( 2008a; ). Tenacibaculum discolor sp. nov. and Tenacibaculum gallaicum sp. nov., isolated from sole (Solea senegalensis) and turbot (Psetta maxima) culture systems. Int J Syst Evol Microbiol 58, 21–25.[CrossRef]
    [Google Scholar]
  20. Piñeiro-Vidal, M., Carballas, C. G., Gómez-Barreiro, O., Riaza, A. & Santos, Y. ( 2008b; ). Tenacibaculum soleae sp. nov., isolated from diseased sole (Solea senegalensis Kaup). Int J Syst Evol Microbiol 58, 881–885.[CrossRef]
    [Google Scholar]
  21. Piñeiro-Vidal, M., Pazos, F. & Santos, Y. ( 2008c; ). Fatty acid analysis as a chemotaxonomic tool for taxonomic and epidemiological characterization of four fish pathogenic Tenacibaculum species. Lett Appl Microbiol 46, 548–554.[CrossRef]
    [Google Scholar]
  22. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  23. Sheu, S.-Y., Lin, K.-Y., Chou, J.-H., Chang, P.-S., Arun, A. B., Young, C.-C. & Chen, W.-M. ( 2007; ). Tenacibaculum litopenaei sp. nov., isolated from a shrimp mariculture pond. Int J Syst Evol Microbiol 57, 1148–1153.[CrossRef]
    [Google Scholar]
  24. Suzuki, M., Nakagawa, Y., Harayama, S. & Yamamoto, S. ( 2001; ). Phylogenetic analysis and taxonomic study of marine Cytophaga-like bacteria: proposal for Tenacibaculum gen. nov., with Tenacibaculum maritimum comb. nov. and Tenacibaculum ovolyticum comb. nov., and description of Tenacibaculum mesophilum sp. nov. and Tenacibaculum amylolyticum sp. nov. Int J Syst Evol Microbiol 51, 1639–1652.[CrossRef]
    [Google Scholar]
  25. Swofford, D. L. ( 1998; ). Phylogenetic analysis using parsimony (paup), version 4. Sunderland, MA: Sinauer Associates.
  26. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  27. Tindall, B. J., Sikorski, J., Smibert, R. A. & Krieg, N. R. ( 2007; ). Phenotypic characterization and principles of comparative systematics. In Methods for General and Molecular Microbiology, pp. 330–393. Edited by C. A. Reddy, T. J. Beveridge, J. A. Breznak, G. A. Marzluf, T. M. Schmidt, & L. R. Snyder. Washington, DC: American Society for Microbiology.
  28. Wakabayashi, H., Hikida, M. & Masumura, K. ( 1986; ). Flexibacter maritimus sp. nov., a pathogen of marine fishes. Int J Syst Bacteriol 36, 396–398.[CrossRef]
    [Google Scholar]
  29. Wang, J. T., Chou, Y. J., Chen, C. A. & Chen, W. M. ( 2008; ). Tenacibaculum aiptasiae sp. nov., isolated from a sea anemone Aiptasia pulchella. Int J Syst Evol Microbiol 58, 761–766.[CrossRef]
    [Google Scholar]
  30. Yoon, J.-H., Kang, S.-J. & Oh, T.-K. ( 2005; ). Tenacibaculum lutimaris sp. nov., isolated from a tidal flat in the Yellow Sea, Korea. Int J Syst Evol Microbiol 55, 793–798.[CrossRef]
    [Google Scholar]
  31. ZoBell, C. E. ( 1941; ). Studies on marine bacteria. I. The cultural requirements of heterotrophic aerobes. J Mar Res 4, 42–75.
    [Google Scholar]
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vol. , part 7, pp. 1609 - 1614

Scanning electron micrograph of cells of sp. nov. JO-1 . Bar, 2 µm.



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