Ten actinomycete strains that form chains of spiral or looped spores were isolated from soil and dung samples in Japan. They contained - and -lysine, -diaminopimelic acid (Apm), -glutamic acid and - and -alanine in the cell-wall peptidoglycan, madurose as a characteristic whole-cell sugar, MK-9(H) and MK-9(H) as the major isoprenoid quinones and iso-C as the major cellular fatty acid and showed genomic DNA G+C contents of 69–74 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that the isolated actinomycete strains consistently formed a monophyletic cluster with NBRC 14099 and a separate line of descent in the phylogenetic cluster of the family . NBRC 14099 also contained - and -lysine in addition to -Apm. This genetic and phenotypic evidence revealed that the actinomycete strains could be clearly differentiated from the other members of the family and that they warranted separate genus status. We conclude that should be assigned the status of the type species of a new genus as gen. nov., comb. nov. (type strain NBRC 14099 =ATCC 27298 =BCRC 13386 =CBS 261.72 =CIP 105479 =DSM 43459 =JCM 3146 =KCTC 9252 =NCIMB 11118 =NRRL B-16128). Further, we conclude that the ten new isolates should be assigned to the novel species sp. nov. (type strain TT00-28 =NBRC 103682 =KCTC 19537), sp. nov. (type strain A3015 =NBRC 103678 =KCTC 19535), sp. nov. (type strain TT04-09 =NBRC 103688 =KCTC 19542), sp. nov. (type strain TT99-66 =NBRC 103680 =KCTC 19536), sp. nov. (type strain TT02-47 =NBRC 103685 =KCTC 19539), sp. nov. (type strain TT02-15 =NBRC 103683 =KCTC 19538), sp. nov. (type strain TTN02-30 =NBRC 103687 =KCTC 19541) and sp. nov. (type strain TTN02-22 =NBRC 103686 =KCTC 19540).


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Maximum-likelihood phylogenetic tree derived from 16S rRNA gene sequences of species and related actinomycetes belonging to the family . [PDF](56 KB)


Two-dimensional TLC of the polar lipids of strain NBRC 14099 . Chloroform/methanol/water (65:25:4, by vol.) was used in the first dimension and chloroform/methanol/acetic acid/water (80:12:15:4, by vol.) was used in the second dimension. Lipids were visualized by spraying the plates with Dittmer–Lester reagent (Dittmer & Lester, 1964). DPG, Diphosphatidylglycerol; PG, phosphatidylglycerol.


Scanning electron micrograph of growth of strain NBRC 14099 on HV agar medium (pH 6) after 3 weeks at 28 °C. Bar, 2 µm.


[PDF file of Supplementary Tables S1 and S2](83 KB)


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